Chemical treatment of roughage

Chemical treatment of roughage has been the aim of studies for a long time. However, until nowadays, many questions are arisen due to the efficiency of additives used in relation to the variation of answers, either in nutritive value of treated roughage or in performance of animals feed with diet containing such roughage. This revision will approach the main and most used products, which are anhydrous ammonia, urea, sodium hydroxide and calcium oxide. It has been noticed that ammonization (both for anhydrous ammonia and urea) has presented more efficient result in animal’s performance when compared with sodium hydroxide or calcium oxide. However, regarding its nutritive value, both sodium hydroxide and calcium oxide have presented a higher efficiency in the decreasing of cell wall and in the increase of treated roughage’ digestibility.


Introduction
The search for alternative foods for ruminants beyond pasture, silage and hay is a reality in Brazil's production system.Therefore, residues and sub-products assume a great importance in animal's production system, since that supply is the most difficult item on this system.In the case of silage and hay production, those could be produced with the own surplus of forage's production during the raining season, while residues and sub-products would depend of region's availability.In spite of have potential to supply some needs of ruminants, the residues and sub-products commonly have a poor nutritive and protein values and a high fiber value.
Chemical treatment then appears as an option to improve the nutritive value of forage.Many research works are mentioning the benefits of this practice (Pires et al., 2004;Bertipaglia et al., 2005;Ezequiel et al., 2005;Pires et al., 2006a,b;Ribeiro et al., 2009).
Chemical treatment of roughage is not a recently thing.Fingerlin et al. (1923) mentioned by Homb (1984) already have reported increases on organic matter digestibility of rye straw when treated with sodium hydroxide (NaOH).However, researches with animal's performance data and costs assessment must be done to adopt such practice.
In this revision we will discuss the aspects related to the chemical treatment of roughage, focusing ammonization (treatment with urea or anhydrous ammonia), treatments with sodium hydroxide or calcium oxide in different roughage that are potentially used for ruminants' feeding.

Conservation
Among the chemical products used for forages conservation, urea and anhydrous ammonia (NH 3 ) are the R. Bras. Zootec., v.39, p.192-203, 2010 (supl. especial) most used ones.One of the reasons to use these products for fodder maintenance is due their fungicide and bacteriostatic effect that they have over the forage stored at moisture over 20%, and in addition of providing nonprotein nitrogen that can be used by rumen microorganism for microbial protein synthesis.Pires et al. (1990) verified that NH 3 doses of 2 and 3% were efficient for the conservation of ground corn at high moisture while the untreated material or just treated with NH 3 1% visually presented fungus.
We had noticed that normally the addition of NH 3 doses over 1% (%DM) is enough for material's conservation.On the other hand, for the conservation of materials using urea, we need to use doses over 4% (%DM).
Chemical products as sodium hydroxide (NaOH) and calcium oxide (CaO) can also be used for forage's conservation.In a recent work made by Ferreira (2005), the author verified that the addition of 4% of NaOH or urea was enough to keep the brix degree of silage in sugar cane, presenting values of 16.1 and 10.7 while sugar cane in natura presented a brix degree value of 17 (Table 1).
The association of these two additives (2% NaOH + 2% urea) was also efficient for the conservation of brix degree, confirming the value of 15 units.Balieiro Neto et al. (2007), evaluating CaO doses (0, 0.5, 1 and 2%, based in NM) as additive for sugar cane silage, verified that the CaO dose of 2% was the one that promoted a higher recovery of dry matter (88.8%).According to these authors, the higher recovery of dry matter means the maintaining of silage's nutritive value when compare to original forage.With these results, we noticed that CaO presents potential to be used as additive for the quality maintenance of sugar cane silage.

