Figure 1
Type-locality of Spirobranchus lirianeae sp. nov. (A) Rio de Janeiro state location at Brazilian coast, (B) detail of Rio de Janeiro state and sample area (green square) (C) sampled locations (stars) at Ilha Grande bay. Scale bars = 250 km (A), 75 km (B), 2,5 km (C).
Figure 2
Spirobranchus lirianeae sp. nov. (A-C) Holotype (MNRJP-002774); (D-E, H) Paratype (MNRJP-002775); (F-G) Paratype (MNRJP 002777). (A) entire worm, ventral view, (B) dorsal view, (C) anterior end of tube, with exposed operculum, collar lobes and crown, (D) bases of radioles, with paired compound ocellar clusters, (E) detail of a ocellar cluster, (F) operculum, dorsal view, (G) operculum clarified with glycerin, exposing talon, (H) internal crown, with folded mid-ventral lobe and dorsal lip. Scale bars = 1 mm (A, B, C), 0,2 mm (D, F, G), 50 µm (H), 3 µm (E). A = abdomen; a = apron; c = collar; dl = dorsal lip; ml = mid-ventral lobe; o = operculum; p = peduncle; RC = radiolar crown; t = talon; T = thorax; w = wings. Arrows pointing compound ocellar clusters.
Figure 3
Spirobranchus lirianeae sp. nov. (A, C, D) (righ hand image), Paratype (MNRJP-002775); (B, D) (left hand image), (E-G) Holotype (MNRJP-002774). (A) anterior end, superior view, (B) thorax, lateral view, (C) anterior end, dorsal view, showing collar dorso-lateral lobes (D) anterior end, through beginning of abdomen, ventral view, stained with methylene blue, (E) anterior abdominal chaetigers, lateral view, (F) posterior end, ventro-lateral view, (G) posterior end, ventral view, stained with methylene blue. Scale bars = 0,2 mm (A, B, C, D, E, F, G). a = apron; dl = dorso-lateral lobe; ml = mid-ventral lobe; o = operculum; t = tonguelet; tm = thoracic membrane; w = wing. Arrows pointing glandular regions.
Figure 4
SEM images of Spirobranchus lirianeae sp. nov. Paratype (MNRJP-002776). (A) entire worm, lateral view, (B) crown and operculum, ventral view, (C) detail of operculum, (D) thoracic limbate notochaetae, (E) thoracic uncini, (F) abdominal uncini, (G) abdominal neurochaetae. Scale bars = 500 µm (A), 200 µm (B, C), 20 µm (D), 10 µm (E), 5 µm (F, G). t = tonguelet; w = wing.
Figure 5
Spirobranchus giganteus (MNRJP-002778). (A) entire worm, inside fragment of tube, lateral view, (B) anterior end of tube, (C) operculum and radiolar crown, dorsal view, (D) same as C, ventral view, (E) operculum, superior view, (F) base of crown, exposing oral palp, (G) thorax, ventral view, stained with methylene blue, (H) thoracic notopodia, (I) anterior abdominal chaetigers Scale bars = 1 mm (A, B, C, D, E, G), 0,3 mm (I), 0,2 mm (H), 0,1 mm (F). lk = longitudinal keel. Arrows pointing the oral palp (F) and parapodial cirri (H).
Figure 6
Spirobranchus minutus. (A) MNRJP-002780; (B-C, E) MNRJP-002783; (D, F, G) MNRJP-002785. (A) entire worm, dorsal view, (B) tube, (C) anterior end, dorsal view, (D) operculum, superior view, (E) operculum in juvenile, dorsal view, (F) thorax (except collar), ventral view, stained with methylene blue, (G) anterior abdominal chaetigers, lateral view, stained with methylene blue. Scale bars = 1 mm (A), 500 µm (B), 200 µm (C), 100 µm (F, G), 50 µm (D, E). t = tonguelet; w = wing.
Figure 7
Spirobranchus tetraceros. (A, D-E, J) MNRJP-002788; (B-C, F-I) MNRJP-002789. (A) anterior end of tube with exposed operculum, (B) anterior end through anterior abdomen of specimen with ‘tetraceros’ condition, ventral view, (C) radioles and interradiolar membrane, (D) conical ‘tricornis’ operculum, (E) ‘tricornis’ opercular condition, (F, G) peduncular wings, in a ‘tetraceros’ operculum, (F) dorsal view, (G) ventral view, (H) anterior end, from base of crown through anterior abdomen, lateral view, (I) inner side of crown and dorsal lip, (J) thorax (except collar), ventral view, stained with methylene blue, (K) same, lateral view. Scale bars = 1 mm (A, B), 0,4 mm (D, E), 0,5 mm (F, G, H, I, J, K), 0,2 mm (C). d = dorsal lip; ip = inter-radiolar processes; o = operculum; t = tonguelets; w = wing.
Figure 8
SEM images of chaetae from species of Spirobranchus reported in Brazil. (A-G) S. giganteus (MNRJP-002779): (A) collar chaetae, (B) detail of shaft of Spirobranchus-type chaeta, (C) thoracic notopodia, (D) limbate thoracic notochaetae, (E) thoracic uncini, (F) abdominal uncini, (G) abdominal trumpet-shaped neurochaetae; (H-K) S. minutus (MNRJP-002785): (H) limbate thoracic notochaetae, (I) thoracic uncini, (J) abdominal uncini, (K) abdominal trumpet-shaped neurochaetae (lateral projection broken); (L-Q) S. tetraceros (MNRJP-002789): (L) collar chaetae, (M) thoracic notopodium, (N) detail of limbate thoracic notochaetae, (O) thoracic uncini, (P) abdominal uncini, (Q) abdominal trumpet-shaped neurochaetae. Scale bars = 500 µm (C), 200 µm (A, M), 100 µm (D, L), 20 µm (N, Q), 10 µm (B, E, F, G, H, K, O, P), 5 µm (I), 2 µm (J). lc = limbate chaeta; pc = parapodial cirrus; sc = Spirobranchus-type chaeta.
Figure 9
Tonguelets among members of Spirobranchus spp. (A, B) S. lirianeae sp. nov.: (A) Paratype (MNRJP-002775) peduncle and dorso-lateral collar lobes dissected, (B) Paratype (MNRJP-002777) detail of the bilobed tonguelet; (C, D) S. giganteus (MNRJP-002779): (C) dorsal view, (D) dorsal view with dorsal outer lobe folded backwards, exposing inner lobe; (E, F) S. minutus (MNRJP-002783): (E) lateral view, (F) detail of tonguelet, dorsal view, stained with Shirlastain, with dorso-lateral collar lobe dissected; (G, H) S. tetraceros (MNRJP-002789): (G) lateral view, (H) detail of lateral view stained with methylene blue, outer lobe over inner lobe. Scale bars = 300 µm (G), 200 µm (A, C, D, H), 150 µm (B), 100 µm (E), 50 µm (F). dol = dorsal outer tonguelet lobe; dl = dorsolateral colar lobe; il = inner tonguelet lobe; ol = outer tonguelet lobe; p = peduncle; vol = ventral outer tonguelet lobe.
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
