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In vitro establishment and multiplication of Prunus rootstocks

The success of the modern fruit production depend on the genetic and sanitary quality of plants. For the peach tree culture, micropropagation techniques allow a mass clonal production with high genetic quality plants. The present study aimed to evaluate the explant survival rate during in vitro establishment period, as well as to evaluate the in vitro multiplication potential of Prunus rootstocks. Apexes and lateral buds of the rootstocks Capdeboscq and GF677 and of the selection VP411 were introduced in vitro in the Lepoivre's medium supplemented with BAP (0.5mg.L-1). The mean values of explant survival for the rootstocks were 62.9% and 58.8%, for apexes and lateral buds, respectively. Cultures of apexes and lateral buds showed 14.8% and 29.8% of contamination, respectively. The genotype significantly affected the in vitro multiplication rates. The rootstocks Capdeboscq and GF677, and the selection VP411 showed multiplication rates of 14.7, 10.5 and 16.0 shoots/explant, respectively. As mean values for shoot height of the rootstocks Capdeboscq and GF677 and of the selection VP411, it was observed 9.3, 8.9 and 7.8 mm, respectively. The rootstock Capdeboscq showed superior values as regarded to the number of shoots >20mm as compared to rootstock GF677 and selection VP411 with values of 2.0, 1.3 and 1.0 of shoots, respectively.

Peach tree; rootstock; BAP; micropropagation; plant stock


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