Intoxication of Mesotrione in Corn Inoculated with <i>Azospirillum brasilense</i> and with Application of Plant Growth Regulators

BULEGON, L.G.; GUIMARÃES, V.F.; BATTISTUS, A.G.; COSTA, N.V.

doi:10.1590/S0100-83582019370100058

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Article • Planta daninha 37 • 2019 • https://doi.org/10.1590/S0100-83582019370100058 copy

Intoxication of Mesotrione in Corn Inoculated with Azospirillum brasilense and with Application of Plant Growth Regulators

Intoxications Mesotrione in maize inoculated with Azospirillum brasilense and with application of Plant Growth Regulator

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT:

The objective of this study was to evaluate the effect of intoxication by mesotrione herbicide on the initial development and yield of maize after seed inoculation or foliar spray application with Azospirillum brasilense and application of plant regulators. Therefore, three experiments were performed: one under greenhouse conditions and two under field conditions, in different locations. In all experiments, a randomized block design was used with a 2 x 4 factorial arrangement, representing presence (192 g ha-1) or absence of mesotrione and four treatments corresponding to the control; seed inoculation with A. brasilense; foliar spray application of A. brasilense; foliar spay application of auxin + gibberellin + cytokine (AX + GA + CK), and foliar spay applications with mesotrione were carried out at the V3 stage of the crop. The results from the greenhouse experiment, with mesotrione application, were intoxication of 7.12%, which reduced total chlorophyll content by 10.15% and carotenoid content by 75.86%, leading to reductions in gas exchange and chlorophyll a fluorescence, and increased activity of antioxidative enzymes. Under field conditions, the treatments did not reduce the effects of intoxication during the initial development of maize crop and did not increase crop yield. It was concluded that the use of A. brasilense and plant regulators did not protect maize against mesotrione intoxication in the initial development, nor did it increase yield.

Keywords:
Zea mays; gas exchange; fluorescence of chlorophyll a; peroxidase; catalase

Sociedade Brasileira da Ciência das Plantas Daninhas Departamento de Fitotecnia - DFT, Universidade Federal de Viçosa - UFV, 36570-000 - Viçosa-MG - Brasil, Tel./Fax::(+55 31) 3899-2611 - Viçosa - MG - Brazil
E-mail: rpdaninha@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] * Corresponding author: <vandeirfg@yahoo.com.br>

Treatment	1 DAA		7 DAA		14 DAA
Treatment	Into (%)	SPAD	Into (%)	SPAD	Into (%)	SPAD
Mesotrione
With Mesotrione	1.93 a	40.46	7.21 a	42.49 b	0.68 a	50.00 b
Without Mesotrione	0.00 b	41.95	0.00 b	48.08 a	0.00 b	52.95 a
Treatment
Control	1.25	40.45	3.87	44.51 b	0.50	52.62
Foliar application of A. brasilense	1.00	40.97	3.31	49.35 a	0.00	50.25
Seed inoculation of A. brasilense	0.87	39.98	3.75	42.36 b	0.37	49.93
AX+GB+CK	0.75	43.41	3.50	44.86 b	0.50	53.07
Calculated F values
Treatment	1.244^ns	1.250^ns	0.256^ns	7.073**	4.013^ns	1.342^ns
Mesotrione	102.442**	1.192^ns	210.122**	25.300**	33.800**	4.504*
Interaction	1.244^ns	2.280^ns	0.256^ns	2.107^ns	4.013^ns	1.439^ns
CV (%)	55.89	9.37	39.02	6.90	97.19	7.61

Treatment	Total chlorophyll (µg g^-1)			Carotenoids (µg g^-1)
Treatment	With Mesotrione	Without Mesotrione	Mean	With Mesotrione	Without Mesotrione	Mean
Control	51.68 aA	47.93 bA	48.81	0.57 abA	0.65 bA	0.61
Foliar application of A. brasilense	49.61 aA	51.47 abA	50.54	0.25 bB	1.44 aA	0.84
Seed inoculation of A. brasilense	39.32 bB	57.06 aA	48.19	0.61 abA	0.90 bA	0.76
AX+GB+CK	47.64 aA	50.89 abA	49.27	0.90 aA	1.10 abA	1.00
Mean	47.06 B	51.84 A		0.58 B	1.02 A
Calculated F values
Treatment	0.540^ns			2.651^ns
Mesotrione	12.541**			19.319**
Interaction	167.59**			6.441**
CV (%)	7.71			35.08

