Histochemical methods for the identification of leukocytes and macrophages

Abstract

The authors report techniques to detect acid-phosphatase, aplha-naphthyl esterase and peroxidase activity in lymphocytes and macrophages in cutaneous lesions of Leprosy. They recomend fixation in formol sucrose buffered followed by inclusion in gumma of Holt as the best method. This method mantain the activity and show sharply the details of cells.