A newly bat-borne hantavirus detected in Seba’s short-tailed bats (<i>Carollia perspicillata</i>) in the Brazilian Atlantic Rainforest

Souza, Patrick Jesus de; Fernandes, Jorlan; Coelho, Thayssa Alves; Cosentino, Matheus; D’arc, Mirela; Alves, Patrícia Dias Galvão; Guterres, Alexandro; Vilar, Emmanuel Messias; Lemos, Elba Regina Sampaio de; Cordeiro-Estrela, Pedro; Santos, André Felipe Andrade; Oliveira, Renata Carvalho de

doi:10.1590/0074-02760240132

Abstract

BACKGROUND Bat-borne hantaviruses have been identified worldwide but little is known about neotropical bats in the megadiverse biomes of the American continent. Although serological evidence has hinted at hantavirus circulation in Brazil, the scarce number of genomic detection represents a gap to understand viral diversity, prevalence, and ecology of bat-borne hantaviruses.

OBJECTIVE We aim to investigate and evaluate the presence and prevalence of bat-borne hantavirus in the Brazilian Atlantic Forest.

METHODS Here in, 97 lung and kidney tissue samples from bats captured in the Brazilian Atlantic Rainforest were submitted to hantavirus-specific nested reverse transcription-polymerase chain reaction (RT-PCR) targeted the hantaviral L segment and metagenomic analysis.

FINDINGS Hantavirus RNA was detected in five tissue fragments of 20 Seba’s short-tailed bats (Carollia perspicillata). Phylogenetic analysis, based on partial L-segment sequence using maximum likelihood method, demonstrated that the identified virus formed a monophyletic clade and a highly divergent bat-borne lineage comprising other recent strains found in the genus Carollia from South America.

MAIN CONCLUSIONS Our findings suggest the presence of a novel bat-borne hantavirus in Brazil, tentatively named Mamanguape virus (MGPV). Additional genomic data will help to extend our knowledge about the classification of MGPV within the Hantaviridae family and the evolution origins of new world bat-borne hantaviruses.

Key words:
bat-borne hantavirus; Brazilian Atlantic Rainforest; hantavirus; Carollia perspicillata ; chiroptera

[TFN1] RT-PCR: reverse transcription-polymerase chain reaction.

Family	Species	Abundance	Individuals tested by RT-PCR
Phyllostomidae	Artibeus cinereus	39	19
	Artibeus lituratus	19	17
	Artibeus planirostris	26	22
	Carollia perspicillata	90	20
	Desmodus rotundus	02	02
	Glossophaga soricina	12	11
	Platyrrhinus lineatus	08	03
Vespertilionidae	Myotis sp.	03	03
	Total	199	97

