The radiopacity of CAC-based compositions containing additives (ZnO, Ag and Bi2O3) was evaluated by optical density measurements, cold crushing strength, apparent porosity, pore size distribution, in vitro apatite-formation ability (bioactivity) and cell viability. The bioactivity was assessed via pH and calcium concentration measurements in simulated body fluid (SBF) for samples previously treated or not with sodium silicate (SS) solution. The surface of the samples after treatment was analyzed by scanning electron microscopy, energy dispersive X-ray analysis and confocal Raman spectroscopy. Cell viability was evaluated by MTT and alkaline phosphatase (ALP) activity. Radiopacity results confirmed that compositions containing ZnO-Ag (25:1 wt%) and ZnO-Bi2O3 (15:10 wt%) presented suitable values of radiopacity (> 3 mmAl). The identification of calcium and mainly phosphorus by EDX, highlighted the generation of an apatite-like layer on the compositions' surface after immersion in SBF or SBF/SS. CAC-blend containing 25 wt% ZnO, ZnO-Ag and ZnO-Bi2O3 presented slightly lower cell viability compared to the positive control.
Keywords:
calcium aluminate blends; bioactivity; osteoblastic cell viability; radiopacity
