Development and characterization of chitosan-collagen films loaded with honey

Mora-López, David Servín de la; Madera-Santana, Tomás Jesús; López-Cervantes, Jaime; Sánchez-Machado, Dalia Isabel; Ayala-Zavala, Jesús Fernando; Soto-Valdez, Herlinda

doi:10.1590/0104-1428.20230031

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ORIGINAL ARTICLE • Polímeros 33 (3) • 2023 • https://doi.org/10.1590/0104-1428.20230031 copy

Development and characterization of chitosan-collagen films loaded with honey

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Biomaterials developed with biopolymers contribute to the healing process of healthy or diabetic patients. The objective of the present study was to evaluate the effect of honey incorporation (0.3, 0.6, and 1.2 g/100 mL) in chitosan/collagen/glycerol composite films. The Ch/Coll/1.2H films revealed the greatest percentage of elongation (27.10%) and Young´s modulus (65.58 MPa). The barrier properties (WVTR and WVP) exhibited a significant increase when the honey was incorporated into the films. The absorption capacity, solubility, and enzymatic biodegradability were lower in films containing honey. The chemical interactions between the functional groups of the films were verified by FTIR. The morphology studied by SEM confirmed the mixture’s homogeneity. Finally, all formulations exhibited antibacterial properties against Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes, and Salmonella Typhimurium. The aforementioned properties of formulated dressings are suitable for their potential application in chronic wounds.

Keywords:
chitosan; collagen; Apis mellifera; antibacterial films; bioactive films

Formulation	Ch^a a Ch: Chitosan. (g)	Coll^b b Coll: Collagen. (g)	Gly^c c Gly: Glycerol. (g)	H^d d H: Honey. Solute (g/100 mL). (g)
Ch/Coll	1.0	0.5	0	0
Ch/Coll/Gly	1.0	0.5	0.6	0
Ch/Coll/Gly/0.3H	1.0	0.5	0.6	0.3
Ch/Coll/Gly/0.6H	1.0	0.5	0.6	0.6
Ch/Coll/Gly/1.2H	1.0	0.5	0.6	1.2

Formulation	Color parameters				Transparency
Formulation	L*	a*	b*	ΔE	Transparency
Ch/Coll	81.30 ± 0.47^c	-1.79 ± 0.08ª	35.31 ± 1.86^c	ND	1.54 ± 0.03^b
Ch/Coll/Gly	82.01 ± 0.30^c	-1.59 ± 0.07ª	34.23 ± 0.81^bc	4.96 ± 0.90^a	0.96 ± 0.02ª
Ch/Coll/Gly/0.3H	57.94 ± 2.75^b	24.92 ± 2.59^b	54.40 ± 2.13^d	36.17 ± 3.72^b	2.67 ± 0.21^c
Ch/Coll/Gly/0.6H	34.44 ± 1.47^a	32.33 ± 0.70^c	25.34 ± 0.95ª	57.13 ± 2.64^c	3.41 ± 0.21^d
Ch/Coll/Gly/1.2H	35.67 ± 3.05^a	33.27 ± 0.33^c	32.61 ± 2.81^b	54.45 ± 2.20^c	3.74 ± 0.15^d

Formulation	σ_max	ε_b	E
Formulation	[MPa]	[%]	[MPa]
Ch/Coll	71.78 ± 5.5^{^d}	1.66 ± 0.3^{^a}	3882.7 ± 1007.2^{^c}
Ch/Coll/Gly	24.74 ± 6.9^{^b}	8.51 ± 1.9^{^c}	722.5 ± 181.9^{^b}
Ch/Coll/Gly/0.3H	19.84 ± 5.1^{^b}	2.94 ± 1.2^{^ab}	974.9 ± 310.5^{^b}
Ch/Coll/Gly/0.6H	33.06 ± 9.5^{^c}	4.30 ± 1.4^{^b}	1110.3 ± 192.1^{^b}
Ch/Coll/Gly/1.2H	8.61 ± 1.7^{^a}	27.10 ± 2.3^{^d}	65.6 ± 12.9^{^a}

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[1] *madera@ciad.mx; jaime.lopez@itson.mx