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alpha-glycerophosphate dehydrogenase activity in flight muscles of triatomine bugs Panstrongylus megistus and Triatoma sordida

Abstract

The alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier insects than P. megistus. A possible role of alpha-GPDH on triatomines flight is discussed.

triatomines; flight activity; alpha-glycerophosphate dehydrogenase


SHORT COMMUNICATION

a-Glycerophosphate Dehydrogenase Activity in Flight Muscles of Triatomine Bugs Panstrongylus megistus and Triatoma sordida

Vol. 95(5): 707-709, Sep./Oct. 2000

Rodrigo Pedro Pinto Soares/+ + Corresponding author. Fax: + 55-31-295.3115. E-mail: rodrigo@cpqrr.fiocruz.br , Marcelo Matos Santoro*

Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil *Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brasil

The a-glycerophosphate dehydrogenase (a-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier insects than P. megistus. A possible role of a-GPDH on triatomines flight is discussed.

Key words: triatomines - flight activity - a-glycerophosphate dehydrogenase

Triatomine bugs (Hemiptera: Reduviidae: Triatominae) are the vectors of Trypanosoma cruzi, the causative agent of Chagas disease in Latin America. In recent years many efforts have been made to control the vectors of T. cruzi in Brazil and in other countries. Although these attempts have been successful there is concern that new triatomine vector species might take the place of the ones being exterminated. The ability of triatomines to disperse between different habitats may have important consequences on the reinfestation of dwellings and control programmes directed against them.

There are many records of sylvatic species of Triatominae flying into houses at night, apparently attracted by the light; also, several species seem to be progressively adapting from sylvatic to domestic habitats (Noireau et al. 1995, Valente et al. 1995). Panstrongylus megistus occupies a range of sylvatic habitats in Brazil but frequently colonizes houses and peridomestic ecotopes (Forattini et al. 1977), while Triatoma sordida has an even wider range of sylvatic and peridomestic habitats and is thought to be in the process of domiciliation (Diotaiuti et al. 1993). Both laboratory and field experiments, show that flight initiation is influenced primarily by low nutritional status and above average temperatures (Lehane et al. 1992). Nevertheless, there are invariably some bugs that do not initiate flight even under apparently optimum conditions.

Cytoplasmic a-glycerophosphate dehydrogenase (a-GPDH) is involved in a variety of functions in both carbohydrate and lipid metabolism. In the Diptera order, a-GPDH is particularly important in the glycerophosphate cycle of flight muscle, through which glycolysis is coupled to oxidative phosphorylation. This cycle regenerates NAD+ in the cytoplasm and provides reducing equivalents as FADH2 for ATP synthesis within the mitochondria (Sacktor 1970). In wasps and bees, a-GPDH synthesis correlates with age and flight capacity (Machado & Contel 1991), while in Drosophila thoracic a-GPDH activity increases gradually as the adult insect becomes able to fly (Sullivan et al. 1983). Demonstration of the a-GPDH role in Drosophila flight was emphasised by O'Brien and MacIntyre (1972) who showed that flies bearing a mutation at the a-GPDH locus were unable to fly. In the Triatominae, a-GPDH is one of the few glycolytic enzymes showing a degree of polymorphism (Dujardin et al. 1998) and our aim in this work was to see if there was any correlation between a-GPDH activity and the flight capacity of Triatominae. For this study we used adult P. megistus and T. sordida from different regions of Brazil. The P. megistus colony originated from the State of Minas Gerais, but had been reared for several years under laboratory conditions (27 ± 2°C, 60 ± 5% RH), whereas T. sordida was captured from peridomestic habitats in Serra do Ramalho, State of Bahia, Brazil. Fifty males and 50 females from each population were used in each experiment. The bugs were fed for 30 min on anaesthetized mice and the following day were placed in an actograph (Lehane & Schofield 1981) under controlled laboratory conditions (28 ± 1°C and 42 ± 2% RH). The insects were observed daily for a period of 30 days, in order to separate fliers from non-fliers. Those that flew were removed and stored in liquid nitrogen prior to extraction of thoracic muscles. Insects that did not fly after 30 days were considered non-fliers and were also stored in liquid nitrogen. Frozen bugs had their thoracic flight muscles removed, homogenized with 100 µl of enzyme stabilizer (2mM dithiotreitol, 2mM aminocaproic acid and 2mM EDTA, pH 7.3) and centrifuged at 15,000 g at 4ºC. Activity of a-GPDH was assayed spectrophotometrically at 25ºC, following the production of NADH at 340 nm upon substrate addition (Chambers et al. 1985). Protein concentrations were determined as described by Bradford (1976), using bovine serum albumin as the standard. The specific activity is given in Units/mg of protein in muscle extracts.

In this experiment, the flight capacity of T. sordida (52% fliers) was higher than that of P. megistus (15% fliers), and a-GPDH activity was significantly higher in fliers than in non-fliers in both species (Students t-test, p<0.05) (Table).

Table

The results of previous studies of sustained flight in triatomines suggest that the main source of flight energy is derived from oxidation of lipid and that the utilization of carbohydrates may take place only during the first minutes of flight, since glycogen content in flight muscles is rather low (Ward et al. 1982). a-GPDH has a central role in lipid and carbohydrate metabolism, and in coupling the two processes (Bewley & Miller 1979). One of its most important functions is to reoxidize NADH when flight muscle activity is based on glycolysis, but when tri-acyl glycerides are the flight fuel, this enzyme also acts to convert glycerol-3-phosphate derived from glycerol into dihidroxyacetone phosphate, a glycolytic intermediate. Our experimental design considered only flight initiation and not sustained flight, but the higher a-GPDH activity in flying insects suggests that this enzyme may play an important role in triatomine flight and may be a useful marker to assess the proportion of potential fliers in natural populations of Triatominae.

REFERENCES

Table

This work was supported by CNPq and Fiocruz, Brazil, and benefited from international collaboration through the ECLAT network.

Received 18 October 1999

Accepted 23 February 2000

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  • +
    Corresponding author. Fax: + 55-31-295.3115. E-mail:
  • Publication Dates

    • Publication in this collection
      10 Oct 2001
    • Date of issue
      Oct 2000

    History

    • Accepted
      23 Feb 2000
    • Received
      18 Oct 1999
    Instituto Oswaldo Cruz, Ministério da Saúde Av. Brasil, 4365 - Pavilhão Mourisco, Manguinhos, 21040-900 Rio de Janeiro RJ Brazil, Tel.: (55 21) 2562-1222, Fax: (55 21) 2562 1220 - Rio de Janeiro - RJ - Brazil
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