Virus/%*	MAMANGUAPE PP258969.1	MAMANGUAPE PP258967.1	MAMANGUAPE PP258970.1	BURITIENSE	MOUYASSUÉ	KIWIRA	MAMANGUAPE PP258966.1	MAMANGUAPE PP258968.1	HNTV1_BOL	HNTV1_BRA	HNTV1_BRA	HNTV2_MEX	HNTV2_MEX	HNTV3_MEX	BRNO	LONGQUAN	QUEZON	ROBINA
MAMANGUAPE PP258969.1	-	99,3%	96,0%	87,3%	70,2%	76,0%	98,9%	98,9%	89,4%	87,8%	87,8%	84,0%	84,4%	72,2%	73,5%	73,5%	70,2%	64,7%
MAMANGUAPE PP258967.1	98,9%	-	94,9%	87,1%	69,4%	74,4%	99,2%	98,9%	88,0%	87,5%	87,5%	82,6%	82,3%	71,2%	74,4%	73,3%	71,9%	64,5%
MAMANGUAPE PP258970.1	95,6%	92,7%	-	84,2%	66,9%	72,8%	95,6%	95,3%	86,0%	84,3%	84,3%	79,5%	79,2%	68,9%	71,1%	71,1%	68,6%	63,6%
BURITIENSE	98,9%	99,2%	92,5%	-	70,5%	73,8%	87,5%	87,1%	85,8%	92,9%	93,4%	81,5%	80,6%	71,8%	73,0%	74,4%	68,9%	66,4%
MOUYASSUÉ	82,6%	80,2%	75,8%	80,2%	-	71,3%	70,3%	70,0%	68,4%	68,7%	69,2%	67,8%	68,1%	65,8%	71,6%	70,8%	67,5%	66,7%
KIWIRA	80,4%	81,0%	76,7%	81,8%	76,0%	-	75,0%	74,7%	72,9%	72,1%	71,8%	70,9%	70,9%	68,7%	71,9%	70,5%	71,1%	69,7%
MAMANGUAPE PP258966.1	98,9%	99,2%	92,5%	100,0%	80,0%	81,7%	-	99,7%	88,3%	87,7%	87,7%	82,6%	82,3%	71,8%	74,4%	72,8%	72,0%	64,4%
MAMANGUAPE PP258968.1	98,9%	98,3%	91,7%	99,2%	80,2%	81,0%	99,2%	-	88,0%	87,5%	87,5%	82,3%	82,1%	71,8%	74,1%	72,5%	71,7%	64,2%
HNTV1_BOL	98,9%	100,0%	93,2%	99,1%	79,5%	80,3%	99,1%	98,3%	-	87,5%	86,9%	82,6%	82,3%	73,5%	71,2%	74,4%	67,2%	67,5%
HNTV1_BRA	97,7%	98,3%	91,5%	99,1%	80,3%	82,1%	99,1%	98,3%	98,3%	-	98,9%	80,6%	79,8%	73,2%	73,8%	72,4%	69,0%	65,5%
HNTV1_BRA	97,7%	98,3%	91,5%	99,1%	80,3%	82,1%	99,1%	98,3%	98,3%	100,0%	-	79,8%	79,5%	72,9%	73,8%	72,9%	68,7%	65,5%
HNTV2_MEX	96,6%	96,6%	90,6%	97,4%	81,2%	80,3%	97,4%	96,6%	96,6%	96,6%	96,6%	-	98,9%	72,4%	71,2%	70,9%	65,5%	65,2%
HNTV2_MEX	96,6%	94,9%	89,7%	95,7%	80,3%	79,5%	95,7%	94,9%	94,9%	94,9%	94,9%	98,3%	-	72,4%	70,9%	70,7%	65,2%	64,7%
HNTV3_MEX	81,8%	81,2%	76,9%	81,2%	76,1%	75,2%	81,2%	81,2%	81,2%	82,1%	82,1%	81,2%	80,3%	-	67,8%	68,9%	71,0%	66,4%
BRNO	78,3%	77,7%	73,3%	78,5%	79,3%	76,0%	78,3%	78,5%	76,9%	78,6%	78,6%	77,8%	76,9%	76,1%	-	77,1%	70,8%	67,8%
LONGQUAN	78,3%	77,7%	73,3%	76,9%	79,3%	71,9%	76,7%	76,9%	76,9%	76,9%	76,9%	75,2%	74,4%	76,9%	81,0%	-	66,9%	70,2%
QUEZON	75,8%	77,5%	72,3%	78,3%	78,3%	78,3%	78,2%	77,5%	76,7%	78,4%	78,4%	77,6%	77,6%	76,7%	76,7%	75,0%	-	67,8%
ROBINA	71,7%	73,6%	70,8%	72,7%	75,2%	76,9%	72,5%	71,9%	72,6%	72,6%	72,6%	71,8%	70,9%	74,4%	73,6%	71,9%	80,0%	-

A newly bat-borne hantavirus detected in Seba’s short-tailed bats (Carollia perspicillata) in the Brazilian Atlantic Rainforest

Abstract