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Characterization of three mume genotypes (Prunus mume Sieb. et Zucc.) by RAPD markers

A research project with the objective do develop mume clones (Prunus mume Sieb. et Zucc.), to be used as rootstocks for peach tree [Prunus persica (L.) Batsch] is been carried out at the Faculdade de Ciências Agrárias e Veterinárias (FCAV/UNESP), Jaboticabal Campus, São Paulo State, Brazil. These project showed promising perspectives of success, with three clones that were selected according to their characteristics for peach rootstock. But the distinction of the three clones among them, based only in morphologic characteristics, has presented limitations. The objective of the present research was to identify RAPD markers able to characterize and differentiate the 05 and 15 Clones and Rigitano mume cultivar, using Aurora-1 and Okinawa peach tree as outgroup. Among the 220 tested primers, 42 were selected and used to amplify the five genotypes. RAPD markers allowed the distinction among 05 Clone, 15 Clone and Rigitano, selected in FCAV/UNESP, showing presence of genetic diversity among them. These markers also showed that among the three mume genotypes studied, the genetic similarity is bigger between 05 Clone and 15 Clone.

stone fruits; rootstock; molecular markers


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E-mail: rbf@fcav.unesp.br