Deposition of conjugated linoleic acid (CLA) in Nile tilapia


Objetivou-se avaliar a deposição de ácido linoléico conjugado (CLA) em filés de tilápia-do-nilo (Oreochromis niloticus). Foram utilizados peixes com 121,08 ± 8,48 g, distribuídos em quatro caixas de cimento amianto (1,0 m³ cada uma) e arraçoados até saciedade aparente, duas vezes ao dia, durante 49 dias. Como alimento, utilizou-se ração comercial extrusada com 28% de PB e 3.000 kcal de energia digestível/kg, acrescida de 2% do produto comercial Luta-CLAâ (BASF Brasil). No início do experimento, cinco peixes foram coletados aleatoriamente como amostra controle (sem CLA). A cada sete dias, dois peixes de cada caixa foram coletados, pesados e filetados para determinação da taxa de deposição de CLA nos filés. As amostras de filés foram estocadas a -24ºC para análise do teor de lipídios totais e do perfil de ácidos graxos. A taxa de deposição de CLA foi determinada pela função de Gompertz (y = a . exp{-b . exp[-kt]}). O meio-tempo (T) para deposição de 50% do CLA foi estimado pela equação T = ln2/k, em que: T representa o meio-tempo; ln, o logaritmo Neperiano e k, a unidade de tempo que fornece a "velocidade" de deposição do CLA no tecido muscular da tilápia-do-nilo. A deposição máxima de CLA nos lipídios totais do filé de tilápias-do-nilo estimada pela função de Gompertz foi de 1,61 g/100 g de lipídios do filé. O meio-tempo de deposição de CLA nos lipídios do filé ocorre com 6,86 dias.

ácidos graxos; ácido linoléico conjugado; Oreochromis niloticus

This study was carried out to determine the deposition of conjugated linoleic acid (CLA) in Nile tilapia Oreochromis niloticus fillet. Fish averaging 121.08 ± 8.48 g were distributed in four cement amianthus tank (1.0 m³ each) and hand fed to apparent satiation, two times a day for 49 days. As feed, an extruded commercial diet with 28% CP and 3000 kcal of digestible energy was utilized. A commercial CLA product (Luta-CLAâ, BASF) was added at 2% of the diet. At the beginning of the experiment, five fish were randomly collected as a control sample (without CLA). Weekly, two fish of each tank were collected, weighed and filleted for measurement of CLA deposition ratio in the fillets. The fillet samples were stored at -24ºC for total lipid and analyses of fatty acids profiles. The deposition rate of CLA was determined by Gompertz function (y = a . exp{-b . exp[-kt]}). Half time (T) to have 50% deposition of CLA was calculated by the equation T = ln2/k, where T is half time; ln is Napierian logarithm and constant k the time unit, which provides the "velocity" in the deposition of CLA in the Nile tilapia fillets. The maximum CLA deposition in the fish fillet lipids, estimated by the Gompertz function, was 1.61 g/100 g fillets lipids. Half time of CLA deposition in the fillet lipids occur within 6.86 days.

conjugated linoleic acid; fatty acids; Oreochromis niloticus


Oswaldo Paulo Forattini, one of the founders of the Revista de Saúde Pública and its Editor for a continuous 40-year period, passed away on September 15, 2007, in the city of São Paulo, at the age of 83 years. Tenaciously and obstinately, in the same way in which he made so many contributions within the fields of epidemiology and medical entomology, he fought to construct something that, at the outset, he knew was just a utopian dream: a Brazilian scientific journal for public health with international prestige. But he achieved it. Always supported by the Faculdade de Saúde Pública, he had assistance in the early days from his colleagues in the Faculdade, who formed part of what was then called the "Publications Committee" of the Revista de Saúde Pública. Much later on, in 1989, in an action that was a determining factor in the growth of the journal, he proposed to create a Board of Editors, in which renowned researchers from the whole country would participate.

Professor Forattini left his mark as a demanding and rigorous editor with regard to evaluations: for him, quality was a non-negotiable target. No published study failed to be subjected to screening by specialists, without exceptions. He obstinately pursued the target of gaining indexation for the Revista de Saúde Pública in the main worldwide bibliographic control system and, in 1982, after years of argument and great insistence, he achieved indexation for the journal in the Social Science Citation Index of the then Institute of Scientific Information (ISI), which today is known as Thomson Scientific. Even today, this is the goal of all scientific journals that crave international recognition. Thanks to his tireless work, all of the more than 2,864 articles published by the Revista de Saúde Pública have now been indexed in Medline.

