The objective of this work was to develop a method for coumarin(1,2-benzopyran) dosage in fluid extract and tinture of "guaco" (Mikania glomerata Sprengel). First derivative spectrophotometry was developed and proved to be accurate, exact, reprodutive and of easy execution.
Mikania glomerata; Guaco; Coumarin; First Derivative spectrophotometry; Fluid extract