Three new species of trypanosomatids were isolated from two species of flies: Herpetomonas anglusteri n. ap., from Liopygia ruficomis (Diptera: Sarcophagidae); Crithidia roitmani n. sp. and Crithidia desouzai n. s.p., from Ornidia obesa (Diptera: Syrphidae). All were axenically cultivated in both complex and defined media and cloned. Giemsa stained preparations showed typical choanomastigotes for Crithidia and promastigotes, paramastigotes, and opisthomastigotes for Herpetomonas. H. anglusteri did grow in a complex medium at 28 and 37ºC, but in a defiend mkedium only at 28ºC. c. roitmani does not grow in coconut's water but C. desouzai grow. Both Crithidia do not require hemin, adenine, and any vitamins and amino acids of the defined medium used in the assay, which suggests that these trypanosomatids may bear endosymbionts.
isolation; trypanosomatids; endosymbiont; nutritional study; Herpetomonas anglusteri n. sp.; Crithidia roitmani n. sp.; Crithidia desouzai n. sp.