The objective in this study was establish long-term conservation conditions for Passiflora setacea seeds by testing different seed moisture contents and storage temperatures. Seeds were treated with potassium nitrate (KNO3) and gibberellic acid (GA³) before germination in moistened paper towels. Seed viability was verified by the tetrazolium test after the seeds had been treated. It was observed that: (i) P. setacea seeds can be dried to approximately 4.7% of water content (fresh weight basis) without a significant reduction in germination, (ii) low temperatures and low water content induced dormancy in P. setacea seeds; (iii) dormancy could be overcome by seed treatment with gibberellic acid until the fifth month of storage; (iv) seeds maintained at subzero temperatures showed a higher viability compared to those stored at 4 °C. It was concluded that P. setacea seeds can be stored for up to eight months, at - 20 or -196 °C, since they maintain the minimum international standards for viability.
passionflower; viability; conservation; gibberelic acid
