GENETIC DIVERSITY IN AMBURANA (<i>Amburana cearensis</i>) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

Souza, Ana Valéria Vieira de; Carvalho, Jackson Rafael de Sá; Costa, Evelyn Sophia Silva; Souza, Cristina Soares de; Silva, Herick Fernando de Jesus; Luz, José Magno Queiroz; Maciel, Gabriel Mascarenhas; Siquieroli, Ana Carolina Silva

doi:10.1590/1806-908820210000008

Ana Valéria Vieira de Souza ^**Corresponding author.

Empresa Brasileira de Pesquisa Agropecuária, Petrolina, PE - Brasil. E-mail: <ana.souza@embrapa.br>.

http://orcid.org/0000-0001-5399-0085

Jackson Rafael de Sá Carvalho

Universidade Estadual de Feira de Santana, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Feira de Santana, BA - Brasil. E-mail: <rafael_carvalho@hotmail.com>.

http://orcid.org/0000-0002-2199-1830

Evelyn Sophia Silva Costa

Universidade Estadual de Feira de Santana, Programa de Pós-Graduação em Biotecnologia, Feira de Santana, BA - Brasil. E-mail: <evelyn.sophia@hotmail.com>.

http://orcid.org/0000-0002-7779-2021

Cristina Soares de Souza

Universidade Federal de Uberlândia, Instituto de Ciências Agrarias, Uberlândia, MG - Brasil. E-mail: <cristina.genetica@gmail.com> and <jmagno@ufu.br>.

http://orcid.org/0000-0003-3219-050X

Herick Fernando de Jesus Silva

Universidade Federal de Uberlândia, Programa de Pós-Graduação em Agronomia, Uberlândia, MG - Brasil. E-mail: <herickfernando@gmail.com>.

http://orcid.org/0000-0002-6636-3029

José Magno Queiroz Luz

Universidade Federal de Uberlândia, Instituto de Ciências Agrarias, Uberlândia, MG - Brasil. E-mail: <cristina.genetica@gmail.com> and <jmagno@ufu.br>.

http://orcid.org/0000-0002-5712-7710

Gabriel Mascarenhas Maciel

Universidade Federal de Uberlândia, Instituto de Ciências Agrarias, Monte Carmelo, MG - Brasil. E-mail: <gabrielmaciel@ufu.br>.

http://orcid.org/0000-0002-3004-9134

Ana Carolina Silva Siquieroli

Universidade Federal de Uberlândia, Instituto de Biotecnologia, Uberlândia, MG - Brasil. E-mail: <carol@ufu.br>.

http://orcid.org/0000-0003-4713-1262

*Corresponding author.

6. AUTHOR CONTRIBUTIONS

Conceived and designed the analysis: AVVS, JRSC, ESSC. Material preparation, data collection and analysis: AVVS, JRSC, ESSC, CSS, HFJS. Wrote the paper: AVVS, HFJS, JMQL, GMM, ACSS. Revision and edition of the manuscript: AVVS, JMQL, GMM, ACSS. Research supervision: AVVS, JMQL. All authors read and approved the final manuscript.

Empresa Brasileira de Pesquisa Agropecuária, Petrolina, PE - Brasil. E-mail: <ana.souza@embrapa.br>.

Universidade Estadual de Feira de Santana, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Feira de Santana, BA - Brasil. E-mail: <rafael_carvalho@hotmail.com>.

Universidade Estadual de Feira de Santana, Programa de Pós-Graduação em Biotecnologia, Feira de Santana, BA - Brasil. E-mail: <evelyn.sophia@hotmail.com>.

Universidade Federal de Uberlândia, Instituto de Ciências Agrarias, Uberlândia, MG - Brasil. E-mail: <cristina.genetica@gmail.com> and <jmagno@ufu.br>.

Universidade Federal de Uberlândia, Programa de Pós-Graduação em Agronomia, Uberlândia, MG - Brasil. E-mail: <herickfernando@gmail.com>.

Universidade Federal de Uberlândia, Instituto de Ciências Agrarias, Monte Carmelo, MG - Brasil. E-mail: <gabrielmaciel@ufu.br>.

Universidade Federal de Uberlândia, Instituto de Biotecnologia, Uberlândia, MG - Brasil. E-mail: <carol@ufu.br>.

Table 1 Means testing of DNA content of 68 Amburana cearensis genotypes.

Genotype	Mean	Genotype	Mean	Genotype	Mean
BB116	37.04 a	J44	29.05 f	BB 119	27.84 h
J45	31.95 b	BJ88	28.98 g	LA110	27.80 h
J27	31.33 c	J55	28.89 g	BB117	27.76 h
CA65	31.21 c	RO93	28.87 g	J50	27.70 h
J19	30.99 d	J30	28.73 g	J32	27.65 h
J26	30.87 d	RO95	28.71 g	BJ76	27.63 h
CA135	30.61 d	J51	28.59 g	RO101	27.34 i
CA129	30.56 d	J49	28.53 g	J46	27.30 i
J35	30.51 d	RO100	28.50 g	BB122	27.25 i
RO98	30.43 d	J43	28.47 g	BB120	27.06 i
J57	30.42 d	J40	28.47 g	BB121	27.06 i
J42	30.04 e	RO97	28.40 g	CA66	26.95 i
BJ80	29.92 e	RO109	28.38 g	BJ69	26.87 i
RO94	29.86 e	RO90	28.32 g	BB124	26.71 i
RO99	29.82 e	J41	28.31 g	J20	26.38 j
J53	29.72 e	RO104	28.20 h	BB127	26.30 j
J25	29.60 e	J36	28.16 h	BJ81	26.19 j
J56	29.49 e	LA111	28.16 h	BJ71	26.04 j
BB125	29.32 f	J33	28.13 h	BB126	26.00 j
J54	29.28 f	BB115	27.96 h	J48	25.37 k
RO89	29.19 f	J47	27.92 h	RO107	25.35 k
RO105	29.16 f	J17	27.92 h
J52	29.08 f	J34	27.89 h

Table 2 Division of Amburana cearensis genotypes in different genetic groups based on six hierarchical clustering methods.

