Effect of bone morphogenetic protein-7 (BMP-7) on in vitro survival of caprine preantral follicles

Efeito da proteína morfogenética óssea 7 (BMP-7) para a sobrevivência in vitro de folículos pré-antrais caprinos

Valdevane R. Araújo Cleidson M. Gomes da Silva Deborah M. Magalhães Gerlane Modesto da Silva Sônia N. Báo José Roberto Viana Silva José Ricardo de Figueiredo Ana Paula R. Rodrigues About the authors

This study was conducted in order to verify the effect of different concentrations of BMP-7 in the in vitro survival and development of caprine preantral follicles. Fragments of caprine ovarian cortical tissue were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) supplemented with different concentrations of BMP-7 (1, 10, 50 or 100ng/ml). Non-cultured fragments or those cultured for 1 or 7 days were processed for classical histology and transmission electron microscopy (TEM). Parameters such as follicular survival, activation and growth were evaluated. The results showed that, after 1 or 7 days of culture, the percentage of morphologically normal follicles was significantly reduced in all treatments when compared with fresh control, except at 1ng/ml of BMP-7 for 1 day. In addition, the concentration of 10ng/ml of BMP-7 significantly increases follicular diameter from day 1 to 7 of culture. There was no influence of the other concentrations of BMP-7 regarding to the follicular and oocyte diameter. Ultrastructure studies confirmed follicular integrity after 7 days of culture in 1ng/ml BMP-7. In conclusion, small concentrations of BMP-7 can improve the survival and growth of caprine preantral follicles during in vitro culture.

Ovary; preantral follicles; growth factor; bone morphogenetic protein-7; BMP-7; goats

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