Effect of Novel Chelating Agents on the Dislodgement Resistance of Biodentine: An Ex-Vivo Study

ABSTRACT

Objective:  To investigate the effect of 0.26mol/L Clodronate Disodium Tetrahydrate, 9% Etidronic acid (HEDP), and 17% Ethylenediaminetetraacetic acid (EDTA) on the dislodgement resistance of Biodentine.

Material and Methods:  80 human single-rooted premolar teeth were selected. For bond strength analysis, samples were treated (n=15) according to the irrigation protocol employed: EDTA Group - 2.5% sodium hypochlorite (NaOCl) + 17% EDTA; HEDP Group - 9% Etidronic acid + 2.5% NaOCl; Clodronate Group- 0.26mol/L Clodronate + 2.5% NaOCl; and Control Group- 2.5% NaOCl + Distilled Water. Root canals were condensed with Biodentine, and 1.5 mm thick dentine discs were produced, and push-out bond strength was analysed. For FTIR analysis, dentine samples (n=5) were prepared (1cm × 1cm). Pre and post-irrigation scans were performed to compare compositional changes in the Amide III, Phosphate, and Carbonate infrared bands. One-way analysis of variance with post-hoc Tukey’s test was employed to analyze the results.

Results:  The highest bond strength value was obtained with 0.26mol/L Clodronate, followed by 9% HEDP and 17% EDTA (p<0.001). Amide III: Phosphate levels decreased with EDTA and HEDP, whereas the opposite was observed with Carbonate: Phosphate. No difference was observed with 0.26 mol/L Clodronate. Fractographic analysis revealed 20% adhesive failure, 48% mixed failure, and 32% adhesive failure, respectively.

Conclusion:  0.26 mol/L Clodronate produced the most favourable outcome in terms of bond strength of Biodentine.

Keywords:
Clodronic Acid; Etidronic Acid; Endodontics; Smear Layer.