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EXPLANTS GROWTH AND OXIDATION OF BANANA CV. PRATA (Musa AAB) IN VITRO: I. IRON, COPPER, AND ZINC SALT CONCENTRATIONS

This research work was carried to verify the effects of salt concentrations with iron, copper and zinc of the MS (MURASHIGE & SKOOG, 1962) medium on explants oxidation control for banana plants, cv. Prata (Musa AAB). Three salt concentrations (100, 50, 0 muM) of FeEDTA and two concentrations (100, 0 muM) of (CuSO4). 5H2O and two concentration (30 e 0 muM) of (ZnSO4). 7H2O were utilized. Completely randomized in a 3x2x2 factorial scheme with fifteen repetitions were utilized. Shoot tip apices were inoculated in MS medium. Explants fresh matter weight, height and oxidation grade were evaluated 28 days after inoculation. It was observed that iron, copper and zinc are essential to explants growth. Iron concentration reduction caused decrease in oxidation grade, the reduction or elimination of iron, copper and zinc in MS medium, isolated or in combination forms was not sufficient to eliminate the oxidation grade.

tissue culture; Musa AAB; phenolic oxidation; iron; copper and zinc


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