Expression of self-incompatibility in <i>Coffea canephora</i> genotypes grown in the western Amazon

Depolo, Rodrigo Prado; Rocha, Rodrigo Barros; Souza, Carolina Augusto de; Santos, Maurício Reginaldo Alves dos; Espindula, Marcelo Curitiba; Teixeira, Alexsandro Lara

doi:10.1590/S1678-3921.pab2022.v57.03031

Rodrigo Prado Depolo

Universidade Federal de Rondônia, Programa de Desenvolvimento Regional e Meio Ambiente, Avenida Presidente Dutra, nº 2.965, Olaria, CEP 76801-058 Porto Velho, RO, Brazil. E-mail: rodrigopradodepolo@gmail.com

http://orcid.org/0000-0003-2276-301X

Rodrigo Barros Rocha Corresponding author

Embrapa Rondônia, BR-364, Km 5,5, Caixa Postal 127, CEP 76815-800 Porto Velho, RO, Brazil. E-mail: rodrigo.rocha@embrapa.br, mauricio.santos@embrapa.br, marcelo.espindula@embrapa.br, alexsandro.teixeira@embrapa.br

http://orcid.org/0000-0001-5275-5315

Carolina Augusto de Souza

Universidade Federal de Rondônia, Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Avenida Presidente Dutra, nº 2.965, Olaria, CEP 76801-058 Porto Velho, RO, Brazil. E-mail: carolina_augusto@hotmail.com

http://orcid.org/0000-0003-3557-5499

Maurício Reginaldo Alves dos Santos

Embrapa Rondônia, BR-364, Km 5,5, Caixa Postal 127, CEP 76815-800 Porto Velho, RO, Brazil. E-mail: rodrigo.rocha@embrapa.br, mauricio.santos@embrapa.br, marcelo.espindula@embrapa.br, alexsandro.teixeira@embrapa.br

http://orcid.org/0000-0002-0451-7299

Marcelo Curitiba Espindula

Embrapa Rondônia, BR-364, Km 5,5, Caixa Postal 127, CEP 76815-800 Porto Velho, RO, Brazil. E-mail: rodrigo.rocha@embrapa.br, mauricio.santos@embrapa.br, marcelo.espindula@embrapa.br, alexsandro.teixeira@embrapa.br

http://orcid.org/0000-0001-7481-9746

Alexsandro Lara Teixeira

Embrapa Rondônia, BR-364, Km 5,5, Caixa Postal 127, CEP 76815-800 Porto Velho, RO, Brazil. E-mail: rodrigo.rocha@embrapa.br, mauricio.santos@embrapa.br, marcelo.espindula@embrapa.br, alexsandro.teixeira@embrapa.br

http://orcid.org/0000-0003-1125-7082

Corresponding author

Figures (2)
Tables (4)

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Figure 1
Visualization under a microscope at 50X magnification, showing: A, pollinated stigma without the development of pollen tubes; and B, pollination of only one of the sides of the bifid stigma, which favors the visualization under the microscope due to the contrast between the pollinated and nonpollinated styles – pollen tubes can be seen growing through the style tissue in the compatible hibridization.

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Figure 2
Scatter plot of the first two principal components of 62 Coffea canephora genotypes of the conilon and robusta botanical varieties, which are identified by geometric shapes (triangle, conilon; and square, robusta). The letters A, B, C, and D identify the quadrants of greatest similarity, and the centroids represent the plants with the typical characteristics of the conilon botanical variety (centroid 1) and robusta botanical variety (centroid 2). The Roman numerals represent the compatibility groups.

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Table 1
Hybridizations performed for 62 Coffea canephora genotypes with test plants of compatibility groups I, II, and III in the municipality of Porto Velho, in the state of Rondônia, Brazil⁽¹⁾.

