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Light type in the in vitro multiplication of raspberry plants (Rubus idaeus L.) 'Batum'

The aim of this work was to determine a possible light type more active than the white light, in way to increase the efficiency of the in vitro multiplication of raspberry 'Batum', in relation to the shoots and leaves number, and the multiplication rate. For that, the treatments consisted of five different light types (white - control, red, yellow, blue and green), under which the explants grew, supplied through the modification of the luminous spectrum of the white-cold fluorescent lamps, using colored filters of acetate cellulose, Lee Filters type (Walworth Ind. Estate, Andover, England). The culture medium was constituted of MS salts and vitamins, added of myo-inositol (100mgL-1), sucrose (30gL-1), agar (6gL-1) and BAP (4.44µM). The efficiency of the in vitro multiplication of raspberry 'Batum', in relation to the analyzed variables, increased with the use of green light. The red light also increased the shoots mean number, however, its morphology was modified, with a smaller leaves expansion, thin shoots and prolonged internodes.

tissue culture; luminous spectrum; raspberry; small fruits


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