Macroanatomy of Cecum in German Mast Geese (Anser Anser) and Investigation with Scanning Electron Microscope (Sem)

ABSTRACT

Our study aimed to compare the cecum of German mast geese with other poultry species through macroanatomy and scanning electron microscope (SEM). Adult German geese were used as research material, which were obtained from its breeders and died of various causes. In the study, after determining the macroanatomical features of the German mast goose cecum, it was examined with a scanning electron microscope (JEOL JSM-5600LV) and the general structures were photographed. The craniodorsal of the rectum of German mast geese was shaped with two ceca, giving right and left arms to the cranial symmetrical to each other near the end of the ileum. The macroanatomy of the apex, corpus, and basis cecum parts was examined in the cecum divided into two. In the study, it was determined that villus extensions were sparse in the right and left apex ceci parts in the cecum SEM images of the German mast geese, while the number of the corpus and basis ceci parts was higher.

As a result, the macroanatomy and scanning electron microscopic findings of German mast geese cecum were examined in detail and its differences from other poultry species were revealed.

Keywords:
Anatomy; Digestive system; Embden goose; SEM

INTRODUCTION

Goose is a large bird species that forms the genus Anser from the Anatidae family of the goose (Anseriformes) order (Tan & Özdemir, 2020; Tan, 2015). Due to different needs and consumption purposes, such as goose farming meat, liver, and feathers, the importance of this bird has increased among poultry in different regions of the world. German mast geese are a breed belonging to Germany, also known as Embden (Emden) goose (Evcil kaz, 2021). It is a goose breed with white feathers, upright posture, orange-colored beak and legs (Tan, 2015; Al-Sharoot, 2016). Since the Embden goose is very valuable in terms of meat production in recent years, it is cultivated in many parts of the world (Tan, 2015; Evcil kaz, 2021). The digestive system anatomy of domestic birds is quite different from other mammals. Moreover, it has been reported that there are many structural differences among poultry according to their species and feeding habits (Getty, 1975; Elsheikh et al., 2014). Many studies on macroanatomical and SEM studies of different poultry species cecum can be encountered in the literature (Ilgun et al., 2017; Li et al., 2018). The cecum has the characteristics of digesting cellulose and being the organ from which immune system cells are produced in the poultry digestive system (Karadağ & Nur, 2002; Potter et al., 2006). Although the shape and location of the cecum differ anatomically according to the poultry species, it has been reported that cecum is not present in poultry families such as apodiformes, columbiformes, cuculiformes, piciformes, and psittaciformes (Doğuer & Erençin, 1964; Nomenclature, 1993; Ilgun et al., 2017). Cecum in poultry lies between the ileum and the colon, curved to the left and right. Its form is that of a closed tube with its length and dark green appearance. (Abas, 2013). The cecum consists of three parts: apex, corpus, and basis (McLelland, 1990; Karadağ & Nur, 2002; Haligür, 2008). Apex ceci tilts to the right and left depending on the species.

The head is in the form of a blind pouch. (Tasbas, 1978; Mclelland, 1989; Karadağ & Nur, 2002). While the corpus ceci is a short median part, it has a weak and thin lumen (Nickel et al., 1977; Karadağ and Nur, 2002). The basis ceci part forms the long part of the cecum, with its thick wall and narrow lumen appearance (Chen et al., 2002; Abas et al., 2013; Karadağ & Nur, 20021). The cecum is connected to ileum by the ligamentum ileocecale (Nickel et al., 1977; Haligur, 2008). There are various studies in the literature (Mclelland, 1989; Chen et al., 2002; Ilgun et al., 2018) with SEM findings of the cecum.

The main purpose of the study is to make a scientific contribution to activities such as research, crossbreeding, and breeding for geese of various species in light of findings to be obtained from the macroanatomical and SEM images of the German mast goose cecum.

MATERIALS AND METHODS

The ceca of geese with an unknown cause of death were examined. Morphological examination of the cecum was performed after dissection of the research material. Cecum tissue samples taken for scanning electron microscope (SEM) images were modified by the methods of Chen, Hsu, & Hsu, (Chen et al., 2002) and Erdoğan & Alan (Erdogan & Alan, 2012). Parts of apex, corpus, and basis ceca were kept in 10% formaldehyde for 24 hours. After washing with 0.1 M PBS (Phosphate buffer solution) 2 times for 10 minutes, they were kept in 2.5% glutaraldehyde for 6 hours and washed 5 times in 0.1 M buffered buffer solution. They were left to stand for 10 minutes in 25%, 50%, 75% and 100% Ethyl Alcohol series. After drying and coating process in the incubator, their images were taken with a SEM (JEOL JSM 5600 LV) located at the Eskişehir Osmangazi University Central Research Laboratory Application and Research Center. Nomina Anatomica Veterinaria (ICVGAN, 2017) and Nomina Anatomica Avium (Baumel et al., 1993) were used for writing terminological expressions.

