Dietary Inclusion of Pancreatin Enzyme on the Ileal and Fecal Digestibility of Nutrients in Layer-Type Cockerels

Asare, E; Yang, Z; Zhou, H; Cai, Q; Yang, H; Wang, Z

doi:10.1590/1806-9061-2022-1673

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Brazilian Journal of Poultry Science

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Original Article • Braz. J. Poult. Sci. 25 (01) • 2023 • https://doi.org/10.1590/1806-9061-2022-1673 copy

Dietary Inclusion of Pancreatin Enzyme on the Ileal and Fecal Digestibility of Nutrients in Layer-Type Cockerels

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The current experiment aimed to evaluate the effects of Pancreatin supplementation at different levels on ileal and fecal digestibility in layer-type cockerels. A total of 480-day-old silver brown Hy-Line male chicks were randomly allocated into 5 treatments, 6 replicates (16 birds per pen) arranged in a completely randomized design. Pancreatin enzyme was supplemented on a basal corn-soybean meal-based diet at 0, 250, 500, 750, and 1000 mg/kg and was fed in two growth phases (starter and grower). The results indicated that at the end of the starter stage, except for 1000 mg/kg, dietary Pancreatin supplementation levels increased (p<0.05) the ileal crude protein (CP). Similarly, addition of Pancreatin increased (p< 0.05) apparent ileal amino acids (AA) digestibility (AIAAD) total means of AA (MTAA), means of indispensable AA (MIAA) and dispensable AA (MDAA) with the optimum performance on 250 mg/kg and 500 mg/kg. However, except for histidine and alanine which were negatively affected (p<0.05), and MIAA, MDAA, MTAA which were also positively affected, the addition level at 1000 mg/kg did not affect most of the AIAAD compared to the non-supplemented. Further, Pancreatin supplementation had no effect (p>0.05) on nitrogen digestibility (ND), nitrogen retention (NR), digestible energy (DE), apparent metabolizable energy (AME), dry matter digestibility (DMD), dry matter retention (DMR), and apparent metabolizable energy corrected for nitrogen (AME-n) on fed starter diet. On the other hand, at the end of the grower stage, dietary Pancreatin enzyme supplementation reduced (p<0.05) the ileal CP, MIAA, MDAA, MTAA, AIAAD, AME, AME-n, ND, NR, DE, DMD, and DMR in a dose-dependent manner. The rate of reduction was more marked on Pancreatin addition level 1000 mg/kg. In conclusion, Pancreatin supplementation at 250 mg/kg, 500 mg/kg, and 750 mg/kg improved AIAAD and ileal CP, especially at the young age. The rate of pancreatin enzyme effect was dependent on enzyme supplement level to the ileal CP and individual amino acid.

Keywords:
Amino acid; Corn-SBM-based diet; Digestibility; Energy; Pancreatin

Ingredients (%)	Starter diet¹	Grower diet¹	Analyzed Nutrients	Starter diet¹	Grower diet¹
Corn	66.10	66.25	Dry matter	88.58	89.63
Soybean	29.00	26.10	Gross ME (MJ/kg)	16.00	15.75
Wheat bran	0.20	3.00	Crude protein	18.41	17.94
Methionine	0.20	0.20	Ash	4.71	6.00
Lysine	0.20	0.15	Acid insoluble ash	0.48	0.50
Salt	0.30	0.30	Calcium	1.05	1.04
Limestone	1.30	1.30	Phosphorus	0.74	0.76
CaHPO3	1.70	1.70	Arginine	0.71	0.22
Premix²	1.00	1.00	Histidine	0.18	0.81
Total	100	100	Isoleucine	0.55	0.38
Calculated Nutrients (%)			Leucine	1.10	0.61
Metabolizable Energy (MJ/kg)	11.98	11.85	Lysine	1.01	0.97
Crude protein	18.40	17.48	Methionine	0.46	0.42
Crude fiber	2.78	2.80	Phenylalanine	0.64	0.51
Calcium	1.08	1.07	Threonine	0.45	0.52
Total phosphorus	0.67	0.68	Tryptophan	0.50	1.21
Available phosphorus	0.42	0.43	Valine	0.59	0.07
Lysine	1.13	1.01	Alanine	0.64	0.71
Methionine	0.47	0.45	Asparagine	1.17	1.35
			Glutamine	2.25	2.59
			Glycine	0.50	0.59
			Proline	2.39	0.81
			Serine	0.58	0.67

