Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens

Armando Menezes-Neto Marcia da Costa Castilho Guilherme Amaral Calvet Edna Oliveira Kara Camila Helena Aguiar Bôtto-Menezes Marcus Vinícius Guimarães Lacerda Gerson Fernando Mendes Pereira Silvana Pereira Giozza Ximena Pamela Diaz Bermudez Noemia Santana Lima Kayvon Modjarrad Nathalie Broutet Ana Maria Bispo de Filippis Rafael Freitas Oliveira Franca Study Team ZIKABRAAbout the authors

We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.

Key words:
Zika virus; diagnosis; persistence

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