Roughage treated with anhydrous ammonia (NH 3 ) or urea
Ammonization both with NH 3 and urea has presented satisfactory results both in the modification of chemical composition as in animals' performance.However, some factors are responsible for higher or lower ammonization efficiency and those factors can influence the process in isolation or combination.The main factors that will be discussed are: nitrogen doses that are applied, treatment periods, ambient temperature, moisture of material to be treated, quality of material, and nitrogen source.
Nitrogen dose was one of the first factors assessed in the study of roughage's ammonization.And in general it has been observed that NH 3 doses over 4% and urea doses of 7.5% normally are not used, because from these doses there is no longer response in quality's improvement.Besides, the highest nitrogen losses occur through the volatilization, when silos and stacks were opened.However, these values are used when we need to improve forage because such forage presents a poor digestibility.Lower doses of NH 3 and urea are used when we want to maintain forage with moisture over 18% and below 50%.In such case we normally use NH 3 at 1.0 to 1.5% and urea at 1.9 to 2.8% based in forage's dry matter.
Another factor is the treatment period, which is closely linked to ambient temperature.Literature has been reported that at higher temperatures, around 30°C, the ammonization period can be a week while when the temperature is lower as 5°C, it would take at least two months for ammonization be effective.
In a research work made by Reis et al. (1991), aiming to verify the effects from two ammonization periods (30 and 45 days) and four NH 3 doses (0, 2.0, 4.0 and 6.0%) over the chemical composition of Brachiaria decumbens and Melinis minutiflora hays and that were harvest at postflowering stage, they verified that CP and ADIN values increased due ammonization, specially for NH 3 doses of 4.0 and 6.0%.Regardless NH 3 doses, they did not notice any effect from ammonization period over nitrogen's retention in hays of both species, probably due the fact that the difference between ammonization periods is small.Authors still reported that the average ambient temperature of 20.5°C during the ammonization period allowed an adequate reaction between ammonia and treated material with 30 days of ammonization.
Material's moisture is another factor that has a remarkable effect because ammonia has high affinity with water.However, if the material has high moisture as silage, Averages followed by the same lowercase/uppercase in a same line/column do not differ by Tukey test of probability at 5%. the material's compression must be taken into account due a low NH 3 diffusion when applied on silos.Neiva et al. (1998a) reported these problems when they submitted corn silage containing 35 and 45% of DM to 1.2% of NH 3 (DM base).On the other hand, materials as hays and straws have presented less nitrogen retention when ammonized.We must highlight the moisture importance in material treated with urea, which in addition to urease enzyme also needs moisture for ureolysis that will result in NH 3 production.The minimum moisture generally recommended by literature is around 30%, and forage up to 50% of moisture can be used with satisfactory results.
According to Dolberg (1992) the major efficiency in urea treatment can be obtained when forage has moisture value around 30% and urea is applied at 4.0 to 8.0% dosage of roughage dry matter.
Another factor that affects ammonization response is the nutritive value.Forages presenting poor nutritive value when ammonized usually present better results when compared to forages presenting high nutritive values.This is because that both present high digestibility and low levels of cell wall constituents.So, it is evident the cell wall decrease and digestibility increase on roughage that have high NDF levels and low digestibility.
On grasses and their by-product the ammonization, it has a remarkable effect, especially regarding the digestibility increase whereas in legumes this effect is not observed.This is due the fact that most links found on grasses are ester while other plants present most ether-type links.Ammonia, due its ability of break ester-type links will present better results on grasses or their residues, like demonstrated in the work leaded by Pires et al. (2004) who ammonized sugar cane bagasse and noticed variations on digestibility around 32.1% for bagasse without treatment and 59.8% for bagasse treated with 4% of NH 3 .
The nitrogen source that will be used is another factor that reflects ammonization efficiency.NH 3 has shown to be more efficient than urea both for maintenance and improvement of nutritive value.However, its acquisition is harder when compared to urea.But when urea is used, moisture content, treatment period, ambient temperature and urease presence must be considered.So, the success of roughages treatment with urea as ammonia source depends on a fast hydrolysis followed by the ammonia contact with forages, which is dependent of moisture content in the material that will be treated and the presence of active urease.Urea is broken for ammonia production and sometimes the addition of some urease and water sources is necessary.Ideal moisture for the treatment with urea of low quality forages ranges from 30 to 50% (Dolberg, 1992;Sarmento et al., 2001).Reis et al. (1997) using NH 3 (0.5 and 1.0% DM base) and urea (0.9 and 1.8% DM base) for the treatment of Cynodon dactylon hay with high moisture and ammonized during 65 days, noticed an increase on in vitro digestibility of dry matter while for no-treated hay (moisture of 12-15% ) IVDMD was 46.5% and for treated hay (moisture of 20-25%) with NH 3 (0.5 and 1%) and urea (0.9 and 1.8%), the values founded were 59.0, 56.3, 51.4 and 51.1% respectively, showing a better NH 3 efficiency when compared to urea.
The attention for the treatment with NH 3 or urea is due the great potential that both have to improve the nutritive value of roughages since they act over the fibrous part breaking the chemical bands between lignin and hemicellulose.In addition to hemicellulose solubilization, there's a partial break in celluloses structure due the break of hydrogen bands.According to Garcia & Pires (1998), all these reactions occur due a fiber expansion and they facilitate the access of cellulase enzyme to substrate, improving the digestion process.For NH 3 use in roughages treatment, Garcia & Pires (1998) highlighted that there is an increase in the crude protein content in forages (two or three times), in digestibility (8 to 12 percentage units), in consume (average of 20%) and also in fungus control.
