ABSTRACT
Ten-week-old Langde geese in similar body weight were randomly selected, four for overfeeding and four for routinly feeding. The abundance of liver fatty acid-binding protein (L-FABP), thyroid hormone-responsive (THRSP or Spot 14), obese (OB), and apolipoprotein A1 (Apo A1) genes in goose were detected by quantitative RT-PCR. L-FABP was higher expressed in liver and intestine than other tissues, but no expression was detected in the pancreas or brain. The other three genes were widely expressed in different tissues, OB was higher expressed in pancreas and abdominal fat, Spot 14 and Apo A1 was higher expressed in sebum and abdominal fat. Spot 14 and Apo A1 genes were up-regulated in overfed goose livers compared with that in the control. Thus, Spot 14 and Apo A1 genes may play important roles in lipid metabolism in goose fat liver.
Keywords:
Langde goose; lipids related genes; gene expression; fatty liver
Expression levels of these genes in the different tissues were determined by quantitative RT-PCR and normalized to that of GAPDH. No L-FABP gene was detected in the pancreas or brain. Each column in each diagram represents the mean ± SEM of four individuals. This condition applies to the following diagram. Different letters indicate significant difference at p<0.05.
Expression levels of Spot 14 and Apo A1 genes in overfed (OF) geese were significantly higher than those in the control group (p<0.05).