Role of a mouse monoclonal IgE antibody in passive transfer of immunity to Schistosoma japonicum infection

Somei Kojima Tuenta Janecharut Hidekazu Hata Munetoshi Niimura About the authors

Abstract

We have been able to produce a mouse monoclonal IgE antibody specific to an adult worm antigen extracted from Schistosoma japonicum (Sj). The antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against Sj with the highest titer of 1:256,000 but did not cross-react with S. mansoni antigen. The antibody recognized a 97-kDa molecule expressed on the surface of mechanically transformed schistosoma of S. japonicum. Passive transfer of the antibody into mice in the early stage of challenge infection resulted in a partial but significant reduction of recovery of adult worms. Induction of eosinophilia by an oral administration of embryonated eggs of Toxocara canis prior to challenge infection enhanced the reduction.


ABSTRACT

Role of a mouse monoclonal IgE antibody in passive transfer of immunity to Schistosoma japonicum infection

Somei Kojima1

Tuenta Janecharut1

Hidekazu Hata1

Munetoshi Niimura1

Chiba University, School of Medicine, Department of Parasitology, Chiba, Japan

We have been able to produce a mouse monoclonal IgE antibody specific to an adult worm antigen extracted from Schistosoma japonicum (Sj). The antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against Sj with the highest titer of 1:256,000 but did not cross-react with S. mansoni antigen. The antibody recognized a 97-kDa molecule expressed on the surface of mechanically transformed schistosoma of S. japonicum. Passive transfer of the antibody into mice in the early stage of challenge infection resulted in a partial but significant reduction of recovery of adult worms. Induction of eosinophilia by an oral administration of embryonated eggs of Toxocara canis prior to challenge infection enhanced the reduction.

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Publication Dates

  • Publication in this collection
    26 June 2009
  • Date of issue
    1987
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