The rhizogenesis is considered a critical stage, since it determines the plant survival during the acclimatization. Among the determinative factors in the induction and the formation of in vitro roots, is distinguished the genotype and the auxin. The objective of the work was to evaluate the effect of different concentrations of acid 4-indol-3-butírico (IBA) in the in vitro rooting in genotype of Genipa americana L; as well as the process of acclimatization of the plant using different substrate. For the in vitro rooting stage, shoots of 2.0 cm long, proceeding from the culture medium with 6-benzilaminopurina (BAP) in concentrations of 2,22; 8,87; 17,74 and 26,76 µM plus the witness with absence of the BAP, were inoculated in medium MS supplemented with IBA (4.9; 9.8 e 14.7 µM) plus the witness with IBA absence. To the acclimatization process it was used as supporting material washed sand, Plantmax® HT and Ecoterra®. The variables analyzed were: percentage of rooting, number of adventitious roots, and length of the largest root, percentage of survival and height of the plant in the acclimatization phase. The results showed that IBA at 9.8 mM induced greater rooting percentage. The substrate Ecoterra® influenced significantly in the quality of root system and quality of the aerial part.
rhizogenesis; auxin; tissues culture
