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Using ISSR for fingerprinting accessions of biribazeiro

The biribazeiro is a fruit plant native from Amazonian and Atlantic forests, in Brazil. Their fruits have great popular acceptance for fresh consumption. The objective of this study was to measure the genetic divergence of biriba genotypes (Rollinia mucosa [Jacq.]Baill) using ISSR molecular markers. Sixteen genotypes of biriba were screened with 20 ISSR primers, which produced a total of 118 bands, with 96 polymorphic and 22 monomorphic fragments. The genetic dissimilarity values ranged from 0.0909 to 0.5147, based on the complement of the Jaccard index. The UPGMA (Unweighted Pair Group Average Method) grouped the accessions into six groups. The genotypes 1 and 5 were most dissimilar and 11 and 12 the most similar. The ISSR markers used in this study demonstrated the efficiency of molecular polymorphisms detection, revealing high genetic variability among the 16 accessions. So, it can be inferred that there is a considerable genetic variation among accessions of the biribazeiro, showing the importance of molecular markers in the analysis of variability of species poorly studied, as Rollinia mucosa [Jacq.]Baill.

Rollinia mucosa; Annonaceae; genetic variability; biribá


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