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Table 2
Hybridizations in a complete diallel design with self-fertilizations and reciprocal crosses of six Coffea canephora genotypes, with characteristics of the conilon and robusta botanical varieties that were compatible with all the previously known testers, showing: A, compatibility response of the directed hybridizations (NC, noncompatible; and C, compatible); and B, grouping of new compatibility groups designated as IV, V, and VI. Genotypes: C, plants of the conilon botanical variety; and R, plants of the robusta botanical variety.

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Table 3
Hybridizations performed of 32 Coffea canephora genotypes with test plants of compatibility groups IV, V, and VI in the municipality of Porto Velho, in the state of Rondônia, Brazil⁽¹⁾.

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Table 4
Grouping of 62 parent plants of Coffea canephora of the conilon and robusta botanical varieties, identified in their respective groups in accordance with the compatibility with test plants in the municipality of Porto Velho, in the state of Rondônia, Brazil⁽¹⁾.

n	Genotype	I	II	III	FN	Group	p-value	n	Genotype	I	II	III	FN	Group	p-value
1	C 167	(2)	1	1	0	I	<0.01	32	R 216	(2)	1	1	1	I	<0.01
2	C 799	(2)	1	1	0	I	<0.01	33	R 3	(2)	1	2	0	I	<0.01
3	C 986	(2)	1	1	0	I	<0.01	34	R 277	1	(2)	1	0	II	<0.01
4	C 154	(2)	1	1	0	I	<0.01	35	R 63	1	(2)	1	0	II	<0.01
5	C 452	(2)	1	1	0	I	<0.01	36	R 9	1	(2)	1	0	II	<0.01
6	C468	1	(2)	2	0	II	<0.01	37	R 99	3	(2)	2	0	II	<0.01
7	C 795	1	(2)	1	1	II	<0.01	38	R 164	2	2	(2)	0	III	<0.01
8	C 945	1	(2)	1	0	II	<0.01	39	R 32	3	1	(2)	0	III	<0.01
9	C 125	2	(1)	2	0	II	0.05	40	R 36	2	1	(3)	0	III	<0.01
10	C 184	1	(1)	1	0	II	0.05	41	R 20	3	2	3	0	New	-
11	C 59	1	(2)	1	0	II	<0.01	42	R 161	1	1	1	0	New	-
12	C 729	1	(1)	2	0	II	0.05	43	R 186	1	3	2	0	New	-
13	C 801	1	(2)	1	0	II	<0.01	44	R 222	2	1	1	0	New	-
14	C 46	1	(2)	2	0	II	<0.01	45	R 240	2	1	2	0	New	-
15	C 484	1	1	(2)	0	III	<0.01	46	R 302	1	1	2	1	New	-
16	C 968	1	1	(1)	0	III	0.05	47	R 212	1	2	1	0	New	-
17	C 694	2	1	(2)	0	III	<0.01	48	R 224	2	2	2	1	New	-
18	C 796	1	1	1	0	New	-	49	R 157	1	2	1	0	New	-
19	C 998	1	1	1	0	New	-	50	R 160	1	2	1	1	New	-
20	C 1048	2	3	3	1	New	-	51	R 220	1	3	1	0	New	-
21	C 1059	2	2	1	1	New	-	52	R 243	1	6	1	0	New	-
22	C 556	2	1	1	1	New	-	53	R 307	2	1	1	2	New	-
23	C 747	1	1	1	0	New	-	54	R 309	1	1	1	0	New	-
24	C 846	1	1	1	0	New	-	55	R 312	2	4	2	0	New	-
25	C 890	1	1	1	0	New	-	56	R 42	3	3	2	0	New	-
26	C 909	1	1	1	0	New	-	57	R 66	1	4	1	0	New	-
27	C 973	1	1	1	0	New	-	58	R 90	1	1	2	1	New	-
28	R 183	(2)	2	1	1	I	<0.01	59	R 1	1	2	1	0	New	-
29	R 30	(1)	4	1	0	I	0.05	60	R 131	2	4	2	0	New	-
30	R 5	(2)	1	1	0	I	<0.01	61	R 125	1	3	2	1	New	-
31	R 214	(2)	1	1	0	I	<0.01	62	R 155	1	2	1	0	New	-