Although his personal preference was to keep far away from computers and modern electronic technology, he was always receptive towards implementing changes that would contribute towards modernizing the Revista. Thus, in 1997, in another action that catapulted the growth of the Revista de Saúde Pública upwards, he supported the founding of the SciELO network and the immediate entry of the journal into this important Latin American electronic collection with free access to complete texts that is coordinated by Bireme and Fapesp. Today, ten years later, the Revista de Saúde Pública is one of the SciELO-indexed periodicals that is most accessed and cited.

In addition to his vast bibliographic contribution coming from the results of his studies as an epidemiologist and entomologist, Professor Forattini was a proficient editorialist, filling the pages of his journal with texts on polemical topics that are still very current, like the following:

Terá sido a erradicação mundial da malária empreendimento insensato? [Was malaria eradication a foolish enterprise?]

Rev Saude Publica.1980;14(3):263-4.

A poliomielite e seus enigmas. [Poliomyelitis enigmas]

Rev Saude Publica.1981;15(2):111-2.

Por que saúde pública? [Why Public Health?]

Rev Saude Publica.1983;17(2):69-70.

Varíola e erradicação? [Smallpox and eradication?]

Rev Saude Publica.1985;19(5):385-8.

Febre amarela e vacinação. [Yellow fever and vaccination]

Rev Saude Publica.1986;20(6):411-4.

Varíola, erradicação e doenças infecciosas. [Smallpox eradication]

Rev Saude Publica.1988;22(5):371-4.

Biossistemática e saúde pública. [Biosystematic and Public Health]

Rev Saude Publica.1989;23(3):181-2.

Epidemiologias, ou epidemiologia? [Epidemiologies and Epidemiology]

Rev Saude Publica.1990;24(1):1-2.

"Limitações" da teoria ecológica da saúde e doença. [Ecology theory limitations on health and disease]

Rev Saude Publica.1990;24(2):94.

Aids e sua origem. [AIDS and its origin]

Rev Saude Publica.1993;27(3):153-6.

A Universidade e a pesquisa. [The University and research]

Rev Saude Publica.1994;28(3):175-6.

A educação é realmente imprescindível para o desenvolvimento? [Is education really indispensable for development?]

Rev Saude Publica.1994;28(4):245-6.

Universidade e tecnologia. [University and technology]

Rev Saude Publica.1994;28(5):327.

Pesquisa em saúde pública. [Research in Public Health]

Rev Saude Publica.1995;29(2):81-8.

Pesquisa no Brasil? [Research in Brazil, are there?]

Rev Saude Publica.1995; 29(3):157-8.

A Universidade e a eletrônica. [The University and the Electronics]

Rev Saude Publica.1995;29(6):425-7.

Quem, onde, quando, o quê e o porquê. [Who, where, whne, what and why]

Rev Saude Publica.1996;30(6):499-502.

A Internacionalidade da ciência. [The international nature of science]

Rev Saude Publica.1997;31(5):115.

No Corredor da morte. [On the death row]

Rev Saude Publica.1999;33(1):3-5.

A comunidade européia, o euro e a ciência. [The European community, the euro and the science]

Rev Saude Publica.1999;33(3):217-8.

Febre amarela. [Yellow fever]

Rev Saude Publica.1999;33(6):534.

A ciência e as necessidades básicas. [Science and the basic human needs]

Rev Saude Publica.2000;34(2):109-10.

A saúde pública no século XX. [Public Health in the 20th century]

Rev Saude Publica.2000;34(3):211-3.

Reemergência de infecções. [Infections resurgence]

Rev Saude Publica.2000;34(2):111-2.

A privatização da universidade e o genoma. [The university privatization and the genome]

Rev Saude Publica.2001;35(2):111-2.

Doenças não transmissíveis e infecções. [Non-transmitable diseases and infections]

Rev Saude Publica.2002;36(1):1-3.

A volta da vacina. [Vaccine again]

Rev Saude Publica.2002;36(4):383-4.

On behalf of the dozens of editors who have worked with him on the Revista de Saúde Pública, the hundreds of authors who were able to have their work known worldwide and the thousands of readers who were able to make use of these studies, we salute Oswaldo Paulo Forattini, a man of his time who served Science and his country well.

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  • Oswaldo Paulo Forattini - editor
    The Editors

Publication Dates

  • Publication in this collection
    30 Nov 2007
  • Date of issue
    Oct 2007


  • Accepted
    26 Mar 2007
  • Received
    12 Dec 2005
Sociedade Brasileira de Zootecnia Universidade Federal de Viçosa / Departamento de Zootecnia, 36570-900 Viçosa MG Brazil, Tel.: +55 31 3612-4602, +55 31 3612-4612 - Viçosa - MG - Brazil