Use of an indirect haemagglutination test, for the detection of Clostridium perfringens type A enterotoxin

Abstract

An indirect haemagglutination (IH) test is described for the detection of Clostridium perfringens type A enterotoxin, produced by strains isolated from human cases of food poisoning and from contaminated food. Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the ILM one. No unspecific reaction was obtanined int he IH wirh a negative control and the inhibitions of the IH and IIM tests by specific antiserum against C. perfringens enterotoxin showed that the IH test is very spcific. The IH assay is recommended for its sensitivity and easy performance by less-equipped laboratories, by these and other data.

Clostridum perfrigens; enterotoxin; indirect; haemagglutination


ABSTRACT

Use of an indirect haemagglutination test, for the detection of Clostridium perfringens type A enterotoxin

Marlene B. Serafim1

Isildinha A. G. Colli1

Lucila Costallat Ricci1

Antonio F. Pestana de Castro1

Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Microbiologia, Campinas, Brasil

An indirect haemagglutination (IH) test is described for the detection of Clostridium perfringens type A enterotoxin, produced by strains isolated from human cases of food poisoning and from contaminated food. Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the ILM one. No unspecific reaction was obtanined int he IH wirh a negative control and the inhibitions of the IH and IIM tests by specific antiserum against C. perfringens enterotoxin showed that the IH test is very spcific. The IH assay is recommended for its sensitivity and easy performance by less-equipped laboratories, by these and other data.

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Publication Dates

  • Publication in this collection
    15 June 2009
  • Date of issue
    Mar 1991
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