Ammonization (using NH 3 or urea) promotes an improvement of nutritive value in different roughages, increasing levels in CP values and decreasing NDF values (Table 2) and also promotes the increasing of in vitro dry matter digestibility (IVDMD) (Table 3).
In a work made by Carvalho et al. (2006), the authors evaluated urea dosages in the treatment of sugar cane bagasse and verified an increase on CP content, 3.7, 6.9, 9.9 and 12.9% and a decrease on NDF content, 78.1, 71.3, 64.4 and 57.6, respectively, for urea doses (%DM) of 0, 2.5, 5.0 and 7.5%.For IVDMD, the authors correlated treatments without addition of urea and with the addition of 7.5%, and they noticed an increase of 15.9 percentage units for treated bagasse.Pires et al. (2004), also testing the viability of using chemical products in roughages that present poor quality among other treatments, applied 4% of NH 3 on sugar cane bagasse and verified an increase on material's IVDMD, 32.1 and 59.8% for treatments without NH 3 and with an addition of 4%.
According to Reis et al. (2002), the annual crop residues from summer and winter, which are hays or grass silage harvested in advanced development stage from the harvest  of tropical forage grass seeds by the scanning method, are also other forage options for ruminants feeding, however they present a poor nutritive value.
On Table 2 is presented some results where were doses of NH 3 or urea were applied on Brachiaria hays and on rice straws.It is noticed an increase on CP contents and a decrease of NDF in all works showing that such chemical additives are effective to improve the nutritive level of these roughages.This efficiency can be even more ratified by IVDMD values showed at Table 3, which meaningfully increase for treated materials.Pires et al. (2006b), when they made the treatment of Brachiaria brizantha hay with NH 3 and urea doses, noticed that NH 3 was more efficient in increase material's IVDMD.Hay IVDMD values were 34.3, 52.9, 35.7 and 39.9%, respectively, for control with 3% of NH 3 , 3 and 5% of urea, treatments.The greater efficiency of NH 3 regarding urea has been frequently discussed in research works.For urea application, the presence of urease enzyme and moisture are essential for the urea breaking and release of ammonia.As NH 3 is already find under gas form and is readily available to be used, it acts in a most efficient form over fibrous compounds promoting higher IVDMD values in roughage.
It is noticed that some factors as ambient temperature, forages' moisture content, quantity of ammonia or urea added, treatment period and roughage chemical characteristics should never left out because they directly affect the treatment's efficiency.
As reported, rice and wheat straws, and other annual crops can be used for animal's feeding.The nutritive value of rice straw was studied by Cardoso et al. (2004), who noticed a IVDMD increase using 3% of NH 3 (%DM).The authors noticed values of, respectively, 43.0 and 59.0% of IVDMD for NH 3 doses of 0 and 3%.
NH 3 and urea on silages were also been successfully used.Recent works show the increase of CP contents and the decrease of NDF on silages treated with these chemical compounds.Fernandes et al. (2009), when applied urea during sorghum ensilage noticed values of 7.9, 15.9, 23.8 and 31.8% for CP and 64.4,61.1, 60.3 and 59.3% for NDF, respectively, for urea doses of 0, 2.5, 5.0 and 7.5% (Table 4).
The digestibility both in vitro and in vivo increases with the addition of NH 3 or urea.The benefits observed on digestibility of these materials are directly related with the fiber's reduction and expansion, once that such products cause a softening in cell wall compounds in treated forages allowing a more pronounced action of microbial enzymes existent in rumen (Garcia & Pires, 1998).
The NDF digestibility in goats fed Brachiaria decumbens hay treated with NH 3 or urea increased with Pires et al. the addition of those products (Rosa et al., 1998), and it was observed that the benefits of NH 3 and urea were similar and also that treated hays presented higher digestibility than not treated hays (Table 5).The digestibility values observed by authors were 60.1, 70.7, 69.6, 67.4 and 68.3%, respectively, for NH 3 doses of 0. 2 and 3% and urea doses of 3.6 and 6.4%.
According to Neiva et al. (1998b), the benefits of chemical treatment of forages are relevant.The authors verified a increase of 10 percentage units to NDF digestibility in bovine fed corn silage with application of 1.2% of NH 3 (%DM).In the same work, the authors tested the addition of 2.4% of NH 3 to corn fodder and they verified an increase of 11.5%.In a work with Pennisetum purpureum silage, Silva et al. (2007) added 5% of urea (%DM) during the grass ensilage and noticed an increase in NDF digestibility when they supplied such diet for Santa Inês sheep.It was reported 39.2 and 55.2% values, respectively, for urea doses of 0 and 5%.
As Rosa et al. (2001) the high digestibility values in forages treated with NH 3 or urea comes from the marked changes in chemical composition, especially due the relation of fraction of nitrogen compounds and cell wall.
On Table 6 are shown the consume values for dry matter in % live weight (LW) and average daily gain (kg/day); these data was collected of works complied from literature of animals fed forage treated with NH 3 or urea.
All benefits from chemical treatments previously reported cause a direct effect over animals' intake and weight gain, once that the main purpose of the treatment is to make the roughage more enjoyable through modifications on cell wall, allowing a higher efficiency of microorganisms over substrate.According to Reis et al. (2002), we must take into account that treatment can increase the nutrient content of forage, which will contribute to increase its efficiency of use.
On works made by Pires et al. (2003Pires et al. ( , 2004)), the authors observed a higher consume from animals fed roughages treated with NH 3 .On this work, among other treatments, it was used sorghum silage treated with 2.5% of NH 3 (%DM) and sugar cane bagasse treated with 4% of NH 3 (%DM), respectively (Table 6).
Bovine's intake and weight gain were evaluated by Cardoso et al. (2004), who supplied rice straw treated with NH 3 to beef cattle.The authors verified a higher intake and weight gain on animals that received straw treated with 3% of NH 3 (Table 6).

