Antimicrobial activity and chemical investigation of Brazilian Drosera

Abstract

The antimicrobial activity of three different extracts (hexanic, ethyl acetate, methanol) obtained from Brazilian Drosera species (D. communis, D. montana var. montana, D. brevifolia, D. villosa var. graomogolensis, D. villosa var. villosa, Drosera sp. 1, and Drosera sp. 2 ) were tested against Staphylococcus aureus (ATCC 25923), Enterococcus faecium (ATCC23212), Pseudomonas aeruginosa (ATCC27853), Escherichia coli (ATCC11229), Salmonella choleraesuis (ATCC10708), Klebsiella pneumoniae (ATCC13883), and Candida albicans (a human isolate). Better antimicrobial activity was observed with D. communis and D. montana var. montana ethyl acetate extracts. Phytochemical analyses from D. communis, D. montana var. montana and D. brevifolia yielded 5-hydroxy-2-methyl-1,4-naphthoquinone (plumbagin); long chain aliphatic hydrocarbons were isolated from D. communis and from D. villosa var. villosa, a mixture of long chain aliphatic alcohols and carboxylic acids, was isolated from D. communis and 3b-O-acetylaleuritolic acid from D. villosa var. villosa.

Drosera species; Droseraceae; antimicrobial activity; plumbagin; aliphatic compounds


DRUG SUSCEPTIBILITY

Antimicrobial activity and chemical investigation of Brazilian Drosera

Dalva Trevisan FerreiraI,1 1 Corresponding author. Fax: +55-043-3371.4286. E-mail: dalva@uel.br ; César Cornélio AndreiI; Halha Ostrensky SaridakisII; Terezinha de Jesus FariaI; Elisângela VinhatoI; Kátia Eliane CarvalhoI; Juliana Feijó Souza DanielI; Sílvio Luiz MachadoI; Dennis Panayotis SaridakisI; Raimundo Braz-FilhoIII

IDepartamento de Química, Centro de Ciências Exatas, Universidade Estadual de Londrina, Caixa Postal 6001, 86051-970 Londrina, PR, Brasil

IIDepartamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual do Norte Fluminense, Campos, RJ, Brasil

IIISetor de Química de Produtos Naturais, Universidade Estadual do Norte Fluminense, Campos, RJ, Brasil

ABSTRACT

The antimicrobial activity of three different extracts (hexanic, ethyl acetate, methanol) obtained from Brazilian Drosera species (D. communis, D. montana var. montana, D. brevifolia, D. villosa var. graomogolensis, D. villosa var. villosa, Drosera sp. 1, and Drosera sp. 2 ) were tested against Staphylococcus aureus (ATCC 25923), Enterococcus faecium (ATCC23212), Pseudomonas aeruginosa (ATCC27853), Escherichia coli (ATCC11229), Salmonella choleraesuis (ATCC10708), Klebsiella pneumoniae (ATCC13883), and Candida albicans (a human isolate). Better antimicrobial activity was observed with D. communis and D. montana var. montana ethyl acetate extracts. Phytochemical analyses from D. communis, D. montana var. montana and D. brevifolia yielded 5-hydroxy-2-methyl-1,4-naphthoquinone (plumbagin); long chain aliphatic hydrocarbons were isolated from D. communis and from D. villosa var. villosa, a mixture of long chain aliphatic alcohols and carboxylic acids, was isolated from D. communis and 3b-O-acetylaleuritolic acid from D. villosa var. villosa.

Key words:Drosera species - Droseraceae - antimicrobial activity - plumbagin - aliphatic compounds

There is evidence of the medicinal use of various plants since pre-history (Cowan 1999). The medicinal use of Drosera sp. extracts has been mentioned in the literature since the XVI century as an important antitussive for different respiratory diseases, including tuberculosis (Schnell 1984). The antimicrobial activity of extracts of aerial parts of D. peltata against oral bacteria has also been investigated (Didry et al. 1998). Tinctures prepared with D. rotundifolia leaves may also be used for the treatment of various respiratory diseases (Blumenthal et al. 1998). Since the advent of antibiotics, in the fifties, the use of plant derivatives as antimicrobials has been very limited. However, in recent years, the upsurge of bacterial resistance to antimicrobial drugs has stimulated the search for new drugs of plant origin. The worldwide emergence of Haemophilus, Klebsiella, Escherichia coli, and many other b-lactamase producers became a major therapeutic problem. Methicillin-resistant strains of Staphylococcus aureus are widely distributed in hospitals and are increasingly being isolated from community-acquired infectious strains. Vancomycin-resistant Enterococci and Ente-robacter spp. resistant to most of the known drugs including quinolones, macrolides, cell wall inhibitors, have all increased dramatically over the past decade. Given the evidence for the rapid global spread of resistant clinical isolates and the appearance of drug resistant strains among community acquired infections, the need for discovery or development of new antimicrobial agents active towards these resistant strains is of paramount importance (Bradford 2001). For this reason the search for new antimicrobial drugs among plants became an important alternative. As part of our investigation on the antibacterial properties of plants, a phytochemical study based on the bioactivity screening of five different crude extracts from each of the eight Brazilian Drosera species, was undertaken.

MATERIALS AND METHODS

Plant material - The carnivorous plant species were collected in the region of Ponta Grossa, in the state of Paraná, Southern Brazil, and identified by specialists. Voucher specimens of those species with medicinal use were deposited in the Herbarium of the State University of Londrina.

