PULMONARY TUBERCULOSIS: HEMATOLOGY, SERUM BIOCHEMISTRY AND THE RELATION WITH THE DISEASE DURATION OLIVA

The aim of the present study was to analyze the relationship between hematological and biochemical parameters and tuberculosis process activity time according to clinical complaint duration. It was a retrospective study analyzing medical records from 80 pulmonary tuberculosis patients at Botucatu Medical School University Hospital (Botucatu, São Paulo State, Brazil), who were divided into 2 groups according to clinical complaint duration: Group 1 (G1) – up to three months; Group 2 (G2) – over three months. Parameters included: age, gender, bacilloscopy, erythrocyte sedimentation rate (ESR), platelet count, alpha1-globulin, alpha2globulin, gamma globulin, mucoprotein, alpha1-acid glycoprotein values, and the presence of risk factors such as smoking, alcoholism, drug addiction, sexual promiscuity, contact with tuberculosis carriers, and previous treatment. Groups were compared by calculating t and p, and Chi-square (X) and p. Comparisons revealed a tendency towards smoking with a higher frequency of smokers in G1 (0.05<p<0.10). G1 also tended to present greater platelet values than G2 (0.05<p<0.10) and presented significantly higher ESR values than G2 (p<0.05). Other factors did not show any significantly different behavior between groups (p>0.05). A correlation was found between ESR, platelet count, smoking and less than three months clinical duration.


INTRODUCTION
Tuberculosis (TB) is a highly prevalent chronic infectious disease caused by Mycobacterium tuberculosis, an aerobic intracellular binding bacterium (bacillus); because of this characteristic it prefers tissues which are always in contact with high oxygen levels, as in the lung.After inhaling the bacillus, transmitted by tiny droplets of saliva, the infected individual may develop the disease depending on his immunological state (1,16).After taking up residence in the lung, M. tuberculosis can disseminate to any part of the organism (16).
Globally, M. tuberculosis infection remains at epidemic levels (18).One third of the world's population is infected and approximately 3 million people die annually from pulmonary tuberculosis, overtaking the number of deaths due to acquired immune deficiency syndrome (AIDS), malaria, diarrhea, leprosy, and other tropical diseases combined (26).In Brazil, there are about 100,000 new cases and 6,000 deaths reported every year (9).This makes tuberculosis the 4 th highest cause of death from infectious diseases, and even though treatment is performed on an outpatient basis, it is the 9 th highest cause of hospitalization from infectious diseases and the 7 th highest in cost from these hospitalizations (7).
Around 10% of tuberculosis cases are in the under 20s, with the most affected age group being the 20-49-year-old, accounting for 70% of all those affected.It affects three times as many men as women (21).

Routine diagnosis of pulmonary and extra-pulmonary tuberculosis is by bacilloscopy
(BAAR); it is an easy, quick and safe method to justify starting treatment.However, a more specific and sensitive method is mycobacteria isolation in culture medium, which has the disadvantage of slow bacteria growth resulting in longer diagnosis time (13).
Initially, combat against M. tuberculosis is mediated by inflammatory cytokines such as IL-1, IL-2, and mainly TNF-α; these are essential for controlling acute infection by both local inflammatory process and macrophage (microbicide mechanisms) activation (14,30).TNF-α, produced by alveolar macrophages, increases nitric oxide (NO) expression and inducible nitric oxide synthase enzyme (iNOS), favoring granuloma maintenance and integrity (19).Excess TNF-α associated with IL-1 could be related to disease pathogenesis, as these cytokines cause fever, tissue necrosis, and cachexia (17).
IL-2 and IFN-γ secretion which characterizes Th1 profile is seen during initial infection stages and Th2 profile, characterized by IL-4, IL-5, IL-6, and IL-10 secretion, is apparent in later stages of the disease (12).
Bacillus survival inside macrophages causes the immune response of immunocompetent individuals to constantly activate T lymphocytes which will produce large quantities of INF-γ and TNF-α leading to the accumulation of macrophages and lymphocytes, which possibly form the granuloma and latent foci.In immunosupression states, these foci may be reactivated and the infection progress (8,11).
Cytokines are responsible for clinical and laboratory alterations which occur during the inflammatory process, such as fever, leukocytosis, thrombocytosis, and acutephase hepatic responses (2).In tuberculosis, for example, the inflammatory process has increased concentrations of several serum proteins, called acute-phase proteins.These include α1glycoprotein acid, mucoprotein, α1-globulin, α2-globulin, and the γ-globulins (4).
Mucoprotein is a glycoprotein rich in acid polysaccharides, the main component being α1-glycoprotein acid, the inflammatory activity marker responsible for performing immunomodulatory activities (23,28).The globulins (α, β and γ) influence ESR, a non-specific test for inflammatory and infectious processes used to evaluate activity level and disease extent, response to treatment, and the prognosis of subacute and chronic diseases such as tuberculosis (22,25,29).
A relevant hematological parameter in pulmonary tuberculosis is platelet count.
When high, it characterizes an abnormal fibrinolytic system which leads to hypercoagulability (6,15).The objective of this work was to analyze the relationship of hematological and biochemical parameters with tuberculosis process activity according to clinical duration.Data comparisons between the two groups were made using t and p statistics; and Chi squared (x 2 ) and p calculations.Values were considered significant when p<0.05; and there was a tendency towards significance when 0.05<p<0.10.

This study was approved by the Human Research Ethics Committee of Botucatu
Medical School, UNESP.
Of the 80 patients in this series, 49 (61.25%)presented positive sputum bacilloscopy without significant difference between groups (G1: 53.06% positivity & G2: 46.94%; Table 2) Of the pulmonary tuberculosis risk factors, Figure 1 shows smoking as the most frequently reported with a total of 59 (73.75%) confirming they were smokers; 34 from G1 (57.63%) and 25 (42.37%) from G2.There was a tendency between groups towards more smokers with less than three months clinical duration (G1).
Only 11 patients reported the use of illicit drugs and sexual promiscuity; however, there was no significant difference between groups (Figure 1).

DISCUSSION
Tuberculosis is a highly important global chronic disease and is responsible for more deaths than other tropical diseases.Brazil has high annual incidence and mortality rates (1,9,16,26).
The higher frequency of male individuals in both groups was in line with other studies (13,21,27).Causes of this predominance in men in relation to women have been cited as biological differences such as immunity, exposure to M. tuberculosis associated with different social miscegenation profiles and social behaviors including smoking (27).
G1 and G2 mean ages were higher than the Brazilian average; this is probably due to them being referred to a high complexity treatment unit (13,21).(5,13,27).This low sensitivity could be due to the slow growth of the bacillus, or the result of infection controlled by the individual (13).
For smoking, a tendency to higher frequency was seen in the up-to-three-months group (G1≥G2).The predominance of smokers in G1 reflects their lower tolerance as smoking is implicated in structural and immunological alterations of organism defense such as rupture of the respiratory epithelium, changes in pathogen adherence, prejudiced mucociliary depuration, deficiency in macrophage phagocyte activity, and CD 4 + T lymphopenia, increasing the susceptibility to pulmonary tuberculosis (3).
Alcoholism must also be highlighted even though this study did not observe any significant difference between groups.It is considered a factor that reduces organic resistance as the alcoholic does not feed himself adequately, is inclined towards gastritis which can lead to lack of appetite which can end up compromising individual nutritional state and resistance (10,20).Frequent infections of the respiratory tract and inspired pneumonia also significantly contribute to the high incidence of chronic lung disease in alcoholics (20).The importance of alcoholism in tuberculosis determinism for this population can be inferred by the fact that half of them have a previous history of alcohol abuse.
For this population, drug addiction was not a risk factor, probably because of their ages, as mean ages (45 years for G1 and 47 years for G2) were in general much higher than those observed for drug users.
Sexual promiscuity and contact with tuberculosis, important risk factors in the epidemiology of the disease, were not very relevant in this study and were not significant between groups.
In relation to ESR and platelet count, values were higher in those with less clinical disease duration (G1), as at the beginning of the tuberculosis process there is strong pro-inflammatory cytokine activity (IFN-γ & TNF-α) which stimulates expression of acute-phase proteins and thrombocytosis.With disease evolution, anti-inflammatory cytokine (IL-10, TGF-β) expression increases, which promote cooling down of inflammatory activity (16,30).Also, α2 and γ-globulin values are much higher than normal in patients with less disease duration, although not statistically significant (2,3,24).
Finally, this study showed some degree of relationship between smoking habit, higher ESR values and platelet counts in tuberculosis patients who presented less clinical duration.Prospective studies are necessary to confirm this relationship.
study analyzed 80 patients diagnosed with pulmonary tuberculosis and treated at the Infectious and Parasitic Diseases Service of the University Hospital, Botucatu Medical School, São Paulo State University -UNESP, Brazil, between January 1992 and December 2003.
V. M. Oliva et al.PULMONARY TUBERCULOSIS: HEMATOLOGY, SERUM BIOCHEMISTRY AND THE RELATION WITH THE DISEASE DURATION.J. Venom.Anim.Toxins incl.Trop.Dis., 2008, 14, 1, p. 74 Patients were divided into two groups according to clinical disease duration: G1, up to three-month duration (n 1 =41); and G2, over three-month duration (n 2 =39).The three-month-group split point is based on the mean time that patients seek medical attention in the above Service.Patient data were obtained by analyzing medical records considering age, gender, bacilloscopy results, ESR, platelet count, and acute-phase serum protein counts; the latter being α-1 globulin, α-2 globulin, γ-globulin, mucoprotein and α-1 glycoprotein acid.The following tuberculosis risk factors were also noted: smoking, alcoholism, drug abuse, sexual promiscuity, contact with tuberculosis, and previous antituberculosis treatment.

Table 1 .
Gender distribution of patients with pulmonary tuberculosis.

Table 3 .
Analysis of hematological and biochemical parameters of patients with pulmonary tuberculosis.
RELATION WITH THE DISEASE DURATION.J. Venom.Anim.Toxins incl.Trop.Dis., 2008, 14, 1, p. 78 Bacilloscopy behavior was not significantly different between groups.This method gave a 61.25% positivity which demonstrates its low sensitivity; this is in line with current bacteriological diagnosis of tuberculosis in Brazil