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Lethal concentration of methyl methanesulfonate in a new potential invertebrate model for ecotoxicology

Abstract

Sensitivity to different concentrations (0-200 mgL-1) of the alkylating agent methyl methanesulfonate (MMS) was evaluated in the new potential invertebrate model, the amphipod Quadrivisio lutzi (Shoemaker, 1933) after 48 h of exposure in two separate bioassays. In the first bioassay (0, 50, 100 and 200 mgL-1 MMS), all amphipods at concentrations ≥ 50 mgL-1 MMS were dead before completing the 48 h of exposure, which was also observed in the second bioassay for concentrations ≥ 30 mgL-1 MMS. There was no significant effect of gender on average mortality. The median 48 h lethal concentration (LC50) was 23.6 mgL-1 MMS for Q. lutzi. Therefore, 1/3 or less of this concentration may be used for positive control in sublethal conditions. The high sensitivity of Q. lutzi to MMS is discussed in comparison to other invertebrates.

Key Words
Ecotoxicology; Amphipoda; Quadrivisio lutzi; Alkylating agent; Southeastern Brazil

Sociedade Brasileira de Carcinologia Instituto de Biociências, UNESP, Campus Botucatu, Rua Professor Doutor Antônio Celso Wagner Zanin, 250 , Botucatu, SP, 18618-689 - Botucatu - SP - Brazil
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