Preparation of the extracts - The Drosera species: D. communis (2.32 g); D. montana var. montana (2.33 g); D. brevifolia (2.30 g); D. villosa var. graomogolensis (2.30 g) D. villosa var. villosa (2.33 g); D. sp 1 (2.32 g); D. sp 2 (2.32 g), and D. capillaris (2.33 g) were washed under tap water to remove the soil. Each species was extracted with hexane, ethyl acetate, and methanol and the extracts were concentrated in a rotavapor.

Susceptibility testing - Tests were performed by adding 0.5 ml of each plant extract to 15 ml of melted and cooled Muller Hinton Agar (MHA) and poured into Petri dishes (pour plate technique). Young bacterial cultures (log phase) in Muller Hinton Broth, at 36ºC, were streaked with a standardized loop (10 µl) on MHA with incorporated plant extracts and incubated over-night at 36ºC. Growth inhibition was observed after 18 h compared to bacterial growth in MHA without plant extracts.

Phytochemical analysis - The organic extracts of D. communis (methylene chloride), D. montana var. montana (ethyl acetate and methanol), D. brevifolia (hexane), and D. villosa var. villosa (ethyl acetate) were submitted to silica gel column chromatography and eluted with solvents of increasing polarity. The isolated compounds had their structures elucidated by one and two dimensional 1H and 13C NMR, IR, GC, and GC-MS. The compounds isolated and/or identified were: 5-hydroxy-2-methyl-1,4-naphthoquinone (1, plumbagin) from D. communis, D. montana var. montana, and D. brevifolia; long chain aliphatic hydrocarbons in D. communis and D. villosa var. villosa; a mixture of long chain aliphatic alcohols and carboxylic acids in D. communi; and 3b-O-acetylaleuritolic acid (2) in D. villosa var. villosa, this last previously described as an active agent against S. aureus (Peres et al. 1997). The structures of 1 and 2 were confirmed by comparison of the 13C NMR spectral data with values described in the literature for plumbagin (Sankaram et al. 1986) and 3b-O-acetylaleuritolic acid (Ahmad & Atta-ur-Rahman 1994).

RESULTS AND DISCUSSION

Best results were obtained with ethyl acetate extracts from D. montana var. montana and D. communis, as shown in the Table. With the exception of K. pneumoniae (sensitive), the other gram-negative bacteria were resistant to all extracts (P. aeruginosa, E. coli, S. choleraesuis). Among gram-positive bacteria, S. aureus was susceptible at least to one and up to three extracts (hexane, ethyl acetate, and methanol). C. albicans, a yeast, was also susceptible to at least one extract of the species. We consider these as preliminary results and a larger number of bacterial isolates must be tested, with different concentrations of plant extracts in order to establish their real antimicrobial activity. Another feature to be determined is the relation between extract concentration and bacteriostatic or bactericidal effect. The isolation of the naphthoquinone plumbagin (1), from D. communis, D. montana var. montana, and D. brevifolia, together with the non detection of this naphthoquinone in D. villosa var. villosa, are in agreement with the tests accomplished in this study and with the studies on the antimicrobial activity of hydroxy-naphthoquinones described in the literature (Didry et al. 1998). The antimicrobial activity of D. villosa var. villosa towards S. aureus is consistent with the presence of 3b-O-acetylaleuritolic acid as described in the literature (Peres et al. 1997 ).

Plumbagin and 3b-O-acetylaleuritolic acid, isolated from Drosera spp.

Received 19 April 2004

Accepted 15 September 2004

Financial support: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro

++CNPq fellowship

  • Ahmad VU, Atta-ur-Rahman 1994. Handbook of Natural Products Data Pentacyclic Triterpenoids, Vol. 2, Elsevier, Amsterdam, 541 pp.
  • Blumenthal M, Klein J, Hall T 1998. The Complete German Commission E. Monographs, Therapeutic Guide to Herbal Medicines, American Botanical Council, Boston, 685 pp.
  • Bradford PA 2001 Extended-spectrum b-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 14: 933-951.
  • Cowan MM 1999. Plant products as antimicrobial agents. Clin Microbio Rev 12: 564-582.
  • Didry N, Dubreuil L, Trotin F, Pinkas M 1998. Antimicrobial activity of aerial parts of Drosera peltata Smith on oral bacteria. J Ethnopharmacol 60: 91-96.
  • Peres MTLP, Monache FD, Cruz AB, Pizzolatti MG, Yunes RA 1997. Chemical composition and antimicrobial activ-ity of Croton urucurana Baillon (Euphorbiaceae). J Ethnopharmacol 56: 223-226.
  • Sankaram AVB, Reddy VVN, Marthandamurthi M 1986. 13CNMR Spectra of some naturally occurring binaphtho-quinones and related compounds. Phytochemistry 25: 2867-2871.
  • Schnell D 1984. Medicinal uses of some American carnivorous plants. Carniv Plant Newsl 13: 34-36.

  • 1
    Corresponding author. Fax: +55-043-3371.4286. E-mail:

Publication Dates

  • Publication in this collection
    13 Jan 2005
  • Date of issue
    Nov 2004

History

  • Accepted
    Sept 2004
  • Received
    19 Apr 2004
Instituto Oswaldo Cruz, Ministério da Saúde Av. Brasil, 4365 - Pavilhão Mourisco, Manguinhos, 21040-900 Rio de Janeiro RJ Brazil, Tel.: (55 21) 2562-1222, Fax: (55 21) 2562 1220 - Rio de Janeiro - RJ - Brazil
E-mail: memorias@fiocruz.br