From Humirianthera ampla, Icacinaceae, were isolated a phthalate, lupeol, β-sitosterol, glycosyl- sitosterol, one known annonalide diterpene and two new diterpenes named Humirianthol and Acrenol. Humirianthol and Acrenol were determined by 1D and 2D NMR spectroscopic techniques to be 3 β, 20:14 β, 16-diepoxy-3 α, 15 α-dihydroxy-7-pimaren-19, 6 β-olide and 3 β, 20-epoxi-3 α, 15, 16-trihydroxy-7-pimaren-19, 6 β-olide, respectively. Acrenol has antimicrobial activity.