Item (%)	Control	Pancreatin supplement on control (mg/kg)				SEM	p-Value
Item (%)	Control	250	500	750	1000		Pancreatin	Linear	Quadratic
Crude Protein	80.50^a	83.53^b	82.74^b	82.75^b	80.51^a	0.36	< 0.001	0.003	0.003
Indispensable AA
Arginine	87.03^a	93.13^b	92.01^b	87.66^a	87.60^a	0.59	< 0.001	0.043	0.608
Histidine	85.59^b	91.91^d	90.02^cd	87.22^bc	79.75^a	0.90	< 0.001	0.209	0.087
Isoleucine	82.12^a	88.90^b	87.03^b	87.51^b	79.71^a	0.83	< 0.001	0.022	0.991
Leucine	85.97^ab	90.98^c	88.86^bc	87.50^b	83.65^a	0.62	< 0.001	0.347	0.899
Lysine	82.48^a	91.30^c	89.62^bc	87.61^b	82.96^a	0.79	< 0.001	< 0.001	0.360
Methionine	76.30^a	88.14^b	87.31^b	87.08^b	77.43^a	1.13	< 0.001	< 0.001	0.384
Phenylalanine	86.01^ab	91.31^d	89.57^cd	87.54^bc	84.22^a	0.62	< 0.001	0.192	0.895
Threonine	78.91^a	86.21^b	84.84^b	87.67^b	76.28^a	1.01	< 0.001	0.004	0.974
Valine	81.86^a	88.85^b	86.47^b	87.27^b	79.35^a	0.81	< 0.001	0.028	0.894
MIAA	82.92^a	89.99^b	81.22^a	87.457^b	88.41^b	0.78	< 0.001	0.027	0.519
Dispensable AA
Alanine	84.62^b	88.85^c	87.05^bc	87.33^bc	80.98^a	0.70	< 0.001	0.319	0.537
Asparagine	84.54^a	91.40^c	89.21^bc	88.17^b	82.56^a	0.74	< 0.001	0.039	0.887
Glutamine	88.43^a	93.71^b	91.91^b	87.22^a	87.35^a	0.60	< 0.001	0.586	0.922
Glycine	80.57^a	87.07^b	85.30^b	87.18^b	77.01^a	0.93	< 0.001	0.025	0.752
Proline	88.41^a	93.73^b	91.92^b	87.18^a	87.36^a	0.60	< 0.001	0.583	0.940
Serine	83.84^a	89.84^b	88.21^b	87.72^b	81.95^a	0.71	< 0.001	0.042	0.989
Tyrosine	85.25^ab	90.47^c	88.68^c	87.38^bc	83.08^a	0.65	< 0.001	0.483	0.857
MDAA	85.09^ab	90.72^d	82.90^a	87.45^bc	85.90^cd	0.67	< 0.001	0.202	0.316
MTAA	83.87^a	90.31^b	81.95^a	87.45^b	88.63^b	0.73	< 0.001	0.067	0.427

