Does resveratrol reduce cisplatin-induced ovarian damage?

A total of 30 female Wistar-Albino rats constituted the research material. The rats were categorized into three groups: Group 1 was administered one milliliter of 0.9% NaCl solution, Group 2 was administered 7.5 mg/kg cisplatin, and Group 3 was administered 7.5 mg/kg cisplatin and 10 mg/kg resveratrol. Ovaries were extirpated in all groups and subjected to biochemical and histopathological tests. Cisplatin-induced damage to ovarian tissue was graded and scored as the total histopathological findings score. The ovarian function was assessed using immunohistochemical staining for c-kit expression. Rats’ malondialdehyde, catalase, and superoxide dismutase levels were determined.

RESULTS:

The histopathological finding score was significantly higher in Group 2 than in other groups (p<0.05). The superoxide dismutase and catalase levels were significantly higher in Group 3 than in Group 2 (p<0.001 for both cases). The malondialdehyde level was significantly higher in Group 2 than in Group 3 (p<0.001).

CONCLUSION:

The study findings demonstrated that resveratrol reduced ovarian injury and enhanced biochemical parameters following cisplatin-induced ovary damage in experimental models.

KEYWORDS:
Cisplatin; Resveratrol; Rat; Ovary

		Group 1 (Control) (n=10)	Group 2 (Cisplatin) (n=10)	Group 3 (Cisplatin+Resveratrol) (n=10)	p-value
Pigmentation^‡ ‡ n (%),
	None	7 (70.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	<0.001 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.
	Minimal	3 (30.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a a,b Different letters showing significant differences between the groups.	5 (50.0)^a a,b Different letters showing significant differences between the groups.
	Mild	0 (0.0)^a a,b Different letters showing significant differences between the groups.	6 (60.0)^b a,b Different letters showing significant differences between the groups.	4 (40.0)^b a,b Different letters showing significant differences between the groups.
	Moderate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.
Inflammation^‡ ‡ n (%),
	None	7 (70.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	2 (20.0)^b a,b Different letters showing significant differences between the groups.	<0.001 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.
	Minimal	3 (30.0)^a,b a,b Different letters showing significant differences between the groups.	2 (20.0)^b a,b Different letters showing significant differences between the groups.	7 (70.0)^a a,b Different letters showing significant differences between the groups.
	Mild	0 (0.0)^a a,b Different letters showing significant differences between the groups.	4 (40.0)^b a,b Different letters showing significant differences between the groups.	1 (10.0)^a,b a,b Different letters showing significant differences between the groups.
	Moderate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	4 (40.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
Fibrosis^‡ ‡ n (%),
	None	10 (100.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	<0.001 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.
	Minimal	0 (0.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a,b a,b Different letters showing significant differences between the groups.	7 (70.0)^b a,b Different letters showing significant differences between the groups.
	Mild	0 (0.0)^a a,b Different letters showing significant differences between the groups.	5 (50.0)^b a,b Different letters showing significant differences between the groups.	3 (30.0)^a,b a,b Different letters showing significant differences between the groups.
	Moderate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
	Severe	0 (0.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
Congestion^‡ ‡ n (%),
	Minimal	7 (70.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	6 (60.0)^a a,b Different letters showing significant differences between the groups.	0.002 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.
	Mild	3 (30.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a a,b Different letters showing significant differences between the groups.	4 (40.0)^a a,b Different letters showing significant differences between the groups.
	Moderate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	4 (40.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
	Severe	0 (0.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
Hemorrhage^‡ ‡ n (%),
	None	3 (30.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.	<0.001 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.
	Minimal	6 (60.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^b a,b Different letters showing significant differences between the groups.	7 (70.0)^a a,b Different letters showing significant differences between the groups.
	Mild	1 (10.0)^a a,b Different letters showing significant differences between the groups.	2 (20.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a a,b Different letters showing significant differences between the groups.
	Moderate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	6 (60.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
	Severe	0 (0.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^a a,b Different letters showing significant differences between the groups.
	HPF total^§ § median [min–max]. HPF: histopathological findings.	2.0 [1.0–6.0]	12.0 [6.0–17.0]	6.0 [4.0–9.0]	<0.001 ^* * Fisher Freeman Halton test. Bold indicates statistically significant values.

		Group 1 (Control) (n=10)	Group 2 (Cisplatin) (n=10)	Group 3 (Cisplatin+Resveratrol) (n=10)	p-value^* * Fisher Freeman Halton test.
C-kit expression^‡ ‡ n (%).
	Negative	7 (70.0)^a a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	0 (0.0)^b a,b Different letters showing significant differences between the groups.	<0.001
	Weak	3 (30.0)^a a,b Different letters showing significant differences between the groups.	1 (10.0)^a a,b Different letters showing significant differences between the groups.	3 (30.0)^a a,b Different letters showing significant differences between the groups.
	Intermediate	0 (0.0)^a a,b Different letters showing significant differences between the groups.	4 (40.0)^b a,b Different letters showing significant differences between the groups.	5 (50.0)^b a,b Different letters showing significant differences between the groups.
	Strong	0 (0.0)^a a,b Different letters showing significant differences between the groups.	5 (50.0)^b a,b Different letters showing significant differences between the groups.	2 (20.0)^a,b a,b Different letters showing significant differences between the groups.
Biochemical parameters p-value^ Kruskal-Wallis H test, Dwass-Steel-Critchlow-Fligner test for pair-wise comparisons. Bold indicates statistically significant values.
	MDA^§ § median [min–max].	3.2 [3.0–3.5]	8.2 [7.4–8.7]	5.0 [4.7–5.3]	<0.001
	SOD^§ § median [min–max].	30.1 [26.7–33.4]	12.3 [10.8–13.0]	19.4 [17.0–20.8]	<0.001
	CAD^§ § median [min–max].	65.9 [59.2–71.0]	17.1 [15.0–19.1]	37.1 [33.2–41.8]	<0.001

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[1] *Corresponding author: dr.baris_ciplak@hotmail.com