Bactérias endofíticas promotoras de crescimento de plantas em mudas de pupunheira

Silva, Krisle da; Quisen, Regina Caetano; Goldbach, Juliana Degenhardt; Pepe, Kauanna Brok Ferreira; Kalil Filho, Antônio Nascim

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Pesquisa Agropecuária Brasileira

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Sumário

Forestry, Original Article • Pesq. agropec. bras. 57 • 2022 • https://doi.org/10.1590/S1678-3921.pab2022.v57.02962 copiar

Bactérias endofíticas promotoras de crescimento de plantas em mudas de pupunheira

Autoria SCIMAGO INSTITUTIONS RANKINGS

Resumo

O objetivo deste trabalho foi isolar bactérias endofíticas de pupunheira (Bactris gasipaes var. gasipaes) e avaliar os efeitos da inoculação delas em mudas da planta. As bactérias foram isoladas de folhas e raízes das mudas e de meristemas de pupunheira in vitro. Os isolados foram caracterizados fenotipicamente, e, depois, 15 deles, representando grupos fenotípicos distintos, foram selecionados e identificados por meio do sequenciamento parcial do gene 16S rRNA. Em seguida, esses isolados e duas estirpes comerciais de Azospirillum brasilense (Ab-V5 e Ab-V6) foram inoculados em plântulas de pupunheira. Após 76 dias, as mudas foram avaliadas quanto ao desenvolvimento vegetal. Foram identificados os seis seguintes gêneros com base no sequenciamento: Pseudomonas, Enterobacter, Rhizobium, Stenotrophomonas, Klebsiella e Erwinia. Dos 15 isolados inoculados, 9 tiveram efeito positivo sobre a massa de matéria seca de raízes, com destaque para CNPF 77 (Enterobacter sp.), CNPF 100 (Rhizobium sp.), e CNP 179 e CNPF 277 (Stenotrophomonas sp.). Mudas de pupunheira abrigam bactérias endofíticas capazes de aumentar a matéria seca das raízes.

Termos para indexação:
Bactris gasipaes var. gasipaes; Enterobacter ; Rhizobium ; Stenotrophomonas ; bioinoculantes; 16S rRNA

Isolate	Origin	Morphological characteristics	Genus	Genbank number
CNPF 77	Roots	Very fast growth, circular colony, convex, entire margin, rugose surface, moderate mucus, translucent, cream color, and homogenous colonies.	Enterobacter sp.	OM912659
CNPF 90	Meristems	Fast growth, circular colony, convex, entire margin, smooth surface, moderate mucus, translucent, white color, and homogenous colonies.	Klebsiella sp.	OM912658
CNPF 94	Leaves	Fast growth, circular colony, raised, entire margin, smooth surface, abundant mucus, translucent, cream color, and homogenous colonies.	Rhizobium sp.	OM912657
CNPF 99	Roots	Very fast growth, circular colony, fat, erose margin, rugose surface, little mucus, transparent, cream color, and heterogenous colonies.	Pseudomonas sp.	OM912656
CNPF 100	Leaves	Fast growth, circular colonies, raised, entire margin, smooth surface, moderate mucus, translucent, cream color and homogenous colonies.	Rhizobium sp.	OM912655
CNPF 105	Meristems	Intermediary growth, circular colonies, convex, entire margin, smooth surface, moderate mucus, opaque, black color, and homogenous colonies.	Klebsiella sp.	OM912654
CNPF 108	Roots	Fast growth, circular colonies, convex, entire margin, smooth surface, moderate mucus, translucent, cream color, and heterogenous colonies.	Enterobacter sp.	OM912653
CNPF 110	Meristems	Fast growth, circular colonies, convex, entire margin, smooth surface, abundant mucus, opaque, cream color, and homogenous colonies.	Erwinia sp.	OM912652
CNPF 118	Leaves	Very fast growth, circular colonies, fat, erose margin, rugose surface, moderate mucus transparent, cream color, and homogenous colonies.	Pseudomonas sp.	OM912651
CNPF 152	Roots	Fast growth, circular colonies, raised, undulate margin, smooth surface, moderate mucus, translucent, cream color, and homogenous colonies.	Rhizobium sp.	OM912650
CNPF 154	Leaves	Fast growth, irregular colonies, fat, lobate margin, rugose surface, moderate mucus, translucent, yellow and cream colors, and heterogenous colonies.	Pseudomonas sp.	OM912649
CNPF 179	Roots	Fast growth, circular colonies, fat, entire margin, smooth surface, sparse mucus, translucent, cream color, and homogenous colonies.	Stenotrophomonas sp.	OM912648
CNPF 235	Leaves	Fast growth, circular colonies, convex, erose margin, smooth surface, moderate mucus, transparent, yellow and cream colors, and heterogenous colonies.	Enterobacter sp.	OM912647
CNPF 248	Leaves	Fast growth, circular colonies, convex, entire margin, smooth surface, abundant mucus, translucent, cream color, and homogenous colonies.	Enterobacter sp.	OM912646
CNPF 277	Leaves	Fast growth, irregular colonies, fat, lobate margin, smooth surface, moderate mucus, translucent, yellow and cream colors, and heterogenous colonies.	Stenotrophomonas sp.	OM912645

Treatment	SDM	RDM	RDM/SDM	Plant height (cm per plant)	Number of leaves (no. per plant)
Treatment	(g per plant)		RDM/SDM	Plant height (cm per plant)	Number of leaves (no. per plant)
CNPF 77 (Enterobacter sp.)	5.68	6.57a	1.18a	13.60	4.60
CNPF 90 (Klebsiella sp.)	5.44	5.53b	1.02b	14.40	4.20
CNPF 94 (Rhizobium sp.)	4.94	5.63b	1.15a	12.75	4.25
CNPF 99 (Pseudomonas sp.)	5.40	5.07c	0.93b	11.75	3.75
CNPF 100 (Rhizobium sp.)	5.45	6.73a	1.25a	13.60	3.80
CNPF 105 (Klebsiella sp.)	5.15	5.51b	1.06a	14.00	3.75
CNPF 108 (Enterobacter sp.)	4.58	4.36c	0.95b	13.00	4.25
CNPF 110 (Erwinia sp.)	5.61	4.94c	0.90b	12.20	4.20
CNPF 118 (Pseudomonas sp.)	5.06	5.50b	1.10a	13.00	4.50
CNPF 152 (Rhizobium sp.)	4.43	5.68b	1.29a	13.25	4.25
CNPF 154 (Pseudomonas sp.)	4.08	4.22c	1.05a	13.00	4.00
CNPF 179 (Stenotrophomonas sp.)	6.31	7.03a	1.18a	12.40	4.20
CNPF 235 (Enterobacter sp.)	4.50	4.26c	0.94b	13.40	4.20
CNPF 248 (Enterobacter sp.)	5.40	4.17c	0.77b	13.60	4.40
CNPF 277 (Stenotrophomonas sp.)	5.23	6.43a	1.25a	13.00	4.20
Ab-V5 (Azospirillum brasilense)	5.05	4.71c	0.93b	13.25	4.34
Abv-V6 (Azospirillum brasilense)	5.29	4.81c	0.91b	12.80	4.20
Ab-V5 + Abv-V6	5.17	5.42b	1.09a	12.60	4.00
Control without inoculation	4.81	4.52c	0.91b	13.00	4.00
Coefficient of variation (%)	17.85	20.97	5.14	14.16	5.86

Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira Caixa Postal 040315, 70770-901 Brasília DF Brazil, Tel. +55 61 3448-1813, Fax +55 61 3340-5483 - Brasília - DF - Brazil
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