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MICROPROPAGATION OF BANANA TERRA USING TEMPORARY IMMERSION BIOREACTORS

Banana seedlings has been micropropagated and sold to the producers to supply a rather competitive fruit crop market. This high quality propagule has usually a higher price in the market than field propagated seedlings. Several factors contribute to its final costs: need of specialized labour, need of well equipped laboratory and acclimatization structure, low multiplication rate of some varieties etc. The work presented here reports the development of a new way to micropropagate banana var. Terra, a known slow seedling producing variety, by using a temporary immersion bioreactor. The aim of this work was to increase the multiplication rate of this variety of banana and to reduce the costs of production of the micropropagated seedlings. The results showed that the immersion cycle of 4 hours with medium culture renewed at 30 days was essential to a higher biomass and explants growth. The composition of the medium culture positively influenced the development of the banana explants cultured in the bioreactors. Cultured explants in MS medium + 3 mg/L of BAP changed to a basic MS medium after 30 days presented the higher biomass and multiplication rate of all treatments. Traditional cultivation using semi-solid media was compared to a temporary immersion system and the results showed that the temporary immersion system presented 2.86 times more biomass production and 2,20 times more viable shoots than the traditional semi-solid system.

micropropagation; bioreactor; temporary immersion; banana


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