A
Genotypes	C1059	C556	C1048	R309	R161	R224
C1059	NC¹	NC	C	C	C	C
C556	NC²	NC¹	C	C	C	C
C1048	C2	C2	NC¹	C	NC	NC
R309	C²	C²	C²	NC¹	C	C
R161	C²	C²	NC²	C²	NC¹	NC
R224	C²	C²	NC²	C²	NC²	NC¹

B
Groups	IV	V	VI
IV	C556	R309	R161
V	C1059		R224
VI			C1048

n	Clone	IV	V	VI	FN	Group	p-value
1	C 1048	4	3	(3)	0	VI	<0.01
2	C 1059	(3)	4	1	1	IV	<0.01
3	C 556	(3)	4	2	0	IV	<0.01
4	C 747	(2)	1	2	0	IV	<0.01
5	C 973	2	(1)	1	1	V	0.05
6	C 796	(1)	1	1	1	IV	0.05
7	C 846	4	3	(2)	0	VI	<0.01
8	C 890	2	(2)	1	0	V	<0.01
9	C 909	2	(2)	2	0	V	<0.01
10	C 998	2	(2)	2	0	V	<0.01
11	R 90	1	1	(1)	1	VI	0.05
12	R 1	(2)	1	2	0	IV	<0.01
13	R 125	1	1	(2)	0	VI	<0.01
14	R 131	1	1	(2)	0	VI	<0.01
15	R 155	2	1	(2)	1	VI	<0.01
16	R 157	1	1	(2)	0	VI	<0.01
17	R 160	1	2	(2)	0	VI	<0.01
18	R 161	1	1	(1)	0	VI	0.05
19	R 186	(2)	1	1	0	IV	<0.01
20	R 20	2	1	(2)	0	VI	<0.01
21	R 212	1	1	(2)	0	VI	<0.01
22	R 220	1	1	(2)	0	VI	<0.01
23	R 222	1	1	(1)	0	VI	0.05
24	R 224	1	1	(1)	0	VI	0.05
25	R 240	1	1	(1)	0	VI	0.05
26	R 243	(2)	2	1	0	IV	<0.01
27	R 302	2	(2)	1	1	V	<0.01
28	R 307	1	(1)	1	0	V	0.05
29	R 309	1	(2)	1	0	V	<0.01
30	R 312	1	(2)	1	0	V	<0.01
31	R 42	(1)	1	1	0	IV	0.05
32	R 66	(2)	2	1	0	IV	<0.01

n	I	II	III	IV	V	VI
n	S₁S₂	S₁S₃	S₂S₃	S_(1,2,3)S₄	S_(1,2,3)S₄	S_(1,2,3)S₄
1	C 154	C 125	C 484	C 1059	C 890	C 1048
2	C 167	C 184	C 694	C 556	C 909	C 846
3	C 452	C 46	C 968	C 747	C 998	R 90
4	C 799	C468	R 164	C 796	C 973	R 160
5	C 986	C 59	R 32	R 243	R 309	R 222
6	R 183	C 795	R 36	R 186	R 312	R 20
7	R 214	C 801	-	R 1	R 302	R 161
8	R 216	C 945	-	R 42	R 307	R 131
9	R 3	C 729	-	R 66	-	R 125
10	R 30	R 63	-	-	-	R 155
11	R 5	R 9	-	-	-	R 157
12	-	R 99	-	-	-	R 224
13	-	R 277	-	-	-	R 212
14	-	-	-	-	-	R 220
15	-	-	-	-	-	R 240
Total	11	13	6	9	8	15
Conilon	5	9	3	4	4	2
Robusta	6	4	3	5	4	13
Robusta (%)	54.5	30.8	50.0	55.6	50.0	86.7

[1] Corresponding author

Brasil

Brasil

Expression of self-incompatibility in Coffea canephora genotypes grown in the western Amazon

Expressão da autoincompatibilidade em genótipos de Coffea canephora cultivados na Amazônia Ocidental

Abstract