Correlation between serum levels of interleukins 10 and 12 and thrombocytopenia in hepatitis C cirrhotic ( class A ) patients

Hepatitis C virus (HCV) patients commonly have low platelet counts; however, the exact role of HCV infection in thrombocytopenia is unknown. This work aimed to study the serum levels of interleukins (IL) 10 and 12 in patients with mild and moderate thrombocytopenia associated with chronic hepatitis C infection. Our study included 15 patients with chronic HCV infection and newly diagnosed isolated autoimmune thrombocytopenia (Group I) and 15 patients with chronic HCV infection and normal platelet count as controls (Group II). All patients were examined for personal history and clinical aspects, complete blood count, bone marrow aspiration, liver function tests, HCV antibody assay by ELISA and polymerase chain reaction (PCR), abdominal ultrasound, Helicobacter pylori stool antigen test, evaluation of serum levels of IL-10, IL-12 and platelet specific antibodies. Our results revealed that eight patients from Group l had mild thrombocytopenia and seven patients had moderate thrombocytopenia. Serum IL-10 level was significantly elevated (t = 9.301, p < 0.001) while serum IL-12 showed a significant decrease (t = 6.502, p < 0.001) in Group I compared to the control group. No correlation was detected between platelet counts and the serum levels of either IL-10 [r = 0.454, p = 0.089 (Group I), r = 0.038, p = 0.89 (Group II)] or IL-12 [r = 0.497, p = 0.06 (Group I), r = 0.499, p = 0.058 (Group II)]. However, in Group I, a significant correlation was present only between moderate thrombocytopenia and serum levels of either IL-10 (r = 0.794, p = 0.033) or IL-12 (r = 0.967, p = 0.001), while no correlation was detected between these interleukin parameters and mild thrombocytopenia (r = 0.311 and p = 0.453 for IL-10 and r = –0.08 and p = 0.851 for IL-12). Based on our data, we may conclude that interleukins 10 and 12 are involved in low platelet levels.


INTRODUCTION
Thrombocytopenia is a common complication in patients with chronic liver disease.A platelet count less than 150.000/µL has been observed in up to 76% of patients.Moderate thrombocytopenia (platelet count ranging from 50,000 to 75,000/ µL) occurs in approximately 13% of patients with cirrhosis (1).Infection with hepatitis C virus (HCV) is a major cause of chronic liver diseases.HCV antibody-positive individuals are 2.6 times more likely to have a low platelet count than those who are HCV-antibody negative (2).
In untreated hepatitis C patients, both prevalence and severity of thrombocytopenia increase in parallel with the extent of the disease, usually becoming clinically relevant when patients develop extensive fibrosis and/or cirrhosis (3).
The pathophysiology of thrombocytopenia in patients with chronic liver disease resulting from HCV infection is complex and involves several complementary mechanisms that may act in concert.Pathogenetic mechanisms comprise hypersplenism secondary to portal hypertension, bone marrow suppression resulting from either HCV itself or interferon treatment, aberrations of the immune system provoking the formation of antiplatelet antibodies and/ or immune complexes that bind to platelets and facilitate their premature clearance, crossreactivity of antiplatelet glycoprotein antibodies and viral antigens (antigenic mimicry) (3,4).Development of immunologically-mediated extrahepatic manifestations including mixed cryoglobulinemia with or without associated joint, renal or cutaneous involvement and thrombopoietin deficiency secondary to liver dysfunction constitute another postulated mechanisms (3).
Thrombocytopenia can potentially postpone or interfere with diagnostic and therapeutic procedures including liver biopsy, antiviral therapy, and medically indicated or elective surgery (1).In the present study, we evaluated the levels of interleukins 10 and 12 in patients presenting different degrees of autoimmune thrombocytopenia associated with chronic hepatitis C infection.

PATIENTS AND METHODS
The present study included 15 patients with chronic hepatitis C virus infection associated with newly diagnosed isolated autoimmune thrombocytopenia (Group I) and 15 patients with chronic hepatitis C and normal platelet count as the control group (Group II).The two groups were matched for age and sex.All patients had liver cirrhosis Child class A. Patients with evidence of hypersplenism, previous recent therapy with interferon or ribavirin, positive serology for HBV, HIV or positive H. pylori antigen in the stool were excluded from the study.
All patients were subjected to: • Thorough history taking and clinical examination with special emphasis on immunologically-mediated extrahepatic manifestations including joint, renal or cutaneous involvement.

Statistical analysis
The SPSS package, version 9.0 for Windows® (Microsoft, USA), was used for statistical analysis of data.Student's t-test was employed to compare means of age, hematological and chemical parameters between patients and the control group.The χ 2 -test was utilized for comparison of sex distribution into Group I or Group II.Associations between the different studied variables were evaluated with Pearson's correlation (r); p ≤ 0.05 was considered significant.Data are shown as mean ± standard deviation (SD).