Roughages treated with sodium hydroxide (NaOH) and calcium oxide (CaO)
The roughage is considered as the lowest cost compound in ruminants' diet and its nutritive quality is extremely important and it is inversely related to the need of concentrates on diet.As reported, the forages' seasonality can be alleviated with the use of forage in its maintained form.
Among the roughages for ruminants, sugar cane has been highlighted in Brazil's agribusiness scenario and during the recent years its cultivation has been even more expanding.The high productivity of green mass (80 to 120 t/ha), the low cost by dry matter unit, the maintenance of nutritive value for up to six months after maturation and the harvest period coincides with scarcity period of forages in pastures combined with the facility of obtaining seedlings and planting and the possibility of reach reasonable weight gain rates has attracted ranchers to use sugar cane as a source of roughage food for bovine feeding.
The great adoption of sugar cane as a supplementary roughage for dry periods is based in its ease of culture and tradition, in its use under fresh form through daily cuts, and it is traditionally well-known by produces.This daily manpower is always questioned by producers, mainly large, which feed large herds.
However, despite the widespread use of sugar cane, constant reports on literature indicate that it has a low digestibility that causes direct implications on nutrients intake by animals, resulting in a low productivity of animals during dry season (Vilela et al., 2007), which is a period that animals most need this roughage.Due such reasons, the search for a solution to improve this roughage's quality and, consequently, the animals' performance has been intensified during the last years.
Although NaOH has been studied as additive for straws and hays with poor quality during the last decades, nowadays we can notice that sugar cane is the forage that has received more attention of researches when the objective is NaOH treatment (Pires et al., 2006a;Freitas et al., 2006;Ribeiro et al., 2009).
Another additive that has been largely used for sugar cane treatment is CaO, which has been applied in sugar cane during its several processing forms.The CaO indicated for use in roughages' treatment is microprocessed or micropulverized lime, which is a product with mineral origin that passes through an industrial process and acquires an appearance of fine material (Martines, 2006).
We can notice the use of additives during sugar cane silage.The loss from sugars reduction during sugar cane silage can reach 30% with a consequent production of ethanol, due this fact that the additive is used during ensilage.
During the last years, the use of NaOH and CaO additives has been directed for sugar cane treatment.So, the chemical treatment using alkaline additives to improve the quality of forages is an interesting alternative to potentiate the use of these foods by ruminants.
The addition of NaOH or CaO in roughages, different from NH 3 and urea chemical compounds, does not cause alterations in nitrogen compounds.This is because its own composition does not contain any nitrogen element.Tables 7, 8 and 9 can be verified CP contents of roughages treated, respectively, with NaOH and CaO.
For NDF contents, the addition of NaOH causes meaningful reductions in these fractions; this is also observed in works employing the addition of CaO.Evaluating the addition of NaOH or CaO doses of 0, 0.75, 1.5 and 2.25 to sugar cane, Ribeiro et al. (2007) noticed reductions on NDF contents, verifying NDF values of 59.4, 56.3, 50.2 and 46.4%, and 58.9, 56.6, 51.8 and 47.9%, respectively, for NaOH and CaO applications.Similar results were also obtained by Siqueira et al (2007), which are related to NaOH dose of 1% during sugar cane silage.They verified decreasing of 16 percentage units in NDF contents in comparison to not treated silage.In a work leaded by Lopes et al. (2007), the authors assessed the use of NaOH in Pennisetum purpureum and the results were below the values observed for sugar cane, as can be verified in the works of Freitas et al. (2006) and Pedroso et al. (2007) (Table 7).
Sugar cane, under different processing forms was studied by Ezequiel et al. (2005).The authors tested sugar cane in natura cut and supplied on time, sugar cane hydrolyzed with NaOH during 24 hours, hay hydrolyzed sugar cane and silage hydrolyzed sugar cane, and in all treatments was used 1.5% of NaOH applied at NM base.As already verified in other works, CP contents did not present great variations, NDF contents decreased, and IVDMD  increased with NaOH addition.According to the presented results, it appears that hydrolyzed sugar cane by 24 h and hay hydrolyzed sugar cane presents best results, once that such treatment allowed a large DM intake (Table 9).
