Comparison of indirect ELISA and indirect immunofluorescence in detection of antibodies to Neospora caninum in buffaloes (Bubalus bubalis)

Sandro P. Silva Rinaldo A. Mota Eduardo B. Faria Alexandre R. Casseb Livia M.N. Casseb Hilma L.T. Dias About the authors

To compare two serologic tests for detection of antibodies against Neospora caninum in sera from buffaloes, samples were collected from 288 buffaloes of 2 to 10 years of age. To identify the presence of IgG, anti-N. caninum was used for the indirect immunofluorescence assay (IFAT), with title 200 as the cutoff, and for the immunoenzymatic test (indirect ELISA), considering as positive samples with ratio S/P >0.5. There were 153 (53.12%) animals positive for N. caninum by IFAT, whilst 50 (17.36%) animals were reactive in ELISA. The presence of antibodies anti-N. caninum demonstrates that the parasite is circulating among buffaloes raised in Pará State, Brazil. ELISA and IFAT tests could be used to diagnose immunoglobulins against this agent. However there was a weak correlation (Kappa = 0.36) between both tests, considering the IFAT as the gold standard.

Neospora caninum; serology; buffaloes; IFAT; ELISA


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