ABSTRACT
In pineapple postharvest, black rot disease is considered as the main problem of fruits trade. The disease is caused by the fungus Chalara paradoxa (De Seynes) Sacc.. Despite the importance of this pathogen to pineapple, there is little information related to it, based on physiological characteristics in vitro. The objective of the study was to evaluate the mycelial growth, sporulation and germination of C. paradoxain different growing conditions. Mycelial discs were removed from pure cultures and placed for growing on petri plates containing the medias: potato-dextrose-agar (PDA), oat-agar (OA), coconut milk-agar (CMA), and pineapple juice agar (PJA).Incubation of the plates was done at three different light regimes and three different temperatures. For mycelial growth, sporulation and germination the best conditions of in vitro cultivation of C. paradoxa were OA and PDA medias in alternating light at 25 ° C, the PDA and PJA medias in continuous darkness at the same temperature.
Index terms
Ananas comosus L; mycelial growth; sporulation; germination