ETHICAL APPROVAL

This study was carried out in accordance with the ethical rules set forth by decision numbered 2020/14, which was taken by Fırat University Animal Experiments Local Ethics Committee. Geese were obtained from German Mast goose breeders in Elazig province.

RESULTS

Macroanatomical result

The cecum of the German mast goose was between the craniodorsal of the gray rectum and the end of the ileum, and was located in two curved extensions on the right and left. These two folds were closed at the ends and there were two ceca towards the cranial. It is anatomically located in the ventral of Cavum abdominis. It was determined that it is connected to the ileum through the ligamentum ileocecale. Apex, corpus, basis ceci bodies were present in both ceca. Right and left apex ceci were determined to be long and flat. The cecum formed a common path with the last part of the ileum and opened to the rectum with a short colon course after the ostium cecum opening. (Figure 1).

Figure 1
Macroanatomical view of Cecum.

Scanning electron microscope (SEM) result

SEM images of the apex, corpus, and basis ceci parts of the German mast excavation right and left cecum were examined. The villi in the cecum formed finger-shaped extensions towards the lumen. While these extensions were sparse in the right and left apex ceci parts, it was determined that there were more in the corpus and basis ceci parts. The serous and muscular layers were evident in all parts. Epithelium was densely detected on the corpus ceci.

Figure 2
SEM views of Cecum. A. Apex ceci, B. Basis ceci, C. Corpus ceci. v.villi, e. Epithelial tissue, m. Tunica muscularis, p. Serosa layer.

DISCUSSION AND CONCLUSION

It has been reported that the cecum in poultry is dark green, and its right and left ends are closed as a tube, between the ileum and the colon (Abas et al., 2013). The research material was also found in the cecum gray, cavum abdominis in German mast geese and showed other similar findings.

Ilgün et al. (Ilgun et al., 2018) reports that the cecum of the guinea fowl has a more pointed left apex ceci, while the right apex ceci is longer and flattened. No difference was observed in the right and left apex ceci in German mast geese.

Doğuer & Erençin, (Doguer & erencin, 1964), Nickel, et al. (Nickel et al., 1977) and Karadağ & Nur, (Karadağ & Nur, 2002); reported that the cecum is attached to the ileum by the ligamentum ileocecale in poultry. Studies show that there is a similar connection in the German mast goose, they reported that the cecum consisted of three main parts: apex, corpus, and basis. Cecum, apex, corpus reported that it consists of three parts on a basis (Mclelland, 1989; Karadağ & Nur, 2002; Haligur, 2008). In German mast goose, the cecum similarly consisted of three parts.

Nickel et al. (Nickel et al., 1977) and Mclelland, (Mclelland, 1989) reported that the basis ceciin parrots, swallows, and pigeons followed a short course to the caudal and opened to the rectum. Barnes & Thomas (26) reported that in owls, the cecum expands towards the caudal rectum.

Gosomji et al. (Gosomji et al., 2015) reported that the 12-13-day-old guinea fowl cecum was opened to the rectum without forming a colon, and the colorectum was opened to the cloaca by reaching out to the ileum in the distal region in a small way. In the German mast goose, both ceca formed a common path attached to the last part of the ileum. After the ostium ceci opening, it opens to the rectum with a short colon course.

Chen et al. (Chen et al., 2002), when analyzing SEM images of the cecum in White Roman geese, reported that the villi are finger-shaped extensions in the filamentous shape and structures. Moreover, it was determined that the length of the villi in the apex ceci part decreased. It was observed that the surface epithelium in the corpus ceci had a circular structure.

Ilgün et al. (Ilgun et al., 2018) reported that in SEM images of guinea fowl cecum, the extensions of the villi in the lumen surround the lumen in a tight and circular manner. The SEM findings of the German Mast goose cecum were also similar to those of White Roman geese and Bec chicken cecum.