For IVDMD values of materials treated with NaOH, it is noticed that in every situation there is an increase.According to the results presented on Table 10, everything indicates that the application of 1 to 2% of NaOH is enough to promote good results in IVDMD.
In sugar cane works, we can observe that the addition of CaO is made both for disintegrated sugar cane resting for 24 hours as during ensilage.The reduction of NDF contents is noticed in both ways of using sugar cane, demonstrating that CaO is a hydrolyzing additive with potential action in different environments.
One factor that may affect sugar cane hydrolysis is the treatment period.According to Martinez (2006), sugar cane must be submitted to a period of at least 3 to 24 hours in order that hydrolysis occurs in an efficient way.
Besides, another important factor is material's moisture, which is necessary for calcium hydroxide formation.As Moraes (2006), it is necessary 1.0 mol of H 2 O for each CaO mol to calcium hydroxide formation (Ca(OH) 2 ); in other words, for each 56.0 g of lime is necessary 18.0 g of water.Cavali (2006) evaluated CaO doses of 0, 0.5, 1.0, 1.5 and 2.0% of CaO during sugar cane ensilage and verified a reduction in NDF contents and an increase of IVDMD.According the results presented by the author, IVDMD increased 35.5, 54.8, 61.6 and 68.4%, respectivelly for treatments, and 0.5, 1.0, 1.5 and 2,0% regarding control treatment without CaO addition.
In a work made by Balieiro Neto et al. (2007), the authors also verified expressive increases on IVDMD with the addition of CaO during sugar cane ensilage (Table 11).The IVDMD increase, which was noticed by these authors demonstrated CaO efficiency as additive reducing the constituents of roughages' cell wall and improving digestibility.Ribeiro et al. (2007) noticed a reduction in lignin contents when they hydrolyzed sugar cane with CaO doses of 0, 0.75, 1.5 and 2.25% (%NM) stored during 24 hours.In the same way, Balieiro Neto et al. ( 2007) also noticed a lignin reduction with CaO application.The authors noticed such difference from CaO doses of 2% (%NM).Lignin is a compound that is negatively correlated with digestibility, so the reduction of such compound on materials treated with CaO may reinforce the IVDMD increase in the works that were previously mentioned.Just some works have evaluated the use viability of sugar cane treated with CaO in ruminants feeding.So, until now it is hard to affirm that sugar cane treated with CaO does not affect the animal perfomance (intake, weight gain and milk production).Carvalho (2008) gave diets with sugar cane treated with CaO doses of 0, 0.75, 1.5 and 2.5 (%NM) to lactating cows and did not verify a difference for milk production (Figure 1), recording productive averages around 8.0, 7.7, 7.8 and 7.3 kg of milk/animal/day, respectively.
More studies are necessary to reach a definitive conclusion regarding the use of CaO in sugar cane hydrolysis.
On Table 12 are shown averages of dry matter intake and average daily gain of bovine, sheep and goats fed sugar cane treated with CaO.
The results still show a data inconsistence once that present results with worst performance and results with better performance when sugar cane is treated with CaO.
In a work leaded by Moraes (2006), it was verified less intake and less weight gain in heifers fed sugar cane treated with CaO 1% (%NM).Among the reasons for which the author tryed to explain such result, the low palatibility of treated material was mentioned with more emphasis.In other work using animals, Pancoti et al. (2007) tested 0.6% of CaO (%NM) in sugar cane treatment and noticed less dry   matter intake when compared to control treatment.But the data obtained by Carvalho (2008) presented a increase of intake both for ovine and goats (Table 12).The reasons for a different behavior between chemical analyses and animals performance when those are fed sugar cane treated with CaO may be due the alterations that occur during the pre-drying method on stove at 60°C during 72 hours.This pre-drying temperature can improve CaO efficiency in cell wall, reducing NDF and improving digestibility, once that the food supplied for ruminants is not submitted to this process.Another factor may be connected to calcium and phosphorus relation when forages are treated with CaO.This relation is increased to an amont that can affect ruminal microorganisms.Therefore, it is necessary to evaluate the calcium and phosphorus levels and to establish a relation of these compounds with ruminant's diet.

