Acclimatization is a phase of micropropagation associated with frequent losses in plant survival. The in vitro culture conditions may determine in some species, the formation of plants with altered morphology, anatomy and physiology that affect their acclimatization. The objective of the present work was to evaluate the rate of plant survival during acclimatization of the Prunus rootstocks Capdeboscq and GF677, and the selections VP411 and VP417. Shoots of 2-3 cm long were inoculated in rooting Lepoivre medium supplemented with IBA (0.1, 0.5, 1.0 and 2.0 mg.L-1). After 15 days, the plants were transferred to styrofoam trays with cells containing commercial mixed soil Plantmax®. The trays were placed in plastic boxes closed with a transparent glass plate and maintained in acclimatization room in 27±1ºC temperature, light period of 16 hours and 60mmol.m-2.s-1 of light irradiation, and then transferred to intermittent nebulization chamber. The plant survival rate was significantly affected for the IBA treatments, genotype and genotype x IBA concentration interaction. The best survival rates obtained were Capdeboscq (92%) with 1.0 mg.L-1 IBA, VP417 (80%) with 0.5 mg.L-1 IBA, VP411 (84%) and GF677 (64%), respectively with 0.1 mg.L-1 IBA. The callus formation on the basal region of explants negatively affected the plant survival.
Peach tree; in vitro propagation; IBA; plant survival
