<i>Neospora caninum:</i> evaluation of vertical transmission in slaughtered dairy cows (<i>Bos taurus</i>)

Macedo, César Augusto Barbosa de; Macedo, Madlaine Frigo Silveira Barbosa de; Cardim, Sergio Tosi; Paiva, Milaine Cristiane Dantas Custódio; Taroda, Alessandra; Barros, Luiz Daniel; Cunha, Ivo Alexandre Leme da; Zulpo, Dauton Luiz; Garcia, João Luis

doi:10.1590/S1984-29612013000100004

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Full Article • Rev. Bras. Parasitol. Vet. 22 (1) • Jan-Mar 2013 • https://doi.org/10.1590/S1984-29612013000100004 linkcopy

Neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (Bos taurus)

Neospora caninum: avaliação da transmissão vertical em vacas leiteiras (Bos taurus)

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstracts

Neospora caninum is a worldwide parasite recognized as one of the main parasites responsible for abortion in cattle. The objective of this study was to evaluate vertical transmission of N. caninum in dairy cows (Bos taurus) that were slaughtered at an abattoir in the state of Santa Catarina, southern Brazil. Blood samples (with and without EDTA) from 60 pregnant dairy cows and blood and tissue samples (brain, lung, heart and liver) from their fetuses were collected and used for PCR and serological evaluation. Blood samples from 60 non-pregnant cows were collected and used to detect antibodies. Anti-N. caninum antibodies were detected by indirect ELISA. Antibodies against N. caninum were observed in 41.6% (25∕60) of the pregnant cows and in 43.3% (26∕60) of the non-pregnant cows. Antibodies against the parasite were detected in sera from three fetuses (5.5%). PCR analysis revealed that 3.3% (2∕60) of the cows and 6.6% (4∕60) of the fetuses evaluated were positive for specific N. caninum primers. These positive fetuses were between 4-6 months of age. Therefore, considering PCR and serological tests to be indicative of vertical transmission in fetuses, 11.6% (7∕60) of the fetuses were infected by N. caninum during gestation.

Neospora caninum ; dairy cattle; vertical transmission; Bos taurus

Variables	Positive (%)	Negative (%)	Total (%)	P
Neospora
Cows	25 (41.6)	35 (58.4)	60 (50)	1.01
Pregnant	26 (43.3)	34 (56.7)	60 (50)
Non-pregnant	51 (42.5)	69 (57.5)	120 (100)
Trimester of gestation
First	7 (50.0)	7 (50.0)	14 (23.3)
Second	10 (35.7)	18 (64.3)	28 (46.7)	0.871
Third	8 (44.4)	10 (55.6)	18 (30.0)

Cow no.	ELISA1		Age of fetus (months)	PCR
	Cow	Fetus		Cows	Fetuses*
	Cow	Fetus		Blood	Brain	Myocardium
2	2.18	N	4	–	–	–
13	1.83	NO	1	+	–	–
25	2.25	N	7	–	–	–
26	2.17	N	3	–	–	–
28	0.55	N	6	–	–	–
53	3.05	N	6	–	–	–
54	0.6	N	7	–	–	–
58	1.13	3.3	8	–	–	–
62	0.59	NO	1	–	–	–
64	2.48	N	5	–	–	–
74	N	N	6	–	–	+
78	2.7	N	5	–	–	–
79	2.48	N	3	–	–	–
85	2.7	N	5	–	–	–
87	N	N	7	–	+	–
91	0.73	N	8	–	–	–
92	2.54	N	4	–	–	–
95	N	NO	2	+	–	–
97	0.74	NO	2	–	–	–
105	2.02	N	7	–	–	–
111	N	N	3	–	+	–
113	0.62	N	7	–	–	–
116	N	3.12	5	–	–	–
119	N	N	8	–	–	–
121	0.574	N	3	–	–	–
124	0.50	N	4	–	–	–
125	2.7	3.3	7	–	–	–
126	2.16	N	6	–	–	–
130	2.7	N	5	–	–	–
131	0.55	N	7	–	–	+
133	0.55	N	3	–	–	–

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Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

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Chi-square with Yates correction. % percentage.