Viability of Tritrichomonas foetus after freezing with diferents cryoprotectors

Nelson Éder Martins Francisco Carlos F. Lobato Felipe M. Salvarani Rodrigo Otávio S. Silva Geraldo Márcio da Costa Catarina G.R.D. Lima Prhiscylla S. Pires Ronnie A. de Assis About the authors

The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196ºC. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively.

Trichomoniasis; glycerol; dimethilsulphosyde (DMSO)


Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br
Accessibility / Report Error