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Micropropagation of Cabralea canjerana

Cabralea canjerana (Vell.) Mart. (Meliaceae) ("canjarana") is a native tree of economic importance in Brazil. The storage of seeds is of short duration and it is therefore necessary to establish a protocol for micropropagation of this species. In this work, multiplication experiments were carried out using nodal segments, excised from in vitro germinated plants. The segments were inoculated in MS or WPM culture medium, supplemented with 6-benzylaminopurine (BAP) and/or 2-isopentenyladenine (2-iP) at 2.5 or 5 µM. Micro-cuttings were taken from new shoots developed from the seeds and used in a rooting experiment using a culture medium with half-strength MS medium (MS/2) supplemented with indolbutyric acid (IBA) (0, 2.5 and 5 µM). After 7 days in this medium, they were transferred to MS/2 medium without auxin under light. During the multiplication phase, the MS culture medium was more suitable for the multiplication of C. canjerana than WPM medium. The nodal segments cultured in the presence of 2.5 µM BAP showed the best result, with a multiplication rate of 1.77 per month on MS medium. The rooting of the microcuttings was 87.5% when they were kept in the presence of 5 µM IBA for 7 days. An acclimatization rate of 90% was achieved after 30 days in the greenhouse. In conclusion, the micropropagation of C. canjerana from nodal segments of plantlets is possible for this species.

Cytokinins; native species; tissue culture


Sociedade de Investigações Florestais Universidade Federal de Viçosa, CEP: 36570-900 - Viçosa - Minas Gerais - Brazil, Tel: (55 31) 3612-3959 - Viçosa - MG - Brazil
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