Description of Leishmania (Leishmania) forattinii sp. n., a new parasite infecting opossums and rodents in Brazil

Elizaide L. A. Yoshida Cesar A Cuba Cuba Raquel da Silva Pacheco Elisa Cupolillo Celso Cruz Tavares Gerzia M. C. Machado Hooman Momen Gabriel Grimaldi Junior About the authors

Abstract

A new parasite species of Leishmania is described, L. (Leishmania) forattinii sp. n., which was isolated from a pooled triturate of liver and spleen of a opossum (Didelphis marsupialis aurita) and from skin samples from a rodent (Proechmys iheringi denigratus), captured in primary forest on the Atlantic Cost of Brazil. Our results on the basis of biological and molecular criteria indicate that this taxonomically distinct parasite ias a new species of the L. mexicana complex, but closely related to L. (L.) aristidesi Laison & shaw, 1979, as revelated by phenetic and phylogenetic numerical analyses of the enzyme data. L. forattinii was clearly distinguishable from other Leishmania species of the genus usisng enzyme electrophoresis, monoclonal antibodies, molecular karyotypes, analysis of restriction enzyme digestion patterns of kinetoplast DNA (kDNA), as well as the use of kDNA hybridization procedures.

Leishmania (L.) forattinii sp. n.; Protozoa; Kinetoplastida; Trypanosomatidae; mammalian resevoirs; molecular characterization; monoclonal antibodies; isoenzyme electrophoresis; kenetoplast DNA analysis; molecular karyotype analysis


ABSTRACT

Description of Leishmania (Leishmania) forattinii sp. n., a new parasite infecting opossums and rodents in Brazil

Elizaide L. A. Yoshida1

Cesar A Cuba Cuba2

Raquel da Silva Pacheco3

Elisa Cupolillo3

Celso Cruz Tavares4

Gerzia M. C. Machado5

Hooman Momen3

Gabriel Grimaldi Junior5

Universidade Estadual Paulista, IBBMA, Departamento de Parasitologia, Botucatu, Brasil

Unviersidade de Brasília, FCS, Departamento de Patologia, Brasília, Brasil

Instituto Oswaldo Cruz, Departamento de Bioquímica e Biologia Molecular, Rio de Janeiro, Brasil

Universidade Estadual do Rio de Janeiro, IB, Departamento de Biologia Celular e Genética, Rio de Janeiro, Brasil

Instituto Oswaldo Cruz, Departamento de Imunologia, Rio de Janeiro, Brasil

A new parasite species of Leishmania is described, L. (Leishmania) forattinii sp. n., which was isolated from a pooled triturate of liver and spleen of a opossum (Didelphis marsupialis aurita) and from skin samples from a rodent (Proechmys iheringi denigratus), captured in primary forest on the Atlantic Cost of Brazil. Our results on the basis of biological and molecular criteria indicate that this taxonomically distinct parasite ias a new species of the L. mexicana complex, but closely related to L. (L.) aristidesi Laison & shaw, 1979, as revelated by phenetic and phylogenetic numerical analyses of the enzyme data. L. forattinii was clearly distinguishable from other Leishmania species of the genus usisng enzyme electrophoresis, monoclonal antibodies, molecular karyotypes, analysis of restriction enzyme digestion patterns of kinetoplast DNA (kDNA), as well as the use of kDNA hybridization procedures.

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Publication Dates

  • Publication in this collection
    01 June 2009
  • Date of issue
    Sept 1993
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