Open-access The antiproliferative action of morin flavonoid and olive leaf extract (Olea europaea L.) against the cell line H460

ABSTRACT

This study investigates possible apoptosis induction mechanism by the flavonoid morin and the olive leaf extract (Olea europaea L.) in non-small lung cancer cells (H460). The treatment with morin and olive leaves extract resulted in growth inhibition and induction of apoptosis in H460 cells lines measured by the MTT assay methods and confirmed by fluorescence microscopy, flow cytometry analysis. The data indicated that themurin and the extract of olive decreased the cell viability percentage rates of 7.22 ± 1,54% and 62.37 ± 2,85% in the concentrations of 800 µM and µg/mL, respectively. The highest percentage rates of cell death by apoptosis were 100% for themorin in a concentration of 800 µM and 70.49 ± 5.91% for the olive extract in a concentration of 800 µg/mL. These findings were associated with altered mitochondrial membrane potential, whose value is 54.91% for the murin concentration of 400 µM and 42.2% for the olive extract in a concentration of 800 mg / mL, suggesting involvement of the intrinsic pathway of cell death by apoptosis. Therefore, the morin and the olive extract affect the cell viability of H460 cell lines inducing cell death by apoptosis.

Keywords H460 line cell; morin; olive leaf extract; antitumor activity

location_on
Sociedade Brasileira de Plantas Medicinais Sociedade Brasileira de Plantas Medicinais, Revista Brasileira de Plantas Medicinais, Universidade Estadual de Maringá, Departamento de Farmácia, Bloco T22, Avenida Colombo, 5790, 87020-900 - Maringá - PR, Tel: +55-44-3011-4627 - Botucatu - SP - Brazil
E-mail: revista@sbpmed.org.br
rss_feed Stay informed of issues for this journal through your RSS reader
Acessibilidade / Reportar erro