Resumo
Artemisinin is a sesquiterpene lactone used in treatment of chloroquine-resistant malaria. This paper presents high-performance liquid chromatographic assay for artemisinin in leaves of A. annua using differential refractometer detector and a single step of clean-up in a silica cartridge. The average of recoveries were 95% and the limit of quantification was 0,21% p/p using 200 mg of the leaves. This method was found to be simple, robust and relatively rapid.
Dosagem de artemisinina em Artemisia annua L. por cromatografia líquida de alta eficiência com detecção por índice de refração
Vera Lúcia Garcia RehderI,* * rehder@cpqba.unicamp.br ; Marili Villa Nova RodriguesI; Adilson SartorattoI; Mary Ann FoglioII
IDivisão de Química Orgânica e Farmacêutica do CPQBA, UNICAMP, Paulínia, SP
IIDivisão de Fitoquímica do CPQBA, UNICAMP, Paulínia, SP
ABSTRACT
Artemisinin is a sesquiterpene lactone used in treatment of chloroquine-resistant malaria. This paper presents high-performance liquid chromatographic assay for artemisinin in leaves of A. annua using differential refractometer detector and a single step of clean-up in a silica cartridge. The average of recoveries were 95% and the limit of quantification was 0,21% p/p using 200 mg of the leaves. This method was found to be simple, robust and relatively rapid.
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Datas de Publicação
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Publicação nesta coleção
05 Out 2009 -
Data do Fascículo
2002
