Open-access In Vitro Cytotoxicity and Genotoxicity Evaluation of an Orabase Libidibia ferrea L.

ABSTRACT

Objective:  To evaluate the cytotoxic and genotoxic effects of a phytotherapeutic ointment containing 2% L. ferrea L extract.

Material and Methods:  Libidibia ferrea L. barks were used as raw material. The extract was prepared by decoction under reflux in a hydroalcoholic solution. The extract concentration in the orabase formulation was determined based on the minimum inhibitory concentration. Biocompatibility was assessed by cell culture tests from MRC5 human fibroblasts. The Alamar Blue test evaluated cytotoxicity; the Trypan Blue Solution trial evaluated cell viability. The comet assay on alkaline and neutral pH assessed genotoxicity. Test results were tabulated and analyzed through descriptive and inferential statistics, with ANOVA Test (p≤ 0.05).

Results:  No cytotoxic activity or morphological induction of cell death for the test formulation. Regarding genotoxicity, the Juca formulation could not induce DNA damage in MRC-5 at 25 and 50 μg/mL concentrations through the comet assay at neutral or alkaline pH.

Conclusion:  The tested formulation is biocompatible, and no cytotoxic or genotoxic effects were observed under test conditions, demonstrating viability for future in vivo tests.

Keywords:
Phytotherapy; Oral Ulcer; Plant Extracts

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