REFERENCES

• Abas AN, Iraj P, Hassan G, Sohrabihaghdoust I. Morphology of large intestine in ostrich chickens. Annals of Biological Research 2013;4(2):297-301.
• Al-Sharoot HA. Anatomical, histological and histochemical architecture of pancreases in early hatched goose (Anser anser). Kufa Journal For Veterinary Medical Sciences 2016;7(1):147-56.
• Barnes GG, Thomas VG. Digestive organ morphology, diet and guild structure of North American Anatidae. Canadian Journal of Zoology 1987;65:1812-7.
• Baumel JJ, King AS, Breazile JE, Evans HE, Vanden Berge JC. Nomina anatomica avium. Cambridge: Academic Press;1993.
• Chen HY, Hsu KH, Hsu JC. Studies on the Fine Structure of Caeca Geese. Asian-Australian journal of Animal sciences 2002;15:1018-21.
• Doguer S, Erençin, Z. Comparative anatomy of domestic birds. Ankara: Ankara Veterinary Medicine Publishing; 1964.
• Erdogan S, Alan A. Gross anatomical and scanning electron microscopic studies of the oropharyngeal cavity in the eurpean magpie (Pica pica) and the common raven (Corvus corax). Microscopy Research Techique 2012;75:379-87.
• Erdogan S, Iwasaki S. Function-related morphological characteristic and specialized structures of the avian tongue. Annals of Anatomy 2014;196:75-87.
• Evcil kaz. Partners; 2021. Available from: https://tr.wikipedia.org/wiki/Evcil_kaz
  » https://tr.wikipedia.org/wiki/Evcil_kaz
• Elsheikh EH, Al-Zahaby Sh A. Light and scanning electron microscopial studies of the tongue in the hooded crow (Aves:Corvus corone cornix). The Journal of Basic and Applied Zoology 2014;67:83-90.
• Getty R. Sisson and Grossman's the anatomy of the domestic animals. Lansing: Saunders; 1975.
• Gosomji IJ, Salami SO, Nzalak JO, Kawu MU, Omirinde JO, Wanmi ND. Morphological development of the gastrointestinal tract of helmeted guinea fowl (numida meleagris) at pre-hatch and post-hatch. Journal of Veterinary Anatomy 2015;8:17-27.
• Haligür A. Cecal anatomy in long-legged buzzard (Buteo rufinus). Journal of Erciyes University Faculty of Veterinary Medicine 2008;5(1):11-6.
• Hamedi S, Shomali T, Akbarzadeh A. Prepubertal and pubertal caecal wall histology in Japanese Quails (Coturnix coturnix japonica). Bulgarian Journal Veterinary Medicine 2013;16(2):96-101.
• ICVGAN - International Committe on Veterinary Gross Anatomical Nomenclature. Nomina anatomica veterinaria. 6th ed. Gent: Knoxville; 2017. p.15-25.
• Ilgün R, Gür FM, Bölükbas F, Yavuz O. Macroanatomical and histological study of caecum of the guinea fowl (Numida meleagris) using light and scanning electron microscopy. Indian Journal Animal Research 2018;52(6):858-63.
• Kadhim AB, Dali EI, Sharoot HA. Histomorphological study of duodenum of goose (Anser anser). Al-Qadisiyah Journal of Veterinary Medicine Sciences 2018;17(2):43-8.
• Karadag H, Nur IH. Systema digestorium. In: Dursum N, editor. Anatomy of domestic birds. Ankara: Medisan publication; 2002. p.55-6.
• Li Y, Yang H, Xu L, Wang Z, Zhao Y, Chen X. Effects of dietary fiber levels on cecal microbiota composition in geese. Asian-Australasian Journal Animal Sciences 2018;31(8):1285.
• McLelland JA. Color atlas of avian anatomy. London: Mosby International;1989.
• Nickel R, Schummer A, Seiferle E. Anatomy of the domestic birds. Berlin: Parey; 1977.
• Potter MA, Lentle RG, Minson CJ, Birtles MJ, Thomas D, Hendriks WH. Gastrointestinal tract of the brown kiwi (Apteryx mantelli). Journal of Zoology 2006;270:429-36.
• Tan I, Özdemir D. The comparative macro-anatomical studies on neurocranium of domestic duck (Anas domesticus) and domestic goose (Anser domesticus). Atatürk Üniversitesi Veteriner Bilim Dergisi 2020;15(3):237-43.
• Tan I. The comparative macro-anatomical studies on neurocranium of domestic duck and domestic goose [master thesis]. Erzurum (TR): Atatürk University; 2015.
• Tasbas M. Comparative macro-anatomic and subgross investigations on the digestive system of the hen-cock (Gallus domesticus) and the Turkey (Meleagris gallopavo). Ankara Universitesi Veteriner Fakültesi Dergisi 1978;25(3):500-16.

• FINANCIAL SUPPORT

  This research received no grant from any funding agency/sector.

Publication Dates

• Publication in this collection
  06 Jan 2023
• Date of issue
  2023

History

• Received
  26 Oct 2021
• Accepted
  25 July 2022