RESULTS
The clinical and laboratory data of the studied groups are shown, respectively, in Tables 1 and 2. Clinically evident bleeding was present in nine out of 15 (60%) patients in the form of recurrent epistaxis and gingival bleeding.
Table 3 presents the correlation between platelet counts and serum levels of interleukins 10 and 12 in HCV positive group associated with moderate thrombocytopenia.No correlation was detected between platelet count and the serum levels of either IL-10 (r = -0.038,p = 0.89 for   controls; r = 0.454, p = 0.089 for HCV positive group associated with thrombocytopenia) or IL-12 (r 0.499, p = 0.058 for controls, r = 0.497; p = 0.06 for HCV positive group associated with thrombocytopenia).However, moderate thrombocytopenia was significantly correlated with serum levels of both IL-10 (r = 0.794, p = 0.033) and IL-12 (r = 0.967, p = 0.001); although such correlation was not detected in mild thrombocytopenia (r = 0.311, p = 0.851 for IL-10 and r = -0.08,p = 0.453 for IL-12).The respective mean values of interleukins 10 and 12 in HCV positive cirrhotic patients associated with moderate thrombocytopenia were: 15.86 ± 3.89 pg/mL and 4.57 ± 4.45 pg/mL.

DISCUSSION
HCV infection may be associated with significant thrombocytopenia and appears to be a distinct clinical entity (10).Moderate thrombocytopenia was present in seven out of 15 (47%) patients while mild thrombocytopenia was detectable in eight out of 15 (53%).In a previous study of HCV-thrombocytopenic patients, 4% had severe thrombocytopenia (≤ 10 x 10 9 /L) while 74% had a maximum platelet count of 50 x 10 9 /L (10).
The mean age of our patients was 58 years in Group l and 55 years in Group II with no statistically significant difference between the two groups as to age or sex.The prevalence of thrombocytopenia in older persons with HCV infection was 6.6 times greater than that among the elderly without HCV infection and equally distributed between genders.Anti-HCV-positive older individuals were three times more likely than persons in other age groups to have thrombocytopenia.This might be related to poorly compensated platelet production, especially in individuals with severe liver disease (11).
In our study bleeding was clinically evident in nine out of 15 (60%) studied thrombocytopenic patients in the form of recurrent epistaxis and gum bleeding.The majority of older individuals with anti-HCV and thrombocytopenia are asymptomatic, but major bleeding was more frequent (10).
Similarly, in the current work, all patients had liver cirrhosis Child class A. HCV-infectionassociated thrombocytopenia was correlated with hepatocellular damage and hepatic fibrosis (11).The prevalence of thrombocytopenia increased markedly with liver disease severity among patients with HCV infection; the prevalence was about nine times higher among subjects with chronic hepatitis, cirrhosis, or hepatoma than among those with a normal or fatty liver (11).
The exact role of HCV infection in the occurrence of thrombocytopenia is unknown.An impaired thrombopoietin (TPO) production did not explain the development of thrombocytopenia in HCV infection.It was suggested that TPO production is maintained at certain levels until profound impairment of hepatic function has developed, when other organs may compensate the decrease in TPO production by the liver (2).Therefore, other mechanisms must be involved.
Dysregulation of T-helper (Th1/Th2) cytokine production may play an important role in immunopathogenesis of chronic hepatitis C (CHC) (12).In CHC patients, interferon-γ (IFN-γ) and IL-12 levels drop as a result of the enhanced IL-10 production, which serves as a possible down-regulator of IFN-γ (13).Further research on the role of these cytokines in HCV infection associated with thrombocytopenia may clarify additional functions of these cytokines.
IL-10 is an important immunoregulatory cytokine and its main biological functions seem to be the limitation and termination of inflammatory responses and the regulation of differentiation and proliferation of T cells, B cells, natural killer cells, antigen-presenting cells, mast cells and granulocytes.Moreover, IL-10 promotes the development of a type 2 cytokine pattern by inhibiting IFN-γ production (14).
The present study revealed statistically significant elevation in serum IL-10 levels in Group I (HCV associated with thrombocytopenia) when compared with Group II.HCV-specific IL-10 response was detected within one to two months of acute clinical hepatitis C (15).IL-10 has been reported to be one of the potential factors in establishing immune suppression and viral persistence (16).The present study revealed statistically significant decrease in IL-12 levels in Group I in relation to controls.
IL-12 is a proinflammatory cytokine produced by dendritic cells and phagocytes in response to pathogens during infection (17).It plays a central role in coordinating innate and adaptive immunity.Expression of IL-12 during infection regulates innate responses and determines the type and duration of adaptive immune response.Additionally, this cytokine induces IFN-γ production by natural killer cells, T cells, dendritic cells and macrophages, and promotes the differentiation of naive CD4+ T cells into Th1 cells that produce IFN-γ and aid in cell-mediated immunity (18).
In the present study, no correlation was detected between platelet counts and serum levels of either IL-10 or IL-12 in Group I or II.However, in Group I, a significant correlation was detected between moderate thrombocytopenia and serum levels of both IL-10 and IL-12, although this connection was not present in mild thrombocytopenia.Elevated IL-10 serum levels in chronic HCV patients were not associated with different laboratory parameters, histological activity index or HCV-RNA viral load for any of the patient population (19).

CONCLUSIONS
The present results reveal the presence of a function of interleukins 10 and 12 at lower platelet levels together with other factors.Further studies are still required to confirm and explain this relationship.If these findings are confirmed, these cytokines may be a marker of moderateto-severe thrombocytopenia in chronic HCV patients.

Table 1 .
Clinical data of the studied groups

Table 2 .
Laboratory data of the studied groups Group l: patients with chronic-hepatitis-C-infection-associated thrombocytopenia; Group ll: patients with chronic hepatitis C infection and normal platelet count (control group); p is significant if ≤ 0.05.

Table 3 .
Correlations between platelet counts and interleukins 10 and 12 in HCV-positive cirrhotic patients associated with moderate thrombocytopenia p is significant if ≤ 0.05.