Treatment	CAT 1 DAA UE min^-1 g fresh weight^-1			POD 1 DAA UE min^-1 g fresh weight^-1
	With Mesotrione	Without Mesotrione	Mean	With Mesotrione	Without Mesotrione	Mean
Control	9.48 aA	2.80 aB	6.14	107.09 cA	142.36 abA	124.73 c
Foliar application of A. brasilense	3.51 bA	5.51 aA	4.51	217.49 bA	119.61 bB	168.55 b
A. brasilense seed	3.02 bA	4.59 aA	3.81	143.10 cA	97.57 bA	120.34 c
AX+GB+CK	8.60 aA	4.12 aB	6.36	277.79 aA	197.06 aB	237.43 a
Mean	6.15A	4.26 B		186.37 A	139.15 B
Calculated F values
Treatment	2.837^ns			16.518**
Mesotrione	6.582**			12.478**
Interaction	8.661**			4.895**
CV (%)	40.11			23.23
Treatment	CAT 7 DAA (UE min^-1 g fresh weight^-1)
	With Mesotrione		Without Mesotrione		Mean
Control	6.66 aA		8.10 aA		7.38 a
Foliar application of A. brasilense	8.14 aA		4.00 bB		6.07 a
Seed inoculation of A. brasilense	2.90 bA		4.83 bA		3.86 b
AX+GB+CK	6.01 aA		8.43 aA		7.22 a
Mean	5.93		6.34
Calculated F values
Treatment	6.874**
Mesotrione	0.440^ns
Interaction	6.121**
CV (%)	28.50

Treatment	A (µmol CO₂ m^-2 s^-1)		E (mmol H₂O m^-2 s^-1)		gs (µmol m^-2 s^-1)
With Mesotrione	34.48 b		3.55 b		0.203 b
Without Mesotrione	51.16 a		4.64 a		0.297 a
Calculated F values
Treatment	0.285^ns		0.516^ns		0.703^ns
Mesotrione	30.775**		20.809**		16.221**
Interaction	0.776^ns		0.583^ns		0.808^ns
CV (%)	14.42		16.64		26.28
Treatment	Fo	Fm	Fv/Fm	ՓPSII	Fv’/Fm’	qP
With Mesotrione	117.36	556.55 b	0.788 b	0.284 b	0.464 b	0.612 b
Without Mesotrione	118.47	584.57 a	0.797 a	0.334 a	0.499 a	0.669 a
Calculated F values
Treatment	0.016^ns	2.076^ns	1.559^ns	0.286^ns	0.121^ns	2.343^ns
Mesotrione	0.807^ns	10.034**	4.396*	38.205**	11.476**	42.540**
Interaction	0.360^ns	0.466^ns	0.559^ns	0.224^ns	0.071^ns	0.732^ns
CV (%)	2.96	4.38	1.48	7.38	6.12	3.89

Treatment	Intoxication (%)			SPAD index
Treatment	With Mesotrione	Without Mesotrione	Mean	With Mesotrione	Without Mesotrione	Mean
Control	3.0	0.0	1.5	34.95	42.45	38.70
Foliar application of A. brasilense	2.5	0.0	1.25	37.42	38.42	37.92
Seed inoculation of A. brasilense	2.25	0.0	1.125	37.82	38.75	38.28
AX+GB+CK	3.0	0.0	1.5	40.02	36.40	38.21
Mean	2.68 A	0.0 B		37.55	39.00
Treatment	Total chlorophyll (µg g^-1)			Carotenoids (µg g^-1)
Treatment	With Mesotrione	Without Mesotrione	Mean	With Mesotrione	Without Mesotrione	Mean
Control	39.16 aA	41.58 aA	40.37	1.07 aA	0.70 abA	0.89
Foliar application of A. brasilense	43.11 aA	36.17 aB	39.64	0.37 bB	0.77 abA	0.57
Seed inoculation of A. brasilense	44.66 aA	37.12 aB	40.89	0.73 abA	0.45 bA	0.59
AX+GB+CK	37.23 aA	38.72 aA	37.97	0.67 abB	1.03 aA	0.85
Mean	41.04	38.40		0.71	0.74
Calculated F values
	Intoxication		SPAD		Total chlorophyll	Carotenoids
Treatment	0.582^ns		0.041^ns		0.762^ns	3.617*
Mesotrione	119.474*		0.845^ns		3.297^ns	0.112^ns
Interaction	0.582^ns		2.104^ns		3.348*	5.334*
CV (%)	51.76		11.66		10.37	34.04

Treatment	Marechal Cândido Rondon			Entre Rios do Oeste
	Yield (kg ha^-1)
	With Mesotrione	Without Mesotrione	Mean	With Mesotrione	Without Mesotrione	Mean
Control	11576.7	11122.3	11345.5	10010.9	9515.7	9778.3
Foliar application of A. brasilense	11169.8	10797.3	10983.6	9671.0	9215.4	9443.2
Seed inoculation of A. brasilense	10504.2	10860.9	10682.5	9880.9	10612.3	10146.6
AX+GB+CK	11678.5	10545.9	11112.2	9261.6	10400.7	9831.1
Mean	11232.3	10818.1		9713.6	9936.0
Calculated F values
Treatment	0.79^ns			1.19^ns
Mesotrione	1.65^ns			0.54^ns
Interaction	0.95^ns			1.94^ns
CV (%)	8.00			8.68