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Development of in vitro diploid banana plantlets obtained from embryo culture

The objective of this work was to evaluate the development of in vitro plantlets of progenies of eight diploid banana genotypes that were obtained from embryo culture. The genotypes were the wild species Calcutta and Malaccensis (Musa acuminata-AA), Butuhan and França (M. balbisiana-BB) and the hybrids 0304-02, 1304-06, 4252-04 and 9379-09 (M. acuminata-AA). The embryos were aseptically, being introduced in MS medium with 30 g L-1 agar, initially in Petri dishes (40 days) and afterwards in test tubes (45 days). It was observed a pronounced effect of the genotype on the in vitro development of the embryos. The progenies of the wild genotypes of the BB group, followed by that of AA hybrids showed higher development for the studied variables. The protocol was appropriated for the embryo culture of progenies of the eight genotypes. However, in order to have a non accentuated rooting, the period of in vitro development should be reduced to 30 days.

Musa spp.; hybrids; seeds; in vitro germination


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