Final Considerations
The use of alkaline products maintains high moisture roughages, and also improves its nutritive value.
Factors as source and dose are responsible for the quality improvement degree of treated roughages.
Urea and anhydrous ammonia need more time to cause the cell wall breaking reaction in treated roughages, but such reactions supply non-proteic nitrogen, which will be used by ruminal bacteria.
Sodium hydroxide and calcium oxide promote an improvement in nutritive value of treated roughages in smaller periods than anhydrous ammonia or urea.
Calcium oxide is less efficient than sodium hydroxide on the improvement of nutritive value of treated roughages, however its handling is easier and less dangerous.
The calcium:phosphorus relation on roughages treated with calcium sodium must be better evaluated in works that also approach the animal performance.

Figure 1 -
Figure 1 -Milk production (kg/day) of cows fed hydrolyzed sugar cane with different calcium oxide (CaO) doses.

Table 1 -
Average values of brix degree of silage in two sugar cane varieties that were submitted to different additives

Table 2 -
Crude protein content (CP) and neutral detergent fiber (NDF) of roughages treated with different doses of anhydrous ammonia (NH 3 ) and urea 1 Dose applied in percentage of dry matter.

Table 3 -
In vitro dry matter digestibility (IVDMD) of roughages treated with different doses of anhydrous ammonia (NH 3 ) and urea

Table 4 -
Crude protein contents (CP) and neutral detergent fiber of silages treated with different doses of urea and anhydrous ammonia (NH 3 ) 1 Dose applied in percentage of natural matter (NM) (sugar cane with 28% of dry matter [DM]). 2 Dose applied in percentage of NM (authors did not mention the sugar cane DM content). 3se applied in percentage of NM (sorghum with 30.1% of DM).4Dose applied in percentage of DM. 5 Dose applied in percentage of NM (Pennisetum purpureum with 15.3% of DM). 6 Dose applied in percentage of NM (authors did not mention the sunflower DM value).

Table 5 -
Neutral detergent fiber digestibility (NDFD) of roughages treated with different doses of anhydrous ammonia (NH 3 ) and urea

Table 6 -
Dry matter intake (DMI), in % live weight (LW) and average daily gain (ADG) in kg/day of bovine fed roughage treated with different doses of anhydrous ammonia (NH 3 ) and urea

Table 7 -
Crude protein (CP) contents and neutral detergent fiber (NDF) of roughages treated with sodium hydroxide (NaOH) 1 Dose applied in percentage of natural matter. 2Dose applied in percentage of dry matter.

Table 8 -
Crude protein (CP) contents and neutral detergent fiber (NDF) of roughages treated with calcium oxide (CaO) 1 Dose applied in percentage of natural matter.

Table 9 -
Crude protein (CP) contents and neutral detergent fiber (NDF), in vitro dry matter digestibility (IVDMD) and dry matter intake (DMI) in % weight live (WL) of steers fed sugar cane treated with sodium hydroxide processed in different forms

Table 10 -
In vitro dry matter digestibility of (IVDMD) of roughages treated with sodium hydroxide (NaOH)

Table 11 -
In vitro dry matter digestibility of (IVDMD) of roughages treated with calcium sodium (CaO) 1 Dose applied in percentage of natural matter.

Table 12 -
Dry matter intake (DMI) in % weight live (WL), and average daily gain (ADG) of roughages treated with different calcium oxide (CaO) doses