Item (%)	Control	Pancreatin supplement on control (mg/kg)				SEM	p-Value
Item (%)	Control	250	500	750	1000		Pancreatin	Linear	Quadratic
Crude protein	82.13^c	72.72^ab	74.71^ab	74.05^b	76.87^a	1.24	0.001	< 0.001	0.210
Indispensable AA
Arginine	84.07^c	78.50^a	80.70^b	81.13^b	77.56^a	0.48	< 0.001	< 0.001	0.288
Histidine	85.82^d	78.99^ab	80.13^bc	81.04^c	78.04^a	0.55	< 0.001	< 0.001	0.001
Isoleucine	66.89^e	53.60^b	62.47^d	58.93^c	49.57^a	1.24	< 0.001	< 0.001	0.306
Leucine	80.88^d	72.35^b	76.09^c	75.31^c	69.10^a	0.79	< 0.001	< 0.001	0.963
Lysine	85.30^d	78.01^b	81.31^c	80.70^c	74.89^a	0.68	< 0.001	< 0.001	0.649
Methionine	81.11^d	73.04^b	76.76^c	75.38^c	70.51^a	0.72	< 0.001	< 0.001	0.620
Phenylalanine	83.75^c	75.96^a	80.02^b	78.65^b	75.79^a	0.60	< 0.001	< 0.001	0.060
Threonine	76.32^d	63.60^b	70.96^c	69.10^c	60.56^a	1.09	< 0.001	< 0.001	0.802
Valine	82.85^c	62.21^a	78.04^b	76.27^b	60.78^a	1.68	< 0.001	< 0.001	0.020
MIAA	80.78^d	70.69^b	76.28^c	75.17^c	68.53^a	0.85	< 0.001	< 0.001	0.317
Dispensable AA
Alanine	84.89^d	77.79^b	80.72^c	79.98^c	75.79^a	0.62	< 0.001	< 0.001	0.317
Asparagine	82.58^c	72.80^a	77.58^b	77.56^b	71.54^a	0.78	< 0.001	< 0.001	0.282
Glutamine	89.17^d	83.39^b	85.04^c	85.99^c	81.03^a	0.53	< 0.001	< 0.001	0.556
Glycine	79.11^d	68.63^b	74.29^c	72.44^c	64.30^a	0.99	< 0.001	< 0.001	0.342
Proline	88.40^d	83.03^b	83.83^bc	85.02^c	81.21^a	0.48	< 0.001	< 0.001	0.042
Serine	80.91^d	71.37^b	76.42^c	75.18^c	68.43^a	0.85	< 0.001	< 0.001	0.793
Tyrosine	85.49^d	78.52^b	81.44^c	81.07^c	76.88^a	0.59	< 0.001	< 0.001	0.268
MDAA	84.37^d	76.50^b	79.90^c	79.60^c	74.17^a	0.68	< 0.001	< 0.001	0.606
MTAA	82.35^d	73.24^b	77.86^c	77.10^c	71.00^a	0.77	< 0.001	< 0.001	0.804

Item (%)	Control	Pancreatin supplement on control (mg/kg)				SEM	p-Value
Item (%)	Control	250	500	750	1000		Pancreatin	Linear	Quadratic
Starter diet
DMR (g/g)	0.87	0.87	0.87	0.86	0.88	0.003	0.247	0.287	0.468
NR (g/g)	0.76	0.79	0.77	0.75	0.79	0.006	0.212	0.527	0.987
AME (MJ/kg)	14.26	14.38	14.21	14.25	14.33	0.034	0.593	0.898	0.947
AME-n (MJ/kg)	13.49	13.58	13.43	13.49	13.54	0.030	0.638	0.975	0.937
Grower diet
DMR (g/g)	0.78^c	0.74^b	0.72^b	0.76^ab	0.68^a	0.008	< 0.001	< 0.001	0.744
NR (g/g)	0.58^d	0.49^bc	0.45^ab	0.54^cd	0.37^a	0.018	< 0.001	< 0.001	0.691
AME (MJ/kg)	12.74^d	12.05^bc	11.76^b	12.33^cd	11.16^a	0.120	< 0.001	< 0.001	0.754
AME-n (MJ/kg)	12.18^d	11.56^bc	11.32^b	11.80^cd	10.80^a	0.103	< 0.001	< 0.001	0.770

Item (%)	Control	Pancreatin supplement on control (mg/kg).				SEM	p-Value
Item (%)	Control	250	500	750	1000		Pancreatin	Linear	Quadratic
Starter diet
DMD (g/g)	0.73	0.72	0.70	0.71	0.74	0.009	0.732	0.938	0.215
DE (MJ/kg)	14.02	14.13	13.99	13.92	14.20	0.04	0.221	0.583	0.399
ND (g/g)	0.67	0.66	0.61	0.69	0.67	0.01	0.381	0.981	0.263
Grower diet
DMD (g/g)	0.74^c	0.59^a	0.69^b	0.66^b	0.56^a	0.013	< 0.001	< 0.001	0.530
DE (MJ/kg)	12.51^c	11.92^b	11.75^b	12.10^bc	10.91^a	0.121	< 0.001	< 0.001	0.202
ND (g/g)	0.77^d	0.64^b	0.66^b	0.70^c	0.60^a	0.009	< 0.001	< 0.001	0.019

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[1] Corresponding author e-mail address
Haiming Yang College of Animal Science and Technology, Yangzhou University, Wenhui East Road 78#, Yangzhou City, Jiangsu Province 225009, People’s Republic of China. Phone: +86-514-87979045 Email:yhmdlp@qq.com