Hierarchical	Group	Genotypes
method
Gower	I	BB116
	II	BB115, J46, CA135, BB119, BB127, J33, BB117, J49, BJ71, J30, RO100, J45, BB122, BB120, BB121, CA65, BB123, LA111, BB124, J53, BJ88, RO104, RO90, J54, BJ81, J43, J51, RO101, J35, LA110, J32, RO99, J42, J36, RO95, RO89, RO107, J40, RO94, J27, J48, J50
	III	RO 105, J47, BB125, BJ69, BB126, BJ80, J19
	IV	RO93, RO98, BJ77, J52, J20, J57, J55, J41, CA66, J34, CA129, RO97, J56, J17, J25, RO109, J26, J44
Average Linkage	I	BB116
	II	BJ71, J30, J49, BB119, BB127, BB117, BB115, J46, CA135, J33, RO101, J35, LA110, J32, RO89, RO107, RO95, J40, RO99, J42, J36, J48, J50
	III	RO100, J45, BB122, BB120, BB121, CA65, BB123, LA111
	IV	RO105, J47, BB125, BJ69, BB126, BJ80, J19
	V	CA66, J34, CA129, RO97, J56, J17, J25, RO109, J26, J44
	VI	RO93, RO98, BJ76, J52, J41, J20, J57, J55, RO90, J54, RO94, J27, J43, J51, BJ81, BB124, J53, BJ88, RO104
UPGMA	I	BB116
	II	RO97, J56, CA66, J34, CA129, J17, J25, RO109, J26, J44
	III	RO93, RO98, BJ76, J52, J41, J20, J57, J55, RO90, J54, RO94, J27, J43, J51, BJ81, BB124, J53, BJ88, RO104, RO100, J45, BB122, BB120, BB121, LA111, CA65, BB123, BB115, J46, CA135, J33, BJ71, J30, BB119, BB127, BB117, J49, RO49, RO101, J36, LA110, J32, RO89, RO107, RO95, J40, RO99, J42, J36, J48, J50, RO105, J47, BB125, BJ69, BB126, BJ80, J19
Ward	I	BB116
	II	RO97, J56, CA66, J34, CA129, J17, J25, RO109, J26, J44
	III	RO100, J45, BB122, BB120, BB121, LA111, CA65, BB123, RO105, J47, BB125, BJ69, BB126, BJ80, J19
	IV	BB115, J46, CA135, J33, BJ171, J30, BB119, BB127, BB117, J49, RO101, J35, LA110, J32, RO89, RO107, RO95, J40, RO99, J42, J36, J48, J50
	V	RO93, RO98, BJ76, J52, J41, J20, J57, J55, RO90, J54, RO94, J27, J43, J51, BJ81, BB124, J53, BJ88, RO104
Complete Linkage	I	BB116
	II	RO105, J47, BB125, BJ69, BB126, BJ80, J19, BJ71, J30, BB117, J49, BB15, J46, CA135, BB119, BB127, J33, RO100, J45, BB122, BB120, BB121, LA111, CA65, BB123
	III	RO97, J56, CA66, J34, CA129, J17, J26, RO109, J26, J44
	IV	RO93, RO98, BJ76, J41, J20, J57, J55, J52, J43, J51, BJ81, BB124, J53, BJ88, RO104, RO101, J35, LA110, J32, RO89, RO107, RO95, J40, RO99, J42, J36, J48, J50, RO90, J54, RO94, J27
WPGMA	I	BB116
	II	RO97, J56, CA66, J34, CA129, J17, J25, RO109, J26, J44
	III	RO105, J47, BB125, BJ69, BB126, BJ80, J19
	IV	RO93, RO98, BJ76, J52, J41, J20, J57, J55, BB124, J53, BJ88, RO104, RO90, J54, J43, J51, BJ81, RO94, J27, RO99, J42, J36, J48, J50, RO101, J35, LA110, J32, RO89, RO107, RO95, J40, BJ71, J30, BB115, J46, CA135, J33, BB119, BB127, BB117, J49, RO100, J45

Table 3 Representation of the clustering of 68 Amburana cearensis genotypes by the Tocher optimization method from Euclidean distances based on analysis of molecular markers.

Group	Genotypes
I	RO93, RO98, BJ76, J52, J41, J20, J57, J55, BB124, J53, BJ88, RO104, J51, J43, BJ81, J54, RO90, J27, RO94, J50, J48, RO99, J42, J36, RO95, RO107, RO89, J40, RO101, J35, LA110, J32, BB115, J46, CA135, J33, BB119, BB127, BB117, J49, J30, BJ71, RO100, J45, BB122, BB120, BB121, CA65, LA111, BB123, J56, RO97, J34, CA66, CA129, J19, BB126, BJ80, J25, J17, BJ69, BB125, RO109, J26, J47, RO105, J44
II	BB116
Total	68

[1] *Corresponding author.

Brasil

Brasil

GENETIC DIVERSITY IN AMBURANA (Amburana cearensis) ACCESSIONS: HIERARCHICAL AND OPTIMIZATION METHODS

DIVERSIDADE GENÉTICA ENTRE ACESSOS DE AMBURANA (Amburana cearensis): MÉTODOS HIERÁRQUICOS E DE OTIMIZAÇÃO

ABSTRACT