Validation of molecular markers associated to leaf rust resistance genes in wheat

The objective of this work was to validate molecular markers previously associated to leaf rust resistance genes in Brazilian wheat genotypes. Five STS and SCAR markers identified as associated to the resistance alleles of the genes Lr1, Lr9, Lr10 and Lr24 were tested by PCR in 25 Brazilian wheat genotypes with known presence or absence of these alleles. The STS marker associated to Lr1 was not efficient in identifying Brazilian genotypes carrying its resistance allele. The STS and SCAR markers associated to Lr9, Lr10 and Lr24 are efficient in identifying plants carrying the resistance alleles for these genes, and therefore, can be used for marker-assisted selection of leaf rust resistance in Brazilian wheat genotypes.

Puccinia triticina; Triticum aestivum; SCAR; STS

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