Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni

Livia Pica-Mattoccia Luiz Candido de Souza Dias Donato Cioli About the authors

Abstract

The objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.

Schistosoma mansoni; hycanthone resistance; genetic complementation


ABSTRACT

Genetic complementation analysis of two independently isolated hycanthone-resistant strains of Schistosoma mansoni

Livia Pica-Mattoccia1

Luiz Candido de Souza Dias2

Donato Cioli1

Institute of Cell Biology, Rome, Italy

Universidade Estadual de Campinas, Campinas, Brasil

The objective of this study is to determine whether various hycanthone resistant strains of schistosomes which have been independently isolated are all affected in the same gene. A strain obtained from a Brazilian patient was compared with a strain of Puerto Rican origin selected in the laboratory. If the mutation conferring resistance involved two different genes, one would expect that the progeny of a cross between the two strains would show complementation, i.e. it would be sensitive to the drug. We have performed such a cross and obtained F1 hybrid worms wich were essentially all resistant, thus suggesting that the mutation conferring resistance in the two strains involves the same gene.

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Publication Dates

  • Publication in this collection
    04 June 2009
  • Date of issue
    1992
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