SGlu2 gene expression in coats of soybean seeds

Expressão do gene SGlu2 em tegumentos contrastantes de sementes de soja

Abstracts

Glucanases can act in plant defense against biotic factors. Despite its importance, research to study the expression of genes encoding glucanases in soybean seed coats is limited. The aim of this study was to assess the relative expression of the SGlu2 gene (β-1.3-Glucanase 2), possibly involved in defense against biotic factors, in coats of seeds of four soybean genotypes. Two genotypes of black seed coats, IAC and TP, and two of yellow seed coats, BMX Potência RR and CD 202 were used. Seeds were multiplied in a greenhouse at Embrapa Clima Temperado - ETB, and the gene expression assay was performed at the Laboratório de Sementes e Biotecnologia, UFPel. Seed coat gene expression was assessed by qPCR technique in four development stages: 40, 45, 50 and 55 days after anthesis. The SGlu2 gene shows more expression in the BMX Potência RR genotype compared to other genotypes. The gene expression in the seed coat is constant in different development stages of CD 202 cultivar and IAC and TP strains, except at 45 DAA (days after application) for this latter genotype.

Glycine max; seed development stages; qPCR technique

As glucanases podem atuar na defesa das plantas contra fatores bióticos. Apesar da sua importância, pesquisas visando estudar a expressão de genes que codificam glucanases em tegumento de sementes de soja são limitadas. O objetivo do trabalho foi avaliar a expressão relativa do gene SGlu2 (β-1,3-Glucanase 2), possivelmente envolvido na defesa contra fatores bióticos, em tegumento de sementes de quatro genótipos de soja. Foram utilizados dois genótipos de tegumentos pretos, IAC e TP, e dois de tegumentos amarelos, BMX Potência RR e CD 202. As sementes foram multiplicadas em casa de vegetação na Embrapa Clima Temperado - ETB, sendo o ensaio de expressão gênica realizado no Laboratório de Sementes e Biotecnologia da UFPel. A expressão do gene no tegumento das sementes foi avaliada pela técnica qPCR, em quatro fases de desenvolvimento: 40, 45, 50 e 55 dias após a antese. O gene SGlu2 apresenta maior expressão no genótipo BMX Potência RR em relação aos demais genótipos. A expressão do gene no tegumento das sementes é constante nas diferentes fases de desenvolvimento da cultivar CD 202 e das linhagens IAC e TP, com exceção aos 45 DAA para este último genótipo.

Glycine max; fases de desenvolvimento de semente; qPCR

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Publication Dates

• Publication in this collection
  22 Aug 2014
• Date of issue
  Sept 2014

History

• Received
  28 Feb 2014
